Acot1 (NM_012006) Mouse Tagged ORF Clone

CAT#: MR224801

Acot1 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 1 (Acot1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acot1
• Synonyms: ACH2; CTE-1; CTE-I; Cte1; D12Ucla1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224801 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGACGCTGAACCTTGAGCCCTCGGGCCGCAGCTGCTGGGACGAACCGCTGAGCATCGCCGTGC
  GCGGCCTGGCCCCGGAGCAGCCCGTCACGCTGCGCTCTGTCCTGCGCGACGAGAAGGGCGCGCTCTTCCG
  AGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGCACGCCCGCGCTGGGCGGC
  AGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATCGGCCTTTTTGGCGATTGG
  TCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGACGGCGG
  GCAGCGGCTTGCACATGCGGTGCACGAGCGTCACTTCTTGGCTCCGGGGGTGCGGCGCGTGCCCGTACGA
  GAGGGCCGGGTGCGCGCCACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTCCTGGGATCATAGACCTTT
  TTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTCATGGC
  TCTGGCTTATTACAACTACGATGACCTCCCCAAGAACATGGAAACCATGCACATGGAGTACTTTGAAGAA
  GCCGTGAACTACCTGCGCAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGCTGCTTGGGATTTCCAAAG
  GGGGTGAACTTGGCCTTGCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGCTGTGGTCATCAATGGCTC
  CGTGGCTGCTGTTGGGAACACCATCTCCTACAAGGATGAGACTATACCCCCTGTGACTATCCTGAGAAAT
  CAGGTCAAAATGACCAAGGATGGCCTCAAGGATGTTGTAGATGCTTTGCAAAGCCCTCTGGTAGACAAGA
  AGAGCTTCATTCCCGTGGAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGGTCAGGACGACCACAACTG
  GAAGAGCGAGTTCTATGCTGATGAGATCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCCCAGATC
  ATCTGCTACCCAGCAGCAGGGCATTACATCGAGCCCCCTTACTTCCCGCTGTGCAGCGCTGGCATGCACC
  TCCTGGTGGGTGCCAACATCACCTTTGGAGGGGAGCCCAAGCCTCATGCCATGGCCCAGTTGGATGCATG
  GCAGCAGCTCCAGACTTTCTTCCACAAACAGTTGGGTAGTGAGTGTTTGCATGTGTCTCCTAAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224801 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEATLNLEPSGRSCWDEPLSIAVRGLAPEQPVTLRSVLRDEKGALFRAHARYRADSHGELDLARTPALGG
  SFSGLEPMGLLWAMEPDRPFWRLVKRDVQTPFVVELEVLDGHEPDGGQRLAHAVHERHFLAPGVRRVPVR
  EGRVRATLFLPPEPGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKNMETMHMEYFEE
  AVNYLRSHPEVKGPGIGLLGISKGGELGLAMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVTILRN
  QVKMTKDGLKDVVDALQSPLVDKKSFIPVERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQI
  ICYPAAGHYIEPPYFPLCSAGMHLLVGANITFGGEPKPHAMAQLDAWQQLQTFFHKQLGSECLHVSPKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012006
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012006.2, NP_036136.1
• RefSeq Size: 1559 bp
• RefSeq ORF: 1260 bp
• Locus ID: 26897
• UniProt ID: O55137
• Cytogenetics: 12 39.0 cM
• MW: 46.1 kDa
• Gene Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).[UniProtKB/Swiss-Prot Function]