Zbtb48 (NM_133879) Mouse Tagged ORF Clone

CAT#: MR224885

Zbtb48 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48)

Product Images

Circular map for MR224885

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zbtb48
• Synonyms: 0610011D15Rik; AI327031; Hkr3; TZAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224885 representing NM_133879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGTTCCTTCGTGCAGCACAGCGTGAGGGTTCTGCAGGAACTCAACAAGCAGCGCGAGAAGGGCC
  AGTATTGCGACGCCACACTGGACGTGGGGGGCCTGGTGTTCAAGGCGCACTGGAGTGTCCTGGCGTGTTG
  CAGCCATTTCTTCCAGAGAATCTACGGGGATGGCACAGGGGGCAGCGTTGTCCTCCCAGCGGGCTTTGCT
  GAGATTTTTGGCCTCCTGCTGGACTTTTTCTACACTGGCCACCTCGCCCTCACCTCAGGGAACAGGGATC
  AGGTACTCTTGGCAGCCAAAGAGTTGAGGGTGCCTGAGGCTGTGGAGCTATGTCAGAGCTTCCAGCCCCA
  AACCTCAGTGGGACAGGCACAGAGTGGACTGGGTCAACCTGCCTCCCAGGACGTGAAAAGCCACCTGAAG
  GAGCCGACAGACTTGGATGAGGAGGAAGTTTTCAGGACTCTGAGCCTGGCCTCCGTGGATCAGGAGCCCA
  GAGACACTGAGCAGCCCCAGCTGGGTACACCTGCTCAGAGCACCACGGCCTTCCTCTGTGGGAAACTCAC
  GCAGGCTTTGAAGCCTTCTCCCTCAGAGGACAAAGAGTCTGAGGACTGCAAAGAGCCCCCAAGGCCCTTC
  GAGGCTGGAGGTGCCCCGCTGCAGGGCGAGAGCAATGAGTGGGAAGTGGTGGTCCAAGTGGAGGACGACA
  GGGACGGTGATTACGTATCTGAGCCTGAGACTGTGCTAACCAGAAGGAAGTCAAAAGTGATTAGAAAACC
  CTGTGCAGCCGAGCCAGCCCTGGGTGCGGGTTCTCTCACAGCCGAGCCCACTGACAGCAGAAAAGGTGCA
  GCCGTTCCGGTCGAATGTCCCACATGTCACAAAAAGTTCCTCAGCAAATATTACCTGAAAGTCCACAACA
  GGAAACACACTGGGGAGAAACCCTTCGAGTGTCCCAAATGTGGGAAGTGTTACTTCCGCAAGGAGAATCT
  CTTGGAACATGAAGCCAGGAATTGCATGAACCGCTCAGAACAGGTCTTCACATGCTCCGTATGCCAGGAG
  ACCTTCCGACGGAGGATGGAGCTGCGCTTGCACATGGTGTCTCACACAGGGGAGATGCCCTACAAGTGTT
  CCTCCTGCTCCCAGCAGTTCATGCAGAAGAAAGACTTGCAGAGCCACATGATCAAGCTGCACGGAGCCCC
  TAAACCCCACGCATGCCCCACTTGTGCCAAGTGCTTCCTGTCTAGGACGGAGCTACAGCTGCACGAGGCT
  TTTAAGCATCGTGGAGAAAAGCTCTTTGTGTGTGAGGAATGCGGGCACCGGGCCTCGAGCCGCAACGGAC
  TGCAGATGCACATCAAGGCCAAGCACAGGAATGAAAGGCCTTATGTCTGTGAGTTCTGCAGCCATGCCTT
  CACCCAGAAGGCCAACCTCAACATGCACCTGCGCACACACACCGGCGAGAAGCCTTTCCAGTGCCACCTC
  TGTGGGAAGACCTTCCGCACCCAAGCCAGTTTGGACAAGCACAACCGCACCCACACGGGCGAGAGGCCTT
  TCAGCTGTGAATTCTGTGAGCAGCGCTTCACTGAGAAGGGCCCCCTTCTGAGGCATGTGGCCAGCCGCCA
  TCAAGAGGGCAGGCCCCACTTCTGCCAGATCTGTGGCAAGACCTTCAAAGCTGTGGAACAGTTACGAGTG
  CACGTCAGGCGACACAAAGGTGTGAGAAAGTTCGAGTGCACCGAGTGTGGCTACAAGTTCACGCGGCAGG
  CTCACCTGCGGAGGCACATGGAGATCCACGACAGAGTGGAGAACTACAATCCCCGGCAGCGCAAGCTCCG
  GAACCTCATCATTGAGGATGAGAAGATGGTGGTCGTGGCTCTCCAGCCCCCTGCAGACCTGGAGGTGGGC
  TCTGCTGAGGTCATTGTGGAGTCCCTGACCCAGGGTGGCCTTGCCTCCCAACTCCCTAGCCAGAGACTGT
  GTTCAGAGGAGAGCTTTGCTAGCCCTGGCGTCCTGGAGCCCTCTCTCATCATCACCGCTGCTGTTCCCGA
  GGACTGTGACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224885 representing NM_133879
  Red=Cloning site Green=Tags(s)
  
  MDGSFVQHSVRVLQELNKQREKGQYCDATLDVGGLVFKAHWSVLACCSHFFQRIYGDGTGGSVVLPAGFA
  EIFGLLLDFFYTGHLALTSGNRDQVLLAAKELRVPEAVELCQSFQPQTSVGQAQSGLGQPASQDVKSHLK
  EPTDLDEEEVFRTLSLASVDQEPRDTEQPQLGTPAQSTTAFLCGKLTQALKPSPSEDKESEDCKEPPRPF
  EAGGAPLQGESNEWEVVVQVEDDRDGDYVSEPETVLTRRKSKVIRKPCAAEPALGAGSLTAEPTDSRKGA
  AVPVECPTCHKKFLSKYYLKVHNRKHTGEKPFECPKCGKCYFRKENLLEHEARNCMNRSEQVFTCSVCQE
  TFRRRMELRLHMVSHTGEMPYKCSSCSQQFMQKKDLQSHMIKLHGAPKPHACPTCAKCFLSRTELQLHEA
  FKHRGEKLFVCEECGHRASSRNGLQMHIKAKHRNERPYVCEFCSHAFTQKANLNMHLRTHTGEKPFQCHL
  CGKTFRTQASLDKHNRTHTGERPFSCEFCEQRFTEKGPLLRHVASRHQEGRPHFCQICGKTFKAVEQLRV
  HVRRHKGVRKFECTECGYKFTRQAHLRRHMEIHDRVENYNPRQRKLRNLIIEDEKMVVVALQPPADLEVG
  SAEVIVESLTQGGLASQLPSQRLCSEESFASPGVLEPSLIITAAVPEDCDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133879
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133879.3
• RefSeq Size: 2226 bp
• RefSeq ORF: 2046 bp
• Locus ID: 100090
• UniProt ID: Q1H9T6
• Cytogenetics: 4 E2
• MW: 77.3 kDa
• Gene Summary: Telomere-binding protein that acts as a regulator of telomere length. Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres. Also acts as a transcription regulator that binds to promoter regions. Regulates expression of a small subset of genes, including MTFP1. Regulates expression the J and/or S elements in MHC II promoter. Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A.[UniProtKB/Swiss-Prot Function]