Zbtb48 (NM_133879) Mouse Tagged ORF Clone

TrueORF^®
TrueORF^®

Expression-ready ORF plasmid with C-terminal tag(s)

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SKU

MR224885

Zbtb48 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48)

Datasheet SDS Plasmid Map

Reconstitution Protocol

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NM_133879 in other vectors (4)

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$686.00

4 Weeks*

Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S	DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags	30 ul	$200.00
CC100003	DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)	5 x 200 ul	$160.00
PRIMER	Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each	100 pmol	$59.00
PS100001	pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug	10 ug	$650.00
AR100028M	DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL	5 ml	$720.00

Specifications

Specifications
Product Data
Type	Mouse Tagged ORF Clone
Target Symbol	Zbtb48
Synonyms	0610011D15Rik; AI327031; Hkr3; TZAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence >MR224885 representing NM_133879 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGTTCCTTCGTGCAGCACAGCGTGAGGGTTCTGCAGGAACTCAACAAGCAGCGCGAGAAGGGCC AGTATTGCGACGCCACACTGGACGTGGGGGGCCTGGTGTTCAAGGCGCACTGGAGTGTCCTGGCGTGTTG CAGCCATTTCTTCCAGAGAATCTACGGGGATGGCACAGGGGGCAGCGTTGTCCTCCCAGCGGGCTTTGCT GAGATTTTTGGCCTCCTGCTGGACTTTTTCTACACTGGCCACCTCGCCCTCACCTCAGGGAACAGGGATC AGGTACTCTTGGCAGCCAAAGAGTTGAGGGTGCCTGAGGCTGTGGAGCTATGTCAGAGCTTCCAGCCCCA AACCTCAGTGGGACAGGCACAGAGTGGACTGGGTCAACCTGCCTCCCAGGACGTGAAAAGCCACCTGAAG GAGCCGACAGACTTGGATGAGGAGGAAGTTTTCAGGACTCTGAGCCTGGCCTCCGTGGATCAGGAGCCCA GAGACACTGAGCAGCCCCAGCTGGGTACACCTGCTCAGAGCACCACGGCCTTCCTCTGTGGGAAACTCAC GCAGGCTTTGAAGCCTTCTCCCTCAGAGGACAAAGAGTCTGAGGACTGCAAAGAGCCCCCAAGGCCCTTC GAGGCTGGAGGTGCCCCGCTGCAGGGCGAGAGCAATGAGTGGGAAGTGGTGGTCCAAGTGGAGGACGACA GGGACGGTGATTACGTATCTGAGCCTGAGACTGTGCTAACCAGAAGGAAGTCAAAAGTGATTAGAAAACC CTGTGCAGCCGAGCCAGCCCTGGGTGCGGGTTCTCTCACAGCCGAGCCCACTGACAGCAGAAAAGGTGCA GCCGTTCCGGTCGAATGTCCCACATGTCACAAAAAGTTCCTCAGCAAATATTACCTGAAAGTCCACAACA GGAAACACACTGGGGAGAAACCCTTCGAGTGTCCCAAATGTGGGAAGTGTTACTTCCGCAAGGAGAATCT CTTGGAACATGAAGCCAGGAATTGCATGAACCGCTCAGAACAGGTCTTCACATGCTCCGTATGCCAGGAG ACCTTCCGACGGAGGATGGAGCTGCGCTTGCACATGGTGTCTCACACAGGGGAGATGCCCTACAAGTGTT CCTCCTGCTCCCAGCAGTTCATGCAGAAGAAAGACTTGCAGAGCCACATGATCAAGCTGCACGGAGCCCC TAAACCCCACGCATGCCCCACTTGTGCCAAGTGCTTCCTGTCTAGGACGGAGCTACAGCTGCACGAGGCT TTTAAGCATCGTGGAGAAAAGCTCTTTGTGTGTGAGGAATGCGGGCACCGGGCCTCGAGCCGCAACGGAC TGCAGATGCACATCAAGGCCAAGCACAGGAATGAAAGGCCTTATGTCTGTGAGTTCTGCAGCCATGCCTT CACCCAGAAGGCCAACCTCAACATGCACCTGCGCACACACACCGGCGAGAAGCCTTTCCAGTGCCACCTC TGTGGGAAGACCTTCCGCACCCAAGCCAGTTTGGACAAGCACAACCGCACCCACACGGGCGAGAGGCCTT TCAGCTGTGAATTCTGTGAGCAGCGCTTCACTGAGAAGGGCCCCCTTCTGAGGCATGTGGCCAGCCGCCA