Fndc4 (NM_022424) Mouse Tagged ORF Clone

CAT#: MR223815

Fndc4 (Myc-DDK-tagged) - Mouse fibronectin type III domain containing 4 (Fndc4)

Product Images

Circular map for MR223815

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fndc4
• Synonyms: 2810430J06Rik; 6330410H20Rik; AB030187; AI838506; AW487863; Fnmp1; FRCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223815 representing NM_022424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTGGCCCCTCCAGCGAACTCGGTGGAAACCATGGCTTCTTTGATGCCTCTTTCCCCATATCTGA
  GTCCCACGGTCCTCCTGCTGGTCAGCTGTGACCTGGGTTTTGTGCGAGCAGACCGACCTCCCTCTCCTGT
  GAATGTGACGGTCACTCATCTCAGAGCCAACTCAGCCACTGTGTCCTGGGACGTTCCAGAAGGCAACATC
  GTCATTGGCTACTCCATTTCCCAGCAACGACAGAATGGCCCGGGGCAGCGTGTAATCAGAGAAGTGAACA
  CCACCACCAGGGCCTGCGCTCTCTGGGGCCTGGCTGAAGACAGTGACTACACAGTGCAGGTTAGGAGCAT
  CGGCCTCCGGGGAGAGAGCCCTCCAGGGCCCCGTGTGCACTTCCGCACGCTCAAGGGTTCTGACAGGCTG
  CCCTCCAACAGCTCCAGTCCAGGTGATATTACAGTGGAGGGTCTGGATGGAGAGCGGCCATTGCAGACAG
  GGGAAGTCGTCATCATTGTAGTGGTATTGCTCATGTGGGCTGCTGTAATCGGGCTATTCTGCCGTCAGTA
  TGACATCATCAAGGACAACGACTCCAACAACAACCCCAAGGAGAAGGGGAAGGGGCCTGAACAGAGTCCT
  CAGGGAAGGCCAGTGGGGACAACAAGACAGAAAAAGTCTCCATCAATCAACACCATTGATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223815 representing NM_022424
  Red=Cloning site Green=Tags(s)
  
  MPLAPPANSVETMASLMPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVPEGNI
  VIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRL
  PSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGPEQSP
  QGRPVGTTRQKKSPSINTIDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022424
• ORF Size: 693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022424.6
• RefSeq Size: 2270 bp
• RefSeq ORF: 696 bp
• Locus ID: 64339
• UniProt ID: Q3TR08
• Cytogenetics: 5 B1
• MW: 25.4 kDa
• Gene Summary: Acts as an anti-inflammatory factor in the intestine and colon. Binds to and acts on macrophages to downregulate pro-inflammatory gene expression. Affects key macrophage functions, including phagocytosis, by downregulating many key pathways for macrophage activation, partly via by STAT3 activation and signaling. May be required to dampen the immunological response in colitis.[UniProtKB/Swiss-Prot Function]