Slc25a13 (NM_015829) Mouse Tagged ORF Clone

CAT#: MR224237

Slc25a13 (Myc-DDK-tagged) - Mouse solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13 (Slc25a13), nuclear gene encoding mitochondrial protein, transcrip

Product Images

Circular map for MR224237

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc25a13
• Synonyms: AI785475; Ctrn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224237 representing NM_015829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCCAAGGTGGCTTTAACCAAGAGAGCCGATCCAGCTGAGCTTAAAGCAATATTTTTGAAGT
  ATGCGAGCATTGAAAAAAATGGTGAATTTTTTATGTCTCCTCATGACTTTGTCACTCGGTATTTGAATAT
  TTTTGGAGAAAGCCAGCCCAACCCGAAAACTGTGGAACTTTTAAGTGGTGTAGTGGATCAGACCAAAGAT
  GGGTTAATATCCTTCCAAGAGTTTGTAGCCTTTGAGTCTGTCCTGTGTGCTCCGGATGCCTTGTTTATGG
  TGGCGTTCCAGCTGTTTGACAAAGCTGGCAAAGGAGAGGTTACTTTTGAGGATGTCAAGCAAATTTTTGG
  ACAGACTACAATTCATCAACATATTCCATTTAACTGGGATTCAGAATTTGTGCAGCTACATTTTGGAAAA
  GAAAGAAAAAGGCACCTTACATATGCGGAGTTCACTCAGTTTTTGTTGGAAATACAGTTGGAGCACGCGA
  AGCAAGCCTTTGTGCAACGAGACAATGCCAAGACAGGCAAAGTCTCAGCCATTGACTTCCGGGACATCAT
  GGTCACCATCCGTCCCCATGTCCTGACGCCTTTTGTTGAGGAATGTCTAGTAGCTGCTGCTGGAGGCACT
  AGGTCCCATCAAGTTAGCTTCTCCTATTTTAATGGATTTAATTCGCTCCTTAACAACATGGAACTCATTA
  GGAAGATCTACAGTACTCTGGCTGGCAACAGGAAAGACGTGGAGGTGACTAAGGAGGAGTTCGCTTTGGC
  AGCCCAGAAGTTTGGTCAGGTTACACCCATGGAAGTTGACATCTTGTTTCAGTTAGCAGATTTATATGAG
  CCGAGGGGGCGCATGACCTTAGCAGACATTGAGCGGATCGCCCCTCTAGAGGAAGGAATGTTACCCTTCA
  ACTTGGCCGAGGCCCAGAGGCAGCAGAAGGCCTCGGGTGATGCTGCTCGGCCCTTTCTTCTGCAGCTCGC
  AGAGTCAGCCTACAGGTTTGGACTGGGTTCTATTGCTGGAGCTGTCGGAGCCACTGCCGTGTATCCTATC
  GATCTTGTAAAGACTCGAATGCAGAACCAGAGATCAACCGGCTCTTTTGTGGGCGAGCTCATGTATAAAA
  ACAGCTTTGACTGCTTTAAGAAGGTGCTCCGCTACGAAGGCTTCTTTGGACTGTACCGAGGTTTGCTGCC
  ACAGTTACTGGGAGTCGCCCCGGAGAAGGCCATAAAACTTACAGTGAACGATTTTGTGAGAGATAAATTT
  ATGCACAAAGATGGCTCCGTCCCACTTTTGGCAGAGATTTTTGCTGGAGGCTGTGCTGGAGGCTCCCAAG
  TGATTTTCACAAATCCTTTAGAGATTGTGAAAATCCGCTTACAAGTGGCCGGAGAGATCACCACTGGCCC
  CCGAGTCAGCGCCCTGTCGGTGGTACGAGACCTGGGGTTCTTTGGCATCTACAAGGGCGCCAAAGCATGC
  TTCCTGAGGGACATTCCTTTCTCAGCCATCTACTTCCCGTGCTATGCCCACGTGAAGGCTTCCTTTGCAA
  ATGAAGACGGGCAGGTCAGCCCCGGAAGCCTGCTCTTAGCCGGTGCTATTGCTGGTATGCCTGCGGCATC
  TTTAGTGACCCCTGCTGATGTTATCAAGACTAGATTACAGGTGGCTGCCCGGGCTGGCCAAACCACTTAC
  AACGGTGTGACCGACTGCTTCAGAAAGATACTTCGAGAAGAGGGCCCCAAAGCATTGTGGAAAGGAGTTG
  CTGCTCGTGTCTTCCGCTCCTCGCCCCAGTTTGGTGTCACCTTGCTTACGTATGAATTGCTGCAGCGGTG
  GTTCTATGTCGATTTTGGAGGCGTGAAGCCCGTGGGATCTGAGCCGGTTCCTAAATCCCGGATTACTCTG
  CCCGCTCCCAATCCTGACCACGTAGGGGGCTACAAACTGGCCGTTGCCACATTCGCAGGGATTGAGAACA
  AATTTGGACTTTACCTACCTCTCTTTAAGCCATCAGCATCTACCTCAAAAGTGACTGCTGGGGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224237 representing NM_015829
  Red=Cloning site Green=Tags(s)
  
  MAAAKVALTKRADPAELKAIFLKYASIEKNGEFFMSPHDFVTRYLNIFGESQPNPKTVELLSGVVDQTKD
  GLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQIFGQTTIHQHIPFNWDSEFVQLHFGK
  ERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNAKTGKVSAIDFRDIMVTIRPHVLTPFVEECLVAAAGGT
  RSHQVSFSYFNGFNSLLNNMELIRKIYSTLAGNRKDVEVTKEEFALAAQKFGQVTPMEVDILFQLADLYE
  PRGRMTLADIERIAPLEEGMLPFNLAEAQRQQKASGDAARPFLLQLAESAYRFGLGSIAGAVGATAVYPI
  DLVKTRMQNQRSTGSFVGELMYKNSFDCFKKVLRYEGFFGLYRGLLPQLLGVAPEKAIKLTVNDFVRDKF
  MHKDGSVPLLAEIFAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALSVVRDLGFFGIYKGAKAC
  FLRDIPFSAIYFPCYAHVKASFANEDGQVSPGSLLLAGAIAGMPAASLVTPADVIKTRLQVAARAGQTTY
  NGVTDCFRKILREEGPKALWKGVAARVFRSSPQFGVTLLTYELLQRWFYVDFGGVKPVGSEPVPKSRITL
  PAPNPDHVGGYKLAVATFAGIENKFGLYLPLFKPSASTSKVTAGDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015829
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015829.3, NP_056644.1
• RefSeq Size: 3133 bp
• RefSeq ORF: 2031 bp
• Locus ID: 50799
• UniProt ID: Q9QXX4
• Cytogenetics: 6 A1
• MW: 74.9 kDa
• Gene Summary: Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.[UniProtKB/Swiss-Prot Function]