Asph (NM_023066) Mouse Tagged ORF Clone

CAT#: MR223585

Asph (Myc-DDK-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 1

Product Images

Circular map for MR223585

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asph
• Synonyms: 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223585 representing NM_023066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGCGCAAGAACGCCAAGGGCGGCGGCGGCAACAGCAGCAGCAGCGGCAGCGGCAGCGGCTCGG
  GCAGCGGTAGCCCGAGCACGGGTAGCAGCGGTAGCAGCAGTAGCCCCGGGGCTCGGAGAGAGGCAAAGCA
  CGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGTCCTTTTTCACATGGTTCATGGTCATT
  GCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGACTTGGTCGATTATGAAGAAGTTCTAG
  GAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGATGTGGATGATGCCAAAGTTTTATTAGG
  CCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTG
  GAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGATGAAGTAAAGGAACAAATTCAGTCCC
  TTCTTCAGGAATCAGTACACACAGACCATGACCTTGAAGCAGATGGGCTGGCAGGAGAACCACAGCCGGA
  GGTTGAGGACTTCCTCACAGTGACCGACAGTGATGACAGATTTGAGGACCTGGAACCCGGGACAGTTCAT
  GAAGAAATCGAGGATACTTACCATGTGGAAGACACAGCATCGCAGAACCATCCAAATGACATGGAAGAGA
  TGACGAATGAGCAGGAAAATTCAGATCCCAGTGAAGCAGTGACAGATGCAGGTGTGCTGCTGCCCCATGC
  AGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAACCCTCAGAGCATGAAGGGGTCGCGATT
  TCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAATAAATGTCGCCTCTGTTGAAGAACAGC
  AAGACACACCACCAGTTAAGAAGAAGAAGCCTAAACTTCTGAACAAATTTGATAAGACAATTAAGGCTGA
  GCTGGATGCTGCAGAAAAGCTCCGGAAAAGGGGTAAAATTGAGGAAGCAGTGAACGCATTTGAAGAACTG
  GTTCGAAAGTACCCTCAGAGCCCACGAGCAAGATATGGCAAAGCGCAGTGTGAAGATGACTTGGCAGAGA
  AGCAGAGAAGCAATGAGGTTCTGCGCAGGGCCATCGAGACCTACCAGGAGGCAGCCGACCTGCCTGATGC
  CCCTACAGACCTGGTGAAGCTGAGCTTGAAGCGAAGGTCGGAACGGCAGCAGTTTCTAGGTCACATGAGA
  GGTTCTCTACTTACCCTCCAGAGACTAGTTCAACTGTTCCCTAGTGATACTACCTTAAAAAACGACCTTG
  GCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTTACGAAGAGGTGCTAAATGTGAC
  ACCAAATGATGGCTTCGCTAAAGTGCATTACGGCTTCATCCTGAAGGCACAGAACAAGATATCTGAGAGC
  ATTCCCTACTTAAAGGAAGGAATCGAATCTGGGGACCCTGGCACGGATGATGGCCGGTTTTACTTCCACT
  TGGGGGATGCCATGCAGAGGGTCGGGAACAAAGAGGCATATAAGTGGTATGAACTTGGGCACAAGAGAGG
  ACATTTTGCCTCTGTCTGGCAGCGTTCCCTCTACAATGTGAATGGTCTGAAGGCTCAGCCGTGGTGGACA
  CCCAGGGAGACTGGCTACACAGAGCTAGTGAAGTCTTTAGAGAGAAACTGGAAGTTAATCCGTGATGAAG
  GCCTCATGGTGATGGATAAAGCCAAGGGTCTCTTCCTGCCTGAGGACGAAAACCTTCGGGAGAAGGGCGA
  CTGGAGCCAGTTCACACTGTGGCAGCAAGGAAGGAAGAATGAGAATGCCTGTAAAGGAGCGCCTAAGACC
  TGTGCTTTACTAGAAAAGTTTTCCGAAACAACAGGATGCAGAAGAGGACAGATCAAATACTCCATCATGC
  ACCCTGGAACTCATGTGTGGCCGCATACAGGACCCACAAACTGCAGGCTCCGAATGCATCTGGGGTTAGT
  GATCCCCAAGGAAGGCTGCAAGATCCGGTGTGCCAATGAGACCAGGACGTGGGAAGAAGGCAAGGTGCTC
  ATCTTTGATGACTCTTTTGAGCACGAGGTTTGGCAGGATGCCTCGTCTTTCCGGCTGATATTCATCGTGG
  ATGTGTGGCACCCCGAGCTGACCCCTCAGCAGAGACGCAGCCTTCCCGCAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223585 representing NM_023066
  Red=Cloning site Green=Tags(s)
  
  MAPRKNAKGGGGNSSSSGSGSGSGSGSPSTGSSGSSSSPGARREAKHGGHKNGRRGGISGGSFFTWFMVI
  ALLGVWTSVAVVWFDLVDYEEVLGKLGVYDADGDGDFDVDDAKVLLGLKERSPSERTFPPEEEAETHAEL
  EEQAPEGADIQNVEDEVKEQIQSLLQESVHTDHDLEADGLAGEPQPEVEDFLTVTDSDDRFEDLEPGTVH
  EEIEDTYHVEDTASQNHPNDMEEMTNEQENSDPSEAVTDAGVLLPHAEEVRHQDYDEPVYEPSEHEGVAI
  SDNTIDDSSIISEEINVASVEEQQDTPPVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNAFEEL
  VRKYPQSPRARYGKAQCEDDLAEKQRSNEVLRRAIETYQEAADLPDAPTDLVKLSLKRRSERQQFLGHMR
  GSLLTLQRLVQLFPSDTTLKNDLGVGYLLLGDNDSAKKVYEEVLNVTPNDGFAKVHYGFILKAQNKISES
  IPYLKEGIESGDPGTDDGRFYFHLGDAMQRVGNKEAYKWYELGHKRGHFASVWQRSLYNVNGLKAQPWWT
  PRETGYTELVKSLERNWKLIRDEGLMVMDKAKGLFLPEDENLREKGDWSQFTLWQQGRKNENACKGAPKT
  CALLEKFSETTGCRRGQIKYSIMHPGTHVWPHTGPTNCRLRMHLGLVIPKEGCKIRCANETRTWEEGKVL
  IFDDSFEHEVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023066
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023066.3, NP_075553.2
• RefSeq Size: 6688 bp
• RefSeq ORF: 2226 bp
• Locus ID: 65973
• UniProt ID: Q8BSY0
• Cytogenetics: 4 A1
• MW: 83.5 kDa
• Gene Summary: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]