Zkscan14 (NM_023322) Mouse Tagged ORF Clone

CAT#: MR224916

Zkscan14 (Myc-DDK-tagged) - Mouse zinc finger with KRAB and SCAN domains 14 (Zkscan14)

Product Images

Circular map for MR224916

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zkscan14
• Synonyms: 2310046C23Rik; 2810437E14Rik; Zfp99; Znf394
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224916 representing NM_023322
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGGCTCTGGGGTCGTCCCGCCACCTCTCGGTGCTGGGCTTTGTACGGTGAAGGTGGAGGAGG
  ACTCTCCGGGGAATCAGGAGTCCTCCGGTTCCGGAGATTGGCAGAACCCCGAAACTTCTCGAAAACAATT
  CAGGCAGTTGCGCTACCAGGAAGTGGCCGGCCCTGAGGAGGCGCTGAGCCGACTTTGGGAGCTCTGTCGG
  AGGTGGCTGAGGCCCGAGCTGCTCTCCAAGGAGCAGATCATGGAGCTGTTGGTGCTGGAGCAATTCCTCA
  CCATCCTGCCCCAGGAGCTCCAGGCCTACGTGCGGGACCACAGCCCTGAAAGTGGGGAGGAGGCTGCGGC
  CTTGGCGCGGACTCTGCAAAGAGCACTGGATCGGGCCTCTCCGCAGGGTTTTATGACCTTCAAGGATGTG
  GCTGAATCTCTGACCTGGGAAGAGTGGGAACAGCTGGCTGCAGCTCGGAAAGGTTTCTGTGAAGAGAGCA
  CAAAGGACGCTGGGAGCACAGTTGTGCCTGGCTTGGAAACACGAACTGTGAACACAGATGTGATTCTAAA
  GCAGGAAATTTTAAAGGAGGCAGAGCCACAGGCATGGCTACAAGAAGTATCCCAGGGAATGGTCCCAGCA
  CTTACCAAATGTGGTGACCCCTCTGAGGACTGGGAAGAAAAGCTGCCAAAAGCTGCTGTACTGCTGCAGC
  TCCAGGGTTCTGAAGAGCAAGGACGCACTGCCATCCCGCTTCTCATCGGTGTGTCCAGAGAGGAAAGGGA
  CTCTAAAAACAATGAGTCTGAGAACAGTGGCAGTTCTGTCCTTGGTCAGCACATCCAGACAGCAGAAGGA
  CTGGGCACCAACAGTCAGTGTGGGGATGACCACAAACAAGGTTTCCATGTGAAATGCCATTCAGTGAAGC
  CCCACAGCTCTGTGGACAGTGCTGTAGGGCTTCTTGAGACCCAGAGGCAGTTCCAGGAAGACAAACCTTA
  CAAATGTGATAGCTGTGAGAAGGGCTTCAGACAGCGCTCGGACCTCTTCAAACACCAGAGAATCCACACA
  GGTGAGAAGCCCTATCAGTGCCAAGAGTGTGGGAAACGCTTTAGTCAGAGCGCTGCCCTCGTTAAGCACC
  AGCGGACACACACAGGTGAGAAGCCATATGCATGCCCAGAATGCGGGGAGTGCTTCAGGCAGAGCTCACA
  TCTGAGTCGGCATCAGAGAACCCATGCCAGTGAGAAGTACTATAAATGTGAGGAGTGTGGGGAGATCGTT
  CATGTTTCCAGCCTGTTCAGACATCAGAGACTACACAGAGGGGAGAGACCATACAAGTGTGGAGACTGTG
  AGAAGAGCTTCAGACAGCGCTCAGACCTCTTCAAGCACCAGAGAACCCACACAGGAGAGAAGCCCTATGC
  GTGTGTCGTCTGCGGGAGAAGATTCAGTCAGAGCGCCACCCTCATTAAACACCAGAGAACTCACACAGGA
  GAAAAACCGTATAAATGTTTTCAATGTGGTGAAAGATTTAGACAGAGTACACACCTTGTCCGACACCAAA
  GAATCCATCAAAATTCAGTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224916 representing NM_023322
  Red=Cloning site Green=Tags(s)
  
  MAAGSGVVPPPLGAGLCTVKVEEDSPGNQESSGSGDWQNPETSRKQFRQLRYQEVAGPEEALSRLWELCR
  RWLRPELLSKEQIMELLVLEQFLTILPQELQAYVRDHSPESGEEAAALARTLQRALDRASPQGFMTFKDV
  AESLTWEEWEQLAAARKGFCEESTKDAGSTVVPGLETRTVNTDVILKQEILKEAEPQAWLQEVSQGMVPA
  LTKCGDPSEDWEEKLPKAAVLLQLQGSEEQGRTAIPLLIGVSREERDSKNNESENSGSSVLGQHIQTAEG
  LGTNSQCGDDHKQGFHVKCHSVKPHSSVDSAVGLLETQRQFQEDKPYKCDSCEKGFRQRSDLFKHQRIHT
  GEKPYQCQECGKRFSQSAALVKHQRTHTGEKPYACPECGECFRQSSHLSRHQRTHASEKYYKCEECGEIV
  HVSSLFRHQRLHRGERPYKCGDCEKSFRQRSDLFKHQRTHTGEKPYACVVCGRRFSQSATLIKHQRTHTG
  EKPYKCFQCGERFRQSTHLVRHQRIHQNSVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023322
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023322.2, NP_075811.2
• RefSeq Size: 1992 bp
• RefSeq ORF: 1566 bp
• Locus ID: 67235
• UniProt ID: Q9Z1D9
• Cytogenetics: 5 G2
• MW: 59.5 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]