Lmo2 (NM_001142335) Mouse Tagged ORF Clone

CAT#: MR225099

Lmo2 (Myc-DDK-tagged) - Mouse LIM domain only 2 (Lmo2), transcript variant 2

Product Images

Circular map for MR225099

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lmo2
• Synonyms: Rbtn-2; Rbtn2; Rhom-2; Ttg2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225099 representing NM_001142335
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGAGCGCGGTGACTGTCCTTGAGCGTGGAGGGGCGAGCTCGCCAGCGGAGCGACCGAGCAAGA
  GGAGGCGCAGGAGCGGCGGCGCCCGAGCACCCGAGGGGGTCCGAGTCCAGGCAGCTAGCCAGCCACGCGC
  CACAAAGGGAGCGCCCCCGCCGCCCGGCACGCCGCCCCCCTCCCCAATGTCCTCGGCCATCGAAAGGAAG
  AGCCTGGACCCGTCTGAGGAACCCGTGGATGAGGTGCTGCAGATACCCCCATCCCTGCTGACATGTGGTG
  GCTGCCAGCAGAACATAGGGGACCGCTACTTCCTGAAAGCCATCGACCAGTACTGGCATGAGGATTGCCT
  CAGCTGTGACCTCTGTGGGTGTCGGCTGGGAGAGGTGGGGAGGCGCCTCTACTACAAGCTGGGACGGAAA
  TTGTGCAGGAGAGACTATCTCAGGCTTTTTGGTCAGGATGGTCTCTGTGCATCCTGTGACAAGCGGATCC
  GTGCCTATGAGATGACGATGCGGGTGAAAGACAAAGTGTATCACCTGGAGTGTTTCAAATGCGCCGCCTG
  TCAGAAGCATTTCTGTGTAGGTGACAGATACCTTCTCATCAACTCCGACATAGTGTGTGAACAAGACATC
  TACGAGTGGACCAAGATCAATGGGATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225099 representing NM_001142335
  Red=Cloning site Green=Tags(s)
  
  MEGSAVTVLERGGASSPAERPSKRRRRSGGARAPEGVRVQAASQPRATKGAPPPPGTPPPSPMSSAIERK
  SLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRK
  LCRRDYLRLFGQDGLCASCDKRIRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDI
  YEWTKINGII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142335
• ORF Size: 660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142335.1, NP_001135807.1
• RefSeq Size: 1519 bp
• RefSeq ORF: 663 bp
• Locus ID: 16909
• Cytogenetics: 2 54.46 cM
• MW: 25 kDa
• Gene Summary: Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.[UniProtKB/Swiss-Prot Function]