Acot1 (NM_012006) Mouse Tagged ORF Clone

USD 440.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Acot1 (NM_012006) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Acot1
Synonyms ACH2; CTE-1; CTE-I; Cte1; D12Ucla1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR224801 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGACGCTGAACCTTGAGCCCTCGGGCCGCAGCTGCTGGGACGAACCGCTGAGCATCGCCGTGC
GCGGCCTGGCCCCGGAGCAGCCCGTCACGCTGCGCTCTGTCCTGCGCGACGAGAAGGGCGCGCTCTTCCG
AGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGCACGCCCGCGCTGGGCGGC
AGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATCGGCCTTTTTGGCGATTGG
TCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGACGGCGG
GCAGCGGCTTGCACATGCGGTGCACGAGCGTCACTTCTTGGCTCCGGGGGTGCGGCGCGTGCCCGTACGA
GAGGGCCGGGTGCGCGCCACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTCCTGGGATCATAGACCTTT
TTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTCATGGC
TCTGGCTTATTACAACTACGATGACCTCCCCAAGAACATGGAAACCATGCACATGGAGTACTTTGAAGAA
GCCGTGAACTACCTGCGCAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGCTGCTTGGGATTTCCAAAG
GGGGTGAACTTGGCCTTGCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGCTGTGGTCATCAATGGCTC
CGTGGCTGCTGTTGGGAACACCATCTCCTACAAGGATGAGACTATACCCCCTGTGACTATCCTGAGAAAT
CAGGTCAAAATGACCAAGGATGGCCTCAAGGATGTTGTAGATGCTTTGCAAAGCCCTCTGGTAGACAAGA
AGAGCTTCATTCCCGTGGAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGGTCAGGACGACCACAACTG
GAAGAGCGAGTTCTATGCTGATGAGATCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCCCAGATC
ATCTGCTACCCAGCAGCAGGGCATTACATCGAGCCCCCTTACTTCCCGCTGTGCAGCGCTGGCATGCACC
TCCTGGTGGGTGCCAACATCACCTTTGGAGGGGAGCCCAAGCCTCATGCCATGGCCCAGTTGGATGCATG
GCAGCAGCTCCAGACTTTCTTCCACAAACAGTTGGGTAGTGAGTGTTTGCATGTGTCTCCTAAAATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR224801 protein sequence
Red=Cloning site Green=Tags(s)

MEATLNLEPSGRSCWDEPLSIAVRGLAPEQPVTLRSVLRDEKGALFRAHARYRADSHGELDLARTPALGG
SFSGLEPMGLLWAMEPDRPFWRLVKRDVQTPFVVELEVLDGHEPDGGQRLAHAVHERHFLAPGVRRVPVR
EGRVRATLFLPPEPGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKNMETMHMEYFEE
AVNYLRSHPEVKGPGIGLLGISKGGELGLAMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVTILRN
QVKMTKDGLKDVVDALQSPLVDKKSFIPVERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQI
ICYPAAGHYIEPPYFPLCSAGMHLLVGANITFGGEPKPHAMAQLDAWQQLQTFFHKQLGSECLHVSPKI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_012006
ORF Size 1260 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012006.1, NM_012006.2, NP_036136.1
RefSeq Size 1559 bp
RefSeq ORF 1260 bp
Locus ID 26897
Cytogenetics 12 39.0 cM
MW 46.1 kDa

Citations (1)

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