Lgi2 (NM_144945) Mouse Tagged ORF Clone

CAT#: MR224432

Lgi2 (Myc-DDK-tagged) - Mouse leucine-rich repeat LGI family, member 2 (Lgi2)

Product Images

Circular map for MR224432

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lgi2
• Synonyms: mKIAA1916
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224432 representing NM_144945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTATGGAGAGGCGGCGGGGCGCTGGGGTTGCTGCTGCTGAGCGCCGCGTGCCTCATCCCGCCGA
  GCGCGCAGGTGAGGCGGTTGGCGCGCTGCCCCGCCACTTGCAGCTGTACCAAGGAGTCCATCATCTGCGT
  GGGCTCCTCTTGGGTGCCTAGGATCGTGCCTGGCGACATCAGCTCCCTGAGCCTAGTAAATGGAACATTT
  CTGGAAATCAAGGACCGAATGTTTTCCCACCTGCCCTCTCTGCAGCTGCTATTGCTGAATTCTAACTCAT
  TCACGGTGATCCGGGACGATGCCTTTGCTGGCCTTTTTCATCTTGAATACCTGTTCATTGAAGGGAACAA
  AATAGAAACCATTTCAAGAAATGCCTTTCGGGGCCTCCGTGACCTGACTCACCTAGACTTAAGGGGTAAT
  AAATTTGAATGTGACTGCAAAGCCAAATGGCTGTATCTATGGCTGAAGATGACAAACTCCACCGTCTCTG
  ATGTGCTGTGTATCGGTCCACCAGAATACCAGGAGAAGAAACTCAACGAAGTGACCAGCTTTGACTATGA
  GTGTACCACCACAGGTCCCCAGACTGATGAAGCCAAGCAGAGAGGATGGCAGTTGGAACTCTCCCTGGGA
  TTTTGTGAACTAATATTTGTTTTTCAACACCCACTCTCAGATTTTGTCGTTCATCAGACTCTGCCGTACC
  AGTCGGTGTCAGTAGACACGTTCAACTCCAAGAACGATGTGTACGTGGCCATCGCTCAGCCCAGCATGGA
  GAACTGCATGGTGTTGGAGTGGGACCACATAGAAATGAATTTCCGGAGTTATGACAATATCACAGGCCAG
  TCCATCGTGGGCTGCAAGGCCATCCTCATTGACGACCAGGTCTTTGTGGTGGTGGCCCAGCTCTTCGGTG
  GCTCTCACATTTACAAATACGACGAGAGCTGGACCAAGTTTGTCAAGTTCCAAGACATAGAGGTGTCTCG
  GATTTCCAAGCCCAACGACATTGAGCTGTTCGAGATCGACGACGAGACCTTCTTCATCATCGCCGACAGC
  TCCAAAGCGGGGCTGTCCACAGTTTACAAGTGGAACAGCAAAGGGTTTTACTCCTACCAGTCACTACATG
  AGTGGTTCAGGGACACAGATGCGGAGTTTGTTGACATCGACGGGAAGTCTCACCTCATCCTGTCTAGCCG
  CTCCCAGGTCCCCATCATCCTGCAGTGGAATAAAAGTTCCAAGAAGTTTGTCCCCCATGGTGACATCCCC
  AATATGGAGGATGTGCTGGCTGTGAAGAGCTTCCGGATGCAGAACACCCTCTACCTTTCCCTCACCCGTT
  TCATTGGGGACTCGCGGGTCATGCGTTGGAACAGTAAGCAGTTTGTGGAGGTCCAGGCTCTTCCTTCCCG
  GGGGGCTATGACCTTGCAACCCTTCTCCTTCAAAGATAACCACTACCTGGCCCTGGGGAGTGACTACACG
  TTCTCACAGATCTACCAGTGGGATAAAGAGAAACAGCAGTTTAAAAAGTTTAAAGAAATCTATGTGCAGG
  CGCCTCGGTCCTTCACAGCCGTCTCCACCGACAGGAGAGATTTCTTTTTTGCATCCAGTTTCAAAGGGAA
  GACAAAGATTTTTGAACATATCATTGTTGATTTAAGTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224432 representing NM_144945
  Red=Cloning site Green=Tags(s)
  
  MALWRGGGALGLLLLSAACLIPPSAQVRRLARCPATCSCTKESIICVGSSWVPRIVPGDISSLSLVNGTF
  LEIKDRMFSHLPSLQLLLLNSNSFTVIRDDAFAGLFHLEYLFIEGNKIETISRNAFRGLRDLTHLDLRGN
  KFECDCKAKWLYLWLKMTNSTVSDVLCIGPPEYQEKKLNEVTSFDYECTTTGPQTDEAKQRGWQLELSLG
  FCELIFVFQHPLSDFVVHQTLPYQSVSVDTFNSKNDVYVAIAQPSMENCMVLEWDHIEMNFRSYDNITGQ
  SIVGCKAILIDDQVFVVVAQLFGGSHIYKYDESWTKFVKFQDIEVSRISKPNDIELFEIDDETFFIIADS
  SKAGLSTVYKWNSKGFYSYQSLHEWFRDTDAEFVDIDGKSHLILSSRSQVPIILQWNKSSKKFVPHGDIP
  NMEDVLAVKSFRMQNTLYLSLTRFIGDSRVMRWNSKQFVEVQALPSRGAMTLQPFSFKDNHYLALGSDYT
  FSQIYQWDKEKQQFKKFKEIYVQAPRSFTAVSTDRRDFFFASSFKGKTKIFEHIIVDLSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144945
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144945.3, NP_659194.1
• RefSeq Size: 1927 bp
• RefSeq ORF: 1653 bp
• Locus ID: 246316
• UniProt ID: Q8K4Z0
• Cytogenetics: 5 C1
• MW: 63.5 kDa
• Gene Summary: Required for the development of soma-targeting inhibitory GABAergic synapses made by parvalbumin-positive basket cells.[UniProtKB/Swiss-Prot Function]