Adamts12 (NM_175501) Mouse Tagged ORF Clone

SKU MR224137

Adamts12 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 (Adamts12)

Product Data

• Target Symbol: Adamts12
• Synonyms: ADAMTS-12; AI605170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224137 representing NM_175501; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCATGTGCTCGGGGGAGCTGGCTGGCCAAGCTCTCGATCGTGGCTCAGCTTATTAATTTTGGGGCTT; TTTGCCACGGGAGACAAACTCAGCCCTGGCCGGTTCGCTTCCCAGACCCGAGGCAAGAACATTTTATCAA; GTCCCTGCCAGAATACCACATAGTGAGTCCAGTCCAAGTCGATGCTGGTGGCCATGTTCTGTCCTATGGC; CTGCATCACCCTGTCACTAGCAGCAGGAAGAAGAGAGCTGCAGGTGGTTCTGGGGACCAGCTGTACTACA; GAATTTCGCATGAAGAAAAAGACCTGTTTTTCAATCTGACGGTCAACTGGGAATTTCTTTCCAACGGCTA; TGTCGTGGAGAAGAGATATGGGAACCTTTCCCATGTTAAGATGGTGGCTTCCTCAGGCCAGCCCTGCCAT; CTCAGGGGCACAGTTCTGCAGCAGGGCACCACAGTCGGGATCGGGACAGCAGCTCTCAGTGCCTGCCAAG; GACTGACTGGATTTTTCCATCTACCACATGGAGACTTTTTCATCGAGCCTGTTAAAAAGCATCCACTGAC; CGAGGAAGGGTCCTACCCTCATGTCGTATACAGGAGACAGAGCATCAGAGCTCCAGAGACAAAGGAGCCC; ATCTGTGGATTAAAGGACAGTCTTGATAACTCTGTGAAACAAGAGCTACAGCGGGAGAAGTGGGAGAGGA; AAACCTTGCGGAGCAGAAGCCTCTCCCGACGTTCCATAAGCAAGGAGAGATGGGTGGAGACCCTCGTGGT; GGCCGACACGAAGACGGTAGAGTACCATGGAAGCGAGAACGTGGAGTCGTACATCCTCACCATCATGAAT; ATGGTCACTGGGTTGTTTCATAGCCCAAGCATTGGCAATCTAGTCCACATTGTTGTGGTTCGGCTCATTC; TACTTGAAGAAGAGGAGCAAGGATTGAAAATAGTTCATCATGCAGAGAAGACACTGTCCAGTTTCTGCAA; GTGGCAGAAGAGCATCAATCCCAAGAGTGACCTCAACCCTGTCCATCATGATGTGGCTGTCCTTATCACC; AGAAAGGACATCTGTGCTGGTGTCAATCGCCCTTGTGAGACTCTGGGGCTGTCCCAATTGTCAGGAATGT; GCCAGCCTCACAGGAGCTGTAACATCAATGAAGACTCTGGCCTCCCCCTGGCTTTCACCATTGCACATGA; ACTTGGTCACAGCTTCGGCATCCAGCATGATGGAAAGGAAAATGACTGTGAGCCCGTGGGCAGGCACCCA; TACATCATGTCCCAACAGATTCAGTACGACCCAACTCCACTAACATGGTCGAAATGCAGCAAGGAGTACA; TCACTCGCTTCTTGGACCGAGGCAGGGGGTTCTGTCTTGATGATATCCCCAGCAAGAAAGGCTTGAAGTC; CAACGTCATTGCTCCTGGAGTAATTTACGATGTTCATCACCAGTGCCAGCTCCAATATGGCCCAAATGCT; ACATTCTGCCAGGAAGTGGAAAATGTTTGCCAGACACTGTGGTGCTCGGTGAAAGGCTTCTGTCGCTCTA; AGCTAGATGCTGCTGCAGACGGGACTCGTTGCGGTGAGAAGAAGTGGTGTATGGCTGGAAAGTGTATCAC; AGTGGGGAAGAAACCAGAGAGCATCCCTGGAGGCTGGGGACGCTGGTCACCCTGGTCACACTGTTCTAGA; ACATGTGGGGCAGGAGCTCAGAGCGCAGAGAGGCTCTGCAACAACCCAGAACCAAAATTTGGAGGGAAAT; ACTGCACCGGAGAAAGAAAACGCTATCGCTTATGCAATGTCCACCCCTGCCGCTCAGACACTCCAACTTT; CCGGCAGATGCAGTGCAGTGAATTTGACACAGTCCCCTACAAAAACCAATTCTACCGCTGGTTCCCAGTG; TTTAATGCAGCACATCCTTGTGAGCTGTACTGCAGACCCATAGATGAACAGTTTTCTGAGAGAATGCTGG; AAGCTGTCATTGATGGCACCCCATGCTTCGAAGGTGGCAACAGCAGAAATGTCTGTATTAATGGCATATG; TAAGAGGGTTGGCTGTGACTATGAGATTGATTCCAATGCTACAGAGGATCGCTGTGGTGTGTGCCTTGGA; GATGGCTCTGCCTGCCAGACGGTGAAGAAGCTGTTTAGACAGAAGGAAGGATCTGGTTACGTTGACATTG; GACTTATTCCCAAAGGTGCGAGGGATATAAGGGTGATGGAAATCAAGGCAGCTGGCAATTTCCTGGCCAT; TAGGAGTGAAGATCCGGAAAAATACTACCTCAACGGAGGGTTCATTATCCAGTGGAATGGAAACTATAAA; CTGGCAGGGACCGTTTTCCAGTATGACAGGAAAGGAGATCTGGAGAAACTTATAGCTCCAGGCCCCACCA; ACGAGTCAGTGTGGCTCCAGCTACTTTTCCAGGTGACTAACCCTGGTATCAAGTATGAGTACACAGTCCG; GAAAGATGGCCTTGACAATGACGTGGAGAAGTTGCTGTACTTCTGGCAATTTGGCCGCTGGACAGAATGC; AGTGTAACGTGTGGAACAGGTATCCGCCGGCAGGCTGCTCATTGTGTCAAGAAGGGCCACGGTATAGTGA; AAACGACCTTCTGTAACCCAGAAACACAGCCCAGCGTGAGACAGAAGAAGTGCCATGAAAAGGACTGTCC; ACCCAGGTGGTGGGCAGGGGAGTGGGAAGCGTGTTCAACGACATGTGGGCCCTACGGAGAAAAAAAGAGA; ACGGTGCTGTGCATCCAGACCATGGGCTCGGATGAACAGGCTCTCCCTGCTACAGACTGCCAGCACCTGC; TGAAGCCCAAAGCACTGGTTTCCTGCAACAGAGATATTCTGTGTCCATCAGACTGGACAGTAGGCAACTG; GAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGCCAAGAACCTTAAT; GAGCCTTGTGACAAGACCCGGAAACCCAACAGTCGAGCTCTGTGCGGCCTCCAGCAGTGCCCATTTAGCC; GTAGAGTTCTGAAACCCAATAAAGATATAGCTCCCAGTGGGAAAAATCAATCGACAGCAGAACATGACCC; CTTCAAGCCCATCCCAGCACCAACATCCAGGCCCACGCCACTGTCCACACCCACAGTGCCTGAGTCTATG; AGCACAAGTACTCCCACAATCAACAGCCTTGGCTCCACCATAGCATCCCAAGAAGACGCAAATGGGATGG; GATGGCAGAATAATTCAACCCAAGCTGAAGAGGGCTCCCACTTCCCTACTTCTTCTGGAAGCACTTCCCA; GGTCCCCGTGACTTCCTGGTCTTTGAGTATCCAACCTGATGATGAGAATGTTTCCAGCTCAGCTATAGGT; CCTACTTCAGAGGGTGACTTTTGGGCAACCACAACAAGTGATTCTGGCTTATCATCTAGCGATGCTATGA; CTTGGCAGGTAACTCCATTTTATAGCACCATGACCACAGATCCAGAAGTGGAGATACATAGTGGTTCAGG; GGAAGACAGTGACCAGCCTTTGAACAAGGACAAAAGCAACTCTGTGATATGGAATAAGATCGGGGTACCT; GAACATGATGCTCCCATGGAGACGGATGCAGAACTACCACTTGGACCTCCACCAACATCTTATATGGGGG; AAGAGCCCTCATGGCCTCCCTTCAGTACAAAAATGGAAGGCTCATTACCTGCCTGGTCCTTCAAAAATGA; GACACCCAGGGATGATGGGATGATCGCTGAAAAATCAAGGAAGATTCCACTCCCTCTTGCAGGAGACCAC; CATCCAGCAACCTCAGAGAAGTTAGAAAACCATGACAAATTGGCATTGCCAAATACTACAAATCCAACCC; AAGGCTTTGGGCCTGTCCTGACTGAAGAAGATGCCTCCAATCTGATTGCCGAGGGGTTTTTGCTAAATGC; TTCTGATTACAAGCATCTTATGAAGGACCACAGCCCTGCATACTGGATTGTTGGAAACTGGAGTAAATGC; TCCACTACCTGTGGGCTGGGGGCCTACTGGAGGAGTGTGGAGTGCAGCTCTGGGGTGGACGCTGACTGCA; CAACCATTCAGAGGCCAGACCCTGCTAAGAAGTGCCACCTCCGCCCCTGTGCTGGCTGGCGAGTGGGAAA; CTGGAGCAAGTGCTCCCGAAATTGCAGTGGAGGCTTCAAGATTCGTGAGGTTCAGTGCATGGACAGCCTG; GACCACCACCGGAGCCTGAGGCCGTTTCACTGTCAATTCCTAGCTGGCGCTCCTCCTCCACTGAGTATGA; GCTGTAACCTGGAGCCTTGTGGAGAATGGCAAGTGGAGCCCTGGAGCCAGTGTTCTAGGTCCTGTGGAGG; AGGTGTTCAGGAGAGAGGAGTGTCTTGTCCTGGAGGCCTCTGTGACTGGACAAAAAGGCCTGCAACCACT; GTGCCCTGCAACAGACACCTTTGCTGTCACTGGGCCACTGGGAACTGGGAGCTGTGTAACACTTCCTGTG; GTGGTGGCTCTCAGAAAAGAACCATTCATTGCATCCCCTCAGAAAACAGTACAACTGAAGATCAAGACCA; GTGCCTCTGTGACCATCAAGTCAAACCCCCAGAATTCCAAACATGCAACCAGCAAGCCTGCAGGAAGAGT; GCTGATTTAACTTGCTTGAAGGACAGACTTTCAATCAGCTTCTGCCAGACACTGAAATCCATGAGGAAGT; GCTCTGTGCCATCAGTGCGAGCCCAGTGCTGTCTCTCGTGTCCACAGGCGCCCAGCATCCACACCCAGAG; GCAAAGAAAACAGCAGTTGCTCCAAAATCATGACATGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224137 representing NM_175501; Red=Cloning site Green=Tags(s); ; MPCARGSWLAKLSIVAQLINFGAFCHGRQTQPWPVRFPDPRQEHFIKSLPEYHIVSPVQVDAGGHVLSYG; LHHPVTSSRKKRAAGGSGDQLYYRISHEEKDLFFNLTVNWEFLSNGYVVEKRYGNLSHVKMVASSGQPCH; LRGTVLQQGTTVGIGTAALSACQGLTGFFHLPHGDFFIEPVKKHPLTEEGSYPHVVYRRQSIRAPETKEP; ICGLKDSLDNSVKQELQREKWERKTLRSRSLSRRSISKERWVETLVVADTKTVEYHGSENVESYILTIMN; MVTGLFHSPSIGNLVHIVVVRLILLEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDLNPVHHDVAVLIT; RKDICAGVNRPCETLGLSQLSGMCQPHRSCNINEDSGLPLAFTIAHELGHSFGIQHDGKENDCEPVGRHP; YIMSQQIQYDPTPLTWSKCSKEYITRFLDRGRGFCLDDIPSKKGLKSNVIAPGVIYDVHHQCQLQYGPNA; TFCQEVENVCQTLWCSVKGFCRSKLDAAADGTRCGEKKWCMAGKCITVGKKPESIPGGWGRWSPWSHCSR; TCGAGAQSAERLCNNPEPKFGGKYCTGERKRYRLCNVHPCRSDTPTFRQMQCSEFDTVPYKNQFYRWFPV; FNAAHPCELYCRPIDEQFSERMLEAVIDGTPCFEGGNSRNVCINGICKRVGCDYEIDSNATEDRCGVCLG; DGSACQTVKKLFRQKEGSGYVDIGLIPKGARDIRVMEIKAAGNFLAIRSEDPEKYYLNGGFIIQWNGNYK; LAGTVFQYDRKGDLEKLIAPGPTNESVWLQLLFQVTNPGIKYEYTVRKDGLDNDVEKLLYFWQFGRWTEC; SVTCGTGIRRQAAHCVKKGHGIVKTTFCNPETQPSVRQKKCHEKDCPPRWWAGEWEACSTTCGPYGEKKR; TVLCIQTMGSDEQALPATDCQHLLKPKALVSCNRDILCPSDWTVGNWSECSVSCGGGVRIRSVTCAKNLN; EPCDKTRKPNSRALCGLQQCPFSRRVLKPNKDIAPSGKNQSTAEHDPFKPIPAPTSRPTPLSTPTVPESM; STSTPTINSLGSTIASQEDANGMGWQNNSTQAEEGSHFPTSSGSTSQVPVTSWSLSIQPDDENVSSSAIG; PTSEGDFWATTTSDSGLSSSDAMTWQVTPFYSTMTTDPEVEIHSGSGEDSDQPLNKDKSNSVIWNKIGVP; EHDAPMETDAELPLGPPPTSYMGEEPSWPPFSTKMEGSLPAWSFKNETPRDDGMIAEKSRKIPLPLAGDH; HPATSEKLENHDKLALPNTTNPTQGFGPVLTEEDASNLIAEGFLLNASDYKHLMKDHSPAYWIVGNWSKC; STTCGLGAYWRSVECSSGVDADCTTIQRPDPAKKCHLRPCAGWRVGNWSKCSRNCSGGFKIREVQCMDSL; DHHRSLRPFHCQFLAGAPPPLSMSCNLEPCGEWQVEPWSQCSRSCGGGVQERGVSCPGGLCDWTKRPATT; VPCNRHLCCHWATGNWELCNTSCGGGSQKRTIHCIPSENSTTEDQDQCLCDHQVKPPEFQTCNQQACRKS; ADLTCLKDRLSISFCQTLKSMRKCSVPSVRAQCCLSCPQAPSIHTQRQRKQQLLQNHDML; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175501
• ORF Size: 4800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_175501.3, NP_780710.2
• RefSeq Size: 8622 bp
• RefSeq ORF: 4803 bp
• Locus ID: 239337
• UniProt ID: Q811B3
• Cytogenetics: 15 A1
• MW: 177.8 kDa
• Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active protease. Mice lacking the encoded protein exhibit increased angiogenic response and tumor invasion in different models of angiogenesis and, severe inflammation and delayed recovery when subjected to experimental conditions that induce colitis, endotoxic sepsis and pancreatitis. [provided by RefSeq, Jul 2016]