TCAAGAGGGCAGGCCCCACTTCTGCCAGATCTGTGGCAAGACCTTCAAAGCTGTGGAACAGTTACGAGTG CACGTCAGGCGACACAAAGGTGTGAGAAAGTTCGAGTGCACCGAGTGTGGCTACAAGTTCACGCGGCAGG CTCACCTGCGGAGGCACATGGAGATCCACGACAGAGTGGAGAACTACAATCCCCGGCAGCGCAAGCTCCG GAACCTCATCATTGAGGATGAGAAGATGGTGGTCGTGGCTCTCCAGCCCCCTGCAGACCTGGAGGTGGGC TCTGCTGAGGTCATTGTGGAGTCCCTGACCCAGGGTGGCCTTGCCTCCCAACTCCCTAGCCAGAGACTGT GTTCAGAGGAGAGCTTTGCTAGCCCTGGCGTCCTGGAGCCCTCTCTCATCATCACCGCTGCTGTTCCCGA GGACTGTGACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR224885 representing NM_133879 Red=Cloning site Green=Tags(s) MDGSFVQHSVRVLQELNKQREKGQYCDATLDVGGLVFKAHWSVLACCSHFFQRIYGDGTGGSVVLPAGFA EIFGLLLDFFYTGHLALTSGNRDQVLLAAKELRVPEAVELCQSFQPQTSVGQAQSGLGQPASQDVKSHLK EPTDLDEEEVFRTLSLASVDQEPRDTEQPQLGTPAQSTTAFLCGKLTQALKPSPSEDKESEDCKEPPRPF EAGGAPLQGESNEWEVVVQVEDDRDGDYVSEPETVLTRRKSKVIRKPCAAEPALGAGSLTAEPTDSRKGA AVPVECPTCHKKFLSKYYLKVHNRKHTGEKPFECPKCGKCYFRKENLLEHEARNCMNRSEQVFTCSVCQE TFRRRMELRLHMVSHTGEMPYKCSSCSQQFMQKKDLQSHMIKLHGAPKPHACPTCAKCFLSRTELQLHEA FKHRGEKLFVCEECGHRASSRNGLQMHIKAKHRNERPYVCEFCSHAFTQKANLNMHLRTHTGEKPFQCHL CGKTFRTQASLDKHNRTHTGERPFSCEFCEQRFTEKGPLLRHVASRHQEGRPHFCQICGKTFKAVEQLRV HVRRHKGVRKFECTECGYKFTRQAHLRRHMEIHDRVENYNPRQRKLRNLIIEDEKMVVVALQPPADLEVG SAEVIVESLTQGGLASQLPSQRLCSEESFASPGVLEPSLIITAAVPEDCDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_133879
ORF Size	2043 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping	Ambient
Reference Data
RefSeq	NM_133879.3
RefSeq Size	2226 bp
RefSeq ORF	2046 bp
Locus ID	100090
UniProt ID	Q1H9T6
Cytogenetics	4 E2
MW	77.3 kDa
Summary	Telomere-binding protein that acts as a regulator of telomere length. Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres. Also acts as a transcription regulator that binds to promoter regions. Regulates expression of a small subset of genes, including MTFP1. Regulates expression the J and/or S elements in MHC II promoter. Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A.[UniProtKB/Swiss-Prot Function]

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Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
SDS for MR224885

Resources

cDNA Clone Resources
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety Lentivirus and AAV FAQ Virus-Mediated Gene Delivery

"NM_133879" in other vectors (4)

SKU	Description	Size	Price
MC220449	Zbtb48 (untagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48), (10ug)	10 ug	$687.00
MG224885	Zbtb48 (tGFP-tagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48), (10ug)	10 ug	$886.00
MR224885L3	Lenti ORF clone of Zbtb48 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48)	10 ug	$986.00
MR224885L4	Lenti ORF clone of Zbtb48 (mGFP-tagged) - Mouse zinc finger and BTB domain containing 48 (Zbtb48)	10 ug	$986.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.