Tbc1d24 (NM_001163849) Mouse Tagged ORF Clone

CAT#: MR224767

Tbc1d24 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 24 (Tbc1d24), transcript variant 3

Product Images

Circular map for MR224767

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbc1d24
• Synonyms: 9630033P11; C530046L02Rik; mKIAA1171
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224767 representing NM_001163849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCCCAGGGTACAATTGCTTTGTGGATAAGGACAAGATGGATGCATCCATCCAGGACCTGGGGC
  CAAAGGAGCTGAACTGCACGGAGCTACAGGAGCTGAAGCAACTGGCACGGCAGGGTTACTGGGCTCAGAG
  CCACACCCTGCGCGGGAAAGTGTACCAGCGCCTGATCCGGGACATCCCCTGCCGCACAGTCACACCTGAT
  GCCAGCGTGTACAGTGACATTGTGGGGAAGATTGTGGGCAAGCACAGCAGCAGTAGTCTGCCCTTGCCTG
  AGTTTGTAGACAACACTCAGGTGCCCACCTACTGCCTGAACACACGGGGTGAAGGGGCTGTGCGCAAGAT
  CCTCCTGTGTATTGCCAACCAGTTCCCTGACATCTCCTTCTGCCCTGCCCTGCCTGCTGTGGTGGCCTTG
  CTACTGCACTACAGCATCGATGAAGCTGAGTGTTTCGAAAAAGCCTGCCGCATCTTATCCTGCAATGACC
  CCACCAAGAAGCTCATTGACCAGAGCTTCCTGGCCTTTGAGTCTTCCTGTATGACATTTGGGGACCTGGT
  GAACAAGTACTGCCAGGCAGCCCATAAACTGATGGTGGCCGTGTCAGAGGACGTCCTGCAGGTCTACTCT
  GACTGGCAGCGATGGCTCTTTGGGGAGCTGCCCCTCAATTACTTTGCCCGTGTTTTTGATGTCTTCCTTG
  TAGAAGGCTACAAAGTATTGTACCGAGTTGCTCTGGCCATCCTCAAGTTCTTCCACAAGGTGAGAGCAGG
  ACAGCCCCTTGAGTCAGACAATGTAAAGCAAGACATCCGAATGTTTGTCAAGGACATTGCCAAGACAGTG
  TCCCCTGAGAAACTACTAGAGAAGGCCTTTGCCATCCGCCTATTTTCCCGAAAGGAGATCCAGCTCTTGC
  AAATGGCCAATGAGAAAGCACTGAGGCAGAAGGGTATAACTGTCAAACAGAAGAGTGTCTCACTTTCTAA
  AAGGCAGTTTGTGCACTTAGCTGTCCATGCAGAGAACTTCCACTCAGAGATTGTCAGCGTGAAGGAGATG
  AGAGACATTTGGTCCTGGATCCCTGAGCGCTTTGCCCTCTGCCAACCCCTCCTGCTCTTCTCATCACTGC
  AGCATGGGTACAGCCTAAGCAGGTTCTATTTCCAGTGTGAAGGACATGAGCCCACCCTCCTGCTCATCAA
  GACCACTCAAAAGGAGGTCTGTGGGGCTTACCTGTCAACAGACTGGAGCGAGAGGACTAAATTCGGAGGC
  AAGCTGGGCTTCTTTGGGACTGGAGAATGCTTTGTTTTTAGGCTGCAGCCGGAAGTGCAGCGCTATGAGT
  GGGTGGTCATCAAACACCCAGAGCTGACCAAGGCAACATCCCTCAAGTCTTCAGAGGCTGCAGGCAGTTC
  TTCCCTCATCAGCCACTGCTCTTCAGACCCCGCTGATCGGCTCTCCCCTTTCCTGGCTGCCCGGCACTTC
  AACCTGCCCTCCAAGACTGAGTCCATGTTCATGGCTGGAGGCAATGACTGCCTCATCATTGGGGGAGGAG
  GTGGTCAAGCACTCTACGTTGATGGGGACCTGAATCGAGGGCGCACTGGACACTGTGACACATTCAACAA
  CCAGCCCCTCTGCTCTGAGAACTTCCTCATCGCAGCTGTGGAGGCTTGGGGCTTCCAAGACCCTGACACC
  GAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224767 representing NM_001163849
  Red=Cloning site Green=Tags(s)
  
  MDPPGYNCFVDKDKMDASIQDLGPKELNCTELQELKQLARQGYWAQSHTLRGKVYQRLIRDIPCRTVTPD
  ASVYSDIVGKIVGKHSSSSLPLPEFVDNTQVPTYCLNTRGEGAVRKILLCIANQFPDISFCPALPAVVAL
  LLHYSIDEAECFEKACRILSCNDPTKKLIDQSFLAFESSCMTFGDLVNKYCQAAHKLMVAVSEDVLQVYS
  DWQRWLFGELPLNYFARVFDVFLVEGYKVLYRVALAILKFFHKVRAGQPLESDNVKQDIRMFVKDIAKTV
  SPEKLLEKAFAIRLFSRKEIQLLQMANEKALRQKGITVKQKSVSLSKRQFVHLAVHAENFHSEIVSVKEM
  RDIWSWIPERFALCQPLLLFSSLQHGYSLSRFYFQCEGHEPTLLLIKTTQKEVCGAYLSTDWSERTKFGG
  KLGFFGTGECFVFRLQPEVQRYEWVVIKHPELTKATSLKSSEAAGSSSLISHCSSDPADRLSPFLAARHF
  NLPSKTESMFMAGGNDCLIIGGGGGQALYVDGDLNRGRTGHCDTFNNQPLCSENFLIAAVEAWGFQDPDT
  E
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163849
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163849.1, NP_001157321.1
• RefSeq Size: 7180 bp
• RefSeq ORF: 1686 bp
• Locus ID: 224617
• UniProt ID: Q3UUG6
• Cytogenetics: 17 A3.3
• MW: 63.7 kDa
• Gene Summary: May act as a GTPase-activating protein for Rab family protein(s) (PubMed:20727515). Involved in neuronal projections development, probably through a negative modulation of ARF6 function (PubMed:20727515).[UniProtKB/Swiss-Prot Function]