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Rac3 (NM_133223) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223529
Rac3 (Myc-DDK-tagged) - Mouse RAS-related C3 botulinum substrate 3 (Rac3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Rac3 or NM_133223"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rac3
Synonyms Rac1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223529 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCCATCAAGTGCGTGGTGGTTGGCGATGGTGCCGTGGGGAAGACGTGCCTGