Cpeb4 (NM_026252) Mouse Tagged ORF Clone

CAT#: MR224198

Cpeb4 (Myc-DDK-tagged) - Mouse cytoplasmic polyadenylation element binding protein 4 (Cpeb4)

Product Images

Circular map for MR224198

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpeb4
• Synonyms: Cpe-bp4; Cpeb-4; mCPEB-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224198 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATTACGGGTTTGGAGTGCTAGTGCAAAGCAATACTGGGAATAAATCTGCTTTTCCAGTCCGAT
  TCCATCCACATCTGCAGCCTCCACACCATCACCAAAATGCCACCCCCAACCCTGCTGCTTTTATAAATAA
  TAACACAGCTGCCAATGGCAGCAGTGCTGGGTCAGCCTGGCTCTTTCCTGCCCCGGCTACTCATAACATT
  CAGGATGAGATCTTGGGGTCAGAAAAAGCAAAAAGTCAACAGCAAGAACAACAAGACCCTTTAGAAAAGC
  AACAGCTCTCCCCGAGTCCAGGTCAGGAAGCTGGAATACTGCCTGAAACTGAAAAGGCAAAAGCTGAAGA
  AAATCCAGGGGACAGTTCTTCAGAAAACAGCAACGGAAAAGAAAAACTACGCATTGAATCACCAGTGTTG
  ACAGGGTTTGATTACCAAGAAGCCACCGGTCTCGGGACTTCCACCCAACCCTTGACATCCAGTGCATCGT
  CCCTTACTGGTTTCAGTAACTGGTCAGCAGCGATAGCACCTTCTTCCTCCACTATAATCAATGAAGATGC
  AAGTTTCTTTCACCAGGGAGGGGTCCCTGGCGCTTCAGCTAATAATGGTGCTCTCTTGTTTCAAAATTTT
  CCCCATCATGTCAGCCCTGGCTTTGGTGGTAGCTTCTCCCCTCAGATCGGGCCTCTCTCCCAGCACCATC
  CTCATCACCCCCATTTCCAGCACCATCACAGCCAGCATCAGCAGCAGAGGAGGTCTCCTGCCAGTCCCCA
  CCCCCCACCTTTCACACATAGAAGTGCTGCTTTTAACCAGCTGCCTCATTTGGCGAATAATCTTAACAAA
  CCTCCTTCTCCATGGAGCAGCTACCAAAGTCCTTCTCCAACCCCCTCTTCTTCCTGGAGCCCAGGAGGTG
  GCGGCTACGGTGGCTGGGGAGCATCTCAAGGCCGGGATCACCGCAGAGGGCTGAACGGTGGAATAACACC
  CCTGAACTCAATCTCACCTTTGAAGAAAAATTTCGCAAGCAATCATATTCAGCTCCAGAAGTATGCTCGC
  CCTAGCTCAGCCTTTGCTCCAAAATCCTGGATGGAAGATAGCTTGAACAGGGCTGACAACATTTTTCCTT
  TTCCGGAACGCCCCAGGACGTTTGACATGCACTCACTGGAGAGCTCACTCATTGACATAATGAGAGCTGA
  AAATGATTCCATTAAAGGTCGTCTAAACTATTCATACCCAGGATCCGATAGTTCTCTGCTTATTAATGCA
  AGGACTTATGGGCGAAGGAGAGGTCAGTCTTCATTGTTTCCGATGGAAGATGGATTCCTGGATGATGGCC
  GTGGGGATCAACCTCTTCATAGTGGTCTGGGGTCACCTCACTGCTTCACTCACCAGAATGGGGAGAGAGT
  GGAACGATACTCTCGCAAAGTGTTTGTGGGTGGATTGCCTCCTGATATTGATGAAGATGAGATCACAGCT
  AGTTTCCGTCGCTTTGGCCCTTTGATTGTGGATTGGCCTCATAAGGCAGAGAGCAAATCTTATTTTCCAC
  CAAAAGGCTATGCATTCCTGCTGTTTCAAGATGAAAGTTCTGTTCAGGCTCTCATTGATGCATGTATTGA
  AGAAGATGGAAAACTTTACCTGTGTGTATCAAGTCCAACCATCAAGGATAAACCAGTGCAGATCCGGCCC
  TGGAATCTCAGTGACAGTGACTTTGTGATGGATGGCTCACAGCCACTTGACCCACGGAAAACAATATTTG
  TTGGTGGTGTTCCTCGACCATTACGAGCTGTGGAGCTTGCAATGATAATGGATCGGCTGTATGGAGGCGT
  CTGCTATGCTGGAATCGATACTGACCCAGAGCTCAAATACCCAAAAGGAGCTGGAAGAGTCGCGTTTTCT
  AATCAACAGAGTTACATAGCTGCTATCAGTGCCCGCTTTGTTCAGCTGCAGCATGGAGAGATAGATAAAC
  GGGTGGAGGTTAAGCCATATGTCTTGGATGACCAGCTGTGTGATGAATGTCAAGGGGCCCGTTGTGGGGG
  GAAATTTGCTCCATTTTTCTGTGCTAATGTTACCTGTCTGCAGTATTACTGTGAATATTGCTGGGCTGCT
  ATTCACTCTCGTGCTGGCAGAGAATTCCACAAGCCCCTGGTGAAGGAAGGTGGTGACCGCCCTCGGCATA
  TTTCATTCCGCTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224198 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDYGFGVLVQSNTGNKSAFPVRFHPHLQPPHHHQNATPNPAAFINNNTAANGSSAGSAWLFPAPATHNI
  QDEILGSEKAKSQQQEQQDPLEKQQLSPSPGQEAGILPETEKAKAEENPGDSSSENSNGKEKLRIESPVL
  TGFDYQEATGLGTSTQPLTSSASSLTGFSNWSAAIAPSSSTIINEDASFFHQGGVPGASANNGALLFQNF
  PHHVSPGFGGSFSPQIGPLSQHHPHHPHFQHHHSQHQQQRRSPASPHPPPFTHRSAAFNQLPHLANNLNK
  PPSPWSSYQSPSPTPSSSWSPGGGGYGGWGASQGRDHRRGLNGGITPLNSISPLKKNFASNHIQLQKYAR
  PSSAFAPKSWMEDSLNRADNIFPFPERPRTFDMHSLESSLIDIMRAENDSIKGRLNYSYPGSDSSLLINA
  RTYGRRRGQSSLFPMEDGFLDDGRGDQPLHSGLGSPHCFTHQNGERVERYSRKVFVGGLPPDIDEDEITA
  SFRRFGPLIVDWPHKAESKSYFPPKGYAFLLFQDESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRP
  WNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFS
  NQQSYIAAISARFVQLQHGEIDKRVEVKPYVLDDQLCDECQGARCGGKFAPFFCANVTCLQYYCEYCWAA
  IHSRAGREFHKPLVKEGGDRPRHISFRWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026252
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026252.2, NM_026252.3, NM_026252.4, NP_080528.2
• RefSeq Size: 7655 bp
• RefSeq ORF: 2190 bp
• Locus ID: 67579
• UniProt ID: Q7TN98
• Cytogenetics: 11 A4
• MW: 80.1 kDa
• Gene Summary: Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3' UTR (PubMed:17024188). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (By similarity). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (PubMed:28092655). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (By similarity). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (By similarity). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (PubMed:24386439).[UniProtKB/Swiss-Prot Function]