Smarcd2 (NM_001130187) Mouse Tagged ORF Clone

CAT#: MR224417

Smarcd2 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (Smarcd2), transcript variant 1

Product Images

Circular map for MR224417

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smarcd2
• Synonyms: AW322457; Baf60b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224417 representing NM_001130187
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCCGTGGCGCGGGCGGGTTCCCGCTGCCTCCGCTGAGCCCCGGCGGCGGCGCCGTTGCCGCGG
  CCCTTGGTGCGCCGCCTCCGCCTGCGGGACCCGGAATGCTGCCCAGCCCGGCGCTCAGGGGCCCGGGGCC
  TTCTGGAGGCATGGGGGTACCGGGGGCCGCCGCCTTCCGCCCCATGGGCCCCGCTGGCCCCGCGGCGCAG
  TACCAGCGTCCTGGCATGTCACCAGGAAGCAGGATGCCCATGGCTGGCTTGCAGGTGGGACCTCCTGCCG
  GTTCCCCATTTGGCACAGCTGCTCCGCTCCGACCTGGCATGCCACCTACCATGATGGATCCATTCCGAAA
  ACGCCTGCTTGTGCCTCAGGCCCAGCCCCCGATGCCTGCCCAGCGCCGAGGGTTAAAGAGGAGGAAGATG
  GCAGATAAGGTTCTACCTCAGCGAATCCGGGAGCTTGTCCCAGAGTCTCAGGCATACATGGATCTTTTAG
  CTTTCGAGAGGAAGCTGGACCAGACCATCGCTCGCAAGCGGATGGAGATTCAAGAGGCCATCAAGAAGCC
  TCTGACGCAAAAGCGAAAACTTCGGATCTATATTTCCAATACATTCAGCCCCAGCAAGGCGGATGGAGAT
  AATGCGGGAACTGCGGGGACCCCTGGGGGAACCCCGGCAGCAGACAAGGTGGCCTCCTGGGAGCTTCGAG
  TAGAGGGGAAACTGCTGGATGATCCTAGCAAACAGAAGAGGAAGTTCTCATCATTCTTTAAGAGCCTTGT
  GATTGAGTTGGACAAGGAACTCTATGGGCCGGACAACCATCTGGTGGAGTGGCATCGGATGCCCACCACA
  CAGGAAACAGATGGCTTTCAGGTGAAACGGCCAGGAGATCTCAATGTCAAGTGCACCCTTCTGCTCATGC
  TGGATCATCAGCCTCCTCAGTATAAACTGGACCCCCGCCTGGCGAGGTTGCTGGGAGTGCACACACAGAC
  CAGGGCGGCAATCATGCAGGCACTGTGGCTTTACATCAAACACAACCAGCTGCAGGACGGCCATGAGCGC
  GAGTACATCAACTGCAATCGTTACTTCCGCCAGATCTTCAGTTGTGGCCGACTCCGTTTCTCCGAGATTC
  CCATGAAGCTGGCTGGATTGCTGCAGCATCCAGACCCCATTGTTATTAATCATGTCATTAGTGTGGATCC
  TAATGACCAAAAGAAGACAGCCTGCTATGACATTGATGTAGAGGTTGATGACCCACTGAAGGCCCAGATG
  AGCAACTTCCTGGCCTCTACCACCAACCAGCAGGAGATTGCTTCTCTTGACGTCAAGATCCATGAGACCA
  TTGAGTCCATCAACCAGCTAAAGACCCAGAGGGATTTCATGCTCAGCTTTAGCACCGAGCCCCAGGACTT
  CATCCAGGAGTGGCTCCGTTCCCAACGCCGAGACCTCAAGATCATCACAGATGTGATTGGAAACCCTGAG
  GAGGAGAGACGAGCTGCTTTCTACCACCAGCCCTGGGCTCAGGAAGCAGTGGGGAGGCACATCTTTGCCA
  AGGTGCAGCAGCGAAGGCAGGAACTGGAACAGGTGCTGGGAATTCGCCTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224417 representing NM_001130187
  Red=Cloning site Green=Tags(s)
  
  MSGRGAGGFPLPPLSPGGGAVAAALGAPPPPAGPGMLPSPALRGPGPSGGMGVPGAAAFRPMGPAGPAAQ
  YQRPGMSPGSRMPMAGLQVGPPAGSPFGTAAPLRPGMPPTMMDPFRKRLLVPQAQPPMPAQRRGLKRRKM
  ADKVLPQRIRELVPESQAYMDLLAFERKLDQTIARKRMEIQEAIKKPLTQKRKLRIYISNTFSPSKADGD
  NAGTAGTPGGTPAADKVASWELRVEGKLLDDPSKQKRKFSSFFKSLVIELDKELYGPDNHLVEWHRMPTT
  QETDGFQVKRPGDLNVKCTLLLMLDHQPPQYKLDPRLARLLGVHTQTRAAIMQALWLYIKHNQLQDGHER
  EYINCNRYFRQIFSCGRLRFSEIPMKLAGLLQHPDPIVINHVISVDPNDQKKTACYDIDVEVDDPLKAQM
  SNFLASTTNQQEIASLDVKIHETIESINQLKTQRDFMLSFSTEPQDFIQEWLRSQRRDLKIITDVIGNPE
  EERRAAFYHQPWAQEAVGRHIFAKVQQRRQELEQVLGIRLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130187
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130187.1, NP_001123659.1
• RefSeq Size: 2767 bp
• RefSeq ORF: 1596 bp
• Locus ID: 83796
• UniProt ID: Q99JR8
• Cytogenetics: 11 E1
• MW: 59.5 kDa
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation.[UniProtKB/Swiss-Prot Function]