Atp1b3 (NM_007502) Mouse Tagged ORF Clone

CAT#: MR223683

Atp1b3 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, beta 3 polypeptide (Atp1b3)

Product Images

Circular map for MR223683

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp1b3
• Synonyms: AA409958; AI664000; AW212096
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223683 representing NM_007502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAAGACTGAGAAGAAATCCTTCCACCAGAGCCTGGCCGAGTGGAAGCTGTTCATCTACAACCCGA
  GCAGCGGAGAGTTTCTGGGGCGCACCTCCAAGAGCTGGGGTCTGATCTTGCTCTTCTACCTAGTTTTTTA
  TGGGTTCTTGGCTGCACTCTTCACATTCACAATGTGGGCCATGCTCCAGACTCTGAATGATGAAGTTCCG
  AAATATCGAGACCAGATTCCTAGTCCAGGACTTATGGTTTTTCCCAAACCGCAGACTGCCTTGGAATATA
  CATTCAGCATGTCTGAGCCACAGACTTACAAAAAGTTGGTTGAAGACCTGGAGAGTTTCCTAAAGCCATA
  TTCTGTGGAAGAACAGAAGAACCTCACAAGTTGTCCTGATGGAGCGCCTTTTATTCAGCATGGTCCTGAC
  TATAGGGCATGTCAGTTTCCAGTCTCCTTGCTTGAAGAATGTAGTGGTGTGACTGATGCTAATTTTGGCT
  ATTCCAAAGGACAGCCTTGCATCCTTGTGAAAATGAACAGAATAATCGATTTAATCCCAGACGGATATCC
  ACAAATATCGTGTTTGCCAAAGGAAGAAAACGCAACTATAGCAACTTATCCTGAATTTGGAGTTTTAGAC
  TTAAAGTATTTTCCATATTATGGGAAAAAACGGCATGTTGGATATCGACAGCCCCTAGTTGCCGTACAGG
  TCAAATTTGACTCTGGTCTTAACAAGAAAGAAGTAACAGTTGAGTGCCATATTGCTGGAACCAGGAACCT
  AAAAAACAAGAATGAGCGTGACAAGTTCTTGGGACGTGTTTCGTTCAAAGTCACAGCACGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223683 representing NM_007502
  Red=Cloning site Green=Tags(s)
  
  MTKTEKKSFHQSLAEWKLFIYNPSSGEFLGRTSKSWGLILLFYLVFYGFLAALFTFTMWAMLQTLNDEVP
  KYRDQIPSPGLMVFPKPQTALEYTFSMSEPQTYKKLVEDLESFLKPYSVEEQKNLTSCPDGAPFIQHGPD
  YRACQFPVSLLEECSGVTDANFGYSKGQPCILVKMNRIIDLIPDGYPQISCLPKEENATIATYPEFGVLD
  LKYFPYYGKKRHVGYRQPLVAVQVKFDSGLNKKEVTVECHIAGTRNLKNKNERDKFLGRVSFKVTARA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007502
• ORF Size: 834 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007502.5
• RefSeq Size: 1833 bp
• RefSeq ORF: 837 bp
• Locus ID: 11933
• UniProt ID: P97370
• Cytogenetics: 9 50.31 cM
• MW: 32.2 kDa
• Gene Summary: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.[UniProtKB/Swiss-Prot Function]