Aifm1 (NM_012019) Mouse Tagged ORF Clone

CAT#: MR224344

Aifm1 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR224344

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aifm1
• Synonyms: AIF; AIFsh2; Hq; Pdcd8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224344 representing NM_012019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGTGTGGAGGCCTGGCGGGTGCTTTCAAGCAGAAACTGGTGCCCTTGGTGCGGACGGTGTACG
  TCCAGAGGCCGAAACAGAGGAACCGGCTTCCAGGCAACTTGTTCCAGCAATGGCGTGTTCCTCTAGAACT
  CCAGATGGCAAGACAAATGGCTAGCTCTGGTTCATCAGGGGGCAAAATGGATAATTCTGTGTTAGTCCTT
  ATTGTGGGCTTATCAACAATAGGAGCTGGTGCATATGCCTACAAAACTATAAAAGAAGACCAAAAAAGAT
  ACAATGAAAGAGTGATGGGATTAGGACTGTCCCCAGAAGAGAAACAGAGAAGAGCCATTGCCTCCGCTAC
  AGAGGGAGGCTCAGTTCCTCAGATCAGGGCACCAAGTCACGTCCCTTTCCTGCTGATTGGTGGAGGGACT
  GCTGCTTTTGCAGCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGGGTCCTGATTGTATCTGAAG
  ACCCTGAACTGCCATACATGCGACCTCCTCTTTCAAAAGAATTGTGGTTTTCAGATGATCCAAATGTCAC
  AAAGACACTGCAATTCAGACAGTGGAATGGAAAAGAGAGAAGCATATATTTCCAGCCACCTTCTTTCTAT
  GTCTCTGCTCAGGACCTGCCTAATATTGAGAACGGTGGTGTGGCTGTCCTCACTGGGAAAAAGGTAGTAC
  ATCTGGATGTAAGAGGCAACATGGTGAAACTTAATGATGGCTCTCAGATTACCTTTGAAAAGTGCTTGAT
  TGCAACGGGAGGCACTCCAAGAAGTCTGTCTGCCATCGATAGGGCTGGAGCAGAGGTGAAGAGTAGAACA
  ACACTTTTCAGGAAGATTGGAGATTTTAGAGCCTTGGAGAAGATCTCTCGGGAGGTCAAGTCAATTACAG
  TTATCGGCGGGGGCTTCCTTGGGAGTGAGCTGGCCTGTGCTCTTGGCAGAAAGTCTCAAGCCTCGGGCAT
  AGAAGTGATCCAGCTGTTCCCTGAGAAAGGAAATATGGGGAAGATCCTTCCTCAATACCTCAGCAACTGG
  ACCATGGAAAAAGTCAAACGAGAGGGAGTGAAAGTGATGCCCAATGCAATTGTACAATCAGTTGGAGTCA
  GCGGTGGCAGGTTACTCATTAAGCTGAAAGATGGAAGGAAGGTAGAAACTGACCACATAGTGACAGCTGT
  GGGCCTAGAGCCCAATGTTGAGTTGGCCAAGACTGGCGGACTGGAAATAGATTCCGATTTTGGTGGCTTC
  CGGGTAAATGCAGAACTCCAAGCACGTTCTAACATCTGGGTGGCAGGGGATGCTGCATGCTTCTATGATA
  TAAAGTTGGGTCGAAGGCGAGTAGAGCATCATGATCATGCTGTTGTGAGTGGAAGACTGGCTGGAGAAAA
  CATGACTGGAGCCGCTAAGCCATACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGTCCTGATGTCGGC
  TATGAAGCTATTGGTCTGGTGGATAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAG
  ACAACCCAAAATCTGCCACAGAGCAGTCAGGAACTGGTATCCGTTCGGAGAGTGAGACAGAGTCAGAAGC
  TTCGGAAATCACAATTCCTCCCAGCGCCCCTGCAGTCCCACAGGTCCCTGTTGAAGGGGAGGACTACGGC
  AAAGGTGTCATCTTCTACCTCAGGGACAAAGTTGTGGTGGGGATTGTGCTATGGAACGTCTTTAACCGAA
  TGCCAATTGCAAGGAAGATCATTAAGGACGGTGAGCAACATGAAGATCTCAATGAAGTAGCTAAACTCTT
  CAACATTCATGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224344 representing NM_012019
  Red=Cloning site Green=Tags(s)
  
  MFRCGGLAGAFKQKLVPLVRTVYVQRPKQRNRLPGNLFQQWRVPLELQMARQMASSGSSGGKMDNSVLVL
  IVGLSTIGAGAYAYKTIKEDQKRYNERVMGLGLSPEEKQRRAIASATEGGSVPQIRAPSHVPFLLIGGGT
  AAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLQFRQWNGKERSIYFQPPSFY
  VSAQDLPNIENGGVAVLTGKKVVHLDVRGNMVKLNDGSQITFEKCLIATGGTPRSLSAIDRAGAEVKSRT
  TLFRKIGDFRALEKISREVKSITVIGGGFLGSELACALGRKSQASGIEVIQLFPEKGNMGKILPQYLSNW
  TMEKVKREGVKVMPNAIVQSVGVSGGRLLIKLKDGRKVETDHIVTAVGLEPNVELAKTGGLEIDSDFGGF
  RVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVG
  YEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSAPAVPQVPVEGEDYG
  KGVIFYLRDKVVVGIVLWNVFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012019
• ORF Size: 1836 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012019.3, NP_036149.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1839 bp
• Locus ID: 26926
• UniProt ID: Q9Z0X1
• Cytogenetics: X 25.68 cM
• MW: 67.2 kDa
• Gene Summary: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]