Slc25a44 (NM_178696) Mouse Tagged ORF Clone

CAT#: MR224346

Slc25a44 (Myc-DDK-tagged) - Mouse solute carrier family 25, member 44 (Slc25a44), transcript variant 1

Product Images

Circular map for MR224346

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc25a44
• Synonyms: 6720482A19; B430110G05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224346 representing NM_178696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGGGACTCTGAATTGGCCGTGCAAGAGCCTGACAGAAGGTCTCCGGGCACAATGGAGGACAAAC
  GGAACATCCAGATCATCGAGTGGGAGCACCTGGACAAGAAGAAGTTCTATGTGTTTGGTGTTGCTATGAC
  AATGATGATCAGGGTCAGCGTGTACCCGTTCACCCTCATCCGCACCCGCCTGCAGGTCCAGAAGGGCAAG
  AGCCTCTACCACGGGACCTTCGATGCCTTTGTCAAGATCCTGCGAGCAGATGGAGTGGCGGGCCTCTACC
  GGGGGTTCCTGGTCAACACCTTCACACTCATCTCCGGCCAGTGCTATGTCACCACTTACGAGCTCACCCG
  GAAGTTTGTGGCAGACTACAGCCAGAGTAACACAGTCAAATCACTCGTAGCCGGGGGCTCGGCCTCCCTT
  GTGGCCCAGAGCATCACGGTGCCGATCGATGTTGTCTCCCAACATCTGATGATGCAGCGGAAGGGTGAGA
  AAATGGGTCGCTTCCAAGTGCACGGGAACCTAGAGGGACAAGGGGTGATTGCCTTTGGCCAGACTAAGGA
  CATCATCAGGCAGATCCTTCGGGCTGACGGTCTCCGAGGCTTCTACCGGGGCTATGTGGCCTCGCTGCTA
  ACGTACATCCCAAACAGTGCTGTCTGGTGGCCCTTCTACCACTTCTATGCAGAGCAGCTGTCGCGCCTGT
  GCCCTCAGGAGTGCCCTCACATTGTCTTTCAAGCCATCTCTGGGCCCCTGGCTGCAGCCACTGCCTCCAT
  CCTCACCAATCCCATGGATGTCATCAGAACCCGTGTGCAGGTTGAAGGCAAGAGCTCCATCGTCCTGACC
  TTCAGGCAGCTGATGGCAGAGGAGGGGCCTTGGGGCCTCATGAAGGGCCTCTCGGCAAGAATCATCTCGG
  CTACACCCTCCACCATCGTCATCGTGGTGGGCTACGAGAGCCTCAAGAAGCTCAGCCTGCGACCTGAGCT
  GGTGGACTCGAGACACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224346 representing NM_178696
  Red=Cloning site Green=Tags(s)
  
  MPGDSELAVQEPDRRSPGTMEDKRNIQIIEWEHLDKKKFYVFGVAMTMMIRVSVYPFTLIRTRLQVQKGK
  SLYHGTFDAFVKILRADGVAGLYRGFLVNTFTLISGQCYVTTYELTRKFVADYSQSNTVKSLVAGGSASL
  VAQSITVPIDVVSQHLMMQRKGEKMGRFQVHGNLEGQGVIAFGQTKDIIRQILRADGLRGFYRGYVASLL
  TYIPNSAVWWPFYHFYAEQLSRLCPQECPHIVFQAISGPLAAATASILTNPMDVIRTRVQVEGKSSIVLT
  FRQLMAEEGPWGLMKGLSARIISATPSTIVIVVGYESLKKLSLRPELVDSRHW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178696
• ORF Size: 999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178696.5, NP_848811.2
• RefSeq Size: 3584 bp
• RefSeq ORF: 1002 bp
• Locus ID: 229517
• Cytogenetics: 3 F1
• MW: 37.8 kDa
• Gene Summary: Mitochondrial solute transporter which transports branched-chain amino acid (BCAA; valine, leucine and isoleucine) into mitochondria in brown adipose tissue (BAT) (PubMed:31435015). BAT is involved in BCAA catabolism and actively utilizes BCAA in the mitochondria for thermogenesis (PubMed:31435015).[UniProtKB/Swiss-Prot Function]