Nog (NM_008711) Mouse Tagged ORF Clone

CAT#: MR225276

  • TrueORF®

Nog (Myc-DDK-tagged) - Mouse noggin (Nog)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_008711" in other vectors (6)


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Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

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Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Nog
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR225276 representing NM_008711.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGCGCTGCCCCAGCCTGGGGGTCACCCTCTACGCCCTGGTGGTGGTCCTGGGGCTGCGGGCAGCA
CCAGCCGGCGGCCAGCACTATCTACACATCCGCCCAGCACCCAGCGACAACCTGCCCCTGGTGGACCTC
ATCGAACATCCAGACCCTATCTTTGACCCTAAGGAGAAGGATCTGAACGAGACGCTGCTGCGCTCGCTG
CTCGGGGGCCACTACGACCCGGGCTTTATGGCCACTTCGCCCCCAGAGGACCGACCCGGAGGGGGCGGG
GGACCGGCTGGAGGTGCCGAGGACCTGGCGGAGCTGGACCAGCTGCTGCGGCAGCGGCCGTCGGGGGCC
ATGCCGAGCGAGATCAAAGGGCTGGAGTTCTCCGAGGGCTTGGCCCAAGGCAAGAAACAGCGCCTGAGC
AAGAAGCTGAGGAGGAAGTTACAGATGTGGCTGTGGTCACAGACCTTCTGCCCGGTGCTGTACGCGTGG
AATGACCTAGGCAGCCGCTTTTGGCCACGCTACGTGAAGGTGGGCAGCTGCTTCAGCAAGCGCTCCTGC
TCTGTGCCCGAGGGCATGGTGTGTAAGCCATCCAAGTCTGTGCACCTCACGGTGCTGCGGTGGCGCTGT
CAGCGGCGCGGGGGTCAGCGCTGCGGCTGGATTCCCATCCAGTACCCCATCATTTCCGAGTGTAAGTGT
TCCTGC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAG
GTTTAA
>Peptide sequence encoded by MR225276
Blue=ORF Red=Cloning site Green=Tag(s)

MERCPSLGVTLYALVVVLGLRAAPAGGQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSL
LGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLS
KKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRC
QRRGGQRCGWIPIQYPIISECKCSC

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008711
ORF Size 696 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1922 bp
RefSeq ORF 699 bp
Locus ID 18121
UniProt ID P97466
Cytogenetics 11 54.34 cM
MW 25.8 kDa
Gene Summary Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (PubMed:9585504, PubMed:9603738). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.