Elf3 (NM_007921) Mouse Tagged ORF Clone

CAT#: MR224439

Elf3 (Myc-DDK-tagged) - Mouse E74-like factor 3 (Elf3), transcript variant 2

Product Images

Circular map for MR224439

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elf3
• Synonyms: ESE-1; ESX; jen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224439 representing NM_007921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCACCTGTGAGATCAGCAACGTTTTTAGTAACTACTTCAACGCCATGTACAGCTCAGAAGACC
  CCACCCTGGCTCCTGCTCCTCCGACTACCTTTGGCACTGAAGACTTGGTGTTGACCCTGAACAACCAACA
  GATGACACTGGAAGGTCCAGAGAAGGCAAGCTGGACTAGCGAGCGGCCCCAGTTCTGGTCGAAGACCCAG
  GTTCTGGAGTGGATCAGCTACCAAGTGGAGAAGAACAAGTATGACGCCAGCTCCATCGACTTCTCCCGCT
  GCGACATGGACGGAGCCACCCTCTGCAGCTGTGCGCTGGAGGAGCTGCGGCTAGTCTTTGGACCTCTGGG
  AGACCAGCTCCATGCCCAGCTTCGGGACCTCACCTCCAACTCTTCTGATGAACTCAGCTGGATCATCGAG
  CTGCTGGAGAAGGATGGCATGTCCTTCCAAGAGAGCCTAGGCGACTCGGGCCCCTTTGATCAGGGAAGTC
  CTTTTGCCCAGGAACTCCTGGATGATGGCCGCCAGGCCAGTCCCTACTACTGCAGTACCTATGGCCCTGG
  AGCGCCCTCCCCCGGCAGCTCTGATGTCTCCACTGCAAGGACCGCTACTCCCCAGAGTTCCCATGCTTCT
  GACTCCGGTGGAAGTGATGTGGACCTGGACCTCACCGAGAGCAAGGTCTTCCCTAGAGATGGCTTTCCTG
  ACTATAAGAAGGGGGAACCCAAGCACGGGAAGAGGAAACGGGGGCGTCCCAGAAAGCTGAGCAAGGAATA
  CTGGGACTGTCTGGAGGGCAAGAAGAGCAAGCACGCCCCCAGAGGTACTCACCTGTGGGAGTTTATCCGA
  GACATCCTAATCCACCCCGAGCTCAACGAAGGCCTCATGAAGTGGGAGAACCGGCACGAGGGTGTGTTCA
  AGTTTCTTCGCTCAGAGGCCGTGGCCCAACTCTGGGGCCAGAAGAAGAAGAACAGCAACATGACCTATGA
  GAAGCTGAGCCGAGCCATGAGGTATTACTACAAACGGGAGATCCTGGAACGGGTGGATGGCCGACGGCTC
  GTCTACAAGTTTGGCAAGAACTCTAGTGGCTGGAAGGAAGAAGAGGTTGGAGAGAGTCGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224439 representing NM_007921
  Red=Cloning site Green=Tags(s)
  
  MAATCEISNVFSNYFNAMYSSEDPTLAPAPPTTFGTEDLVLTLNNQQMTLEGPEKASWTSERPQFWSKTQ
  VLEWISYQVEKNKYDASSIDFSRCDMDGATLCSCALEELRLVFGPLGDQLHAQLRDLTSNSSDELSWIIE
  LLEKDGMSFQESLGDSGPFDQGSPFAQELLDDGRQASPYYCSTYGPGAPSPGSSDVSTARTATPQSSHAS
  DSGGSDVDLDLTESKVFPRDGFPDYKKGEPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIR
  DILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRL
  VYKFGKNSSGWKEEEVGESRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007921
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007921.3, NP_031947.1
• RefSeq Size: 1940 bp
• RefSeq ORF: 1116 bp
• Locus ID: 13710
• UniProt ID: Q3UPW2
• Cytogenetics: 1 E4
• MW: 42.5 kDa
• Gene Summary: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter (By similarity). Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity (By similarity). Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway (By similarity). May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.[UniProtKB/Swiss-Prot Function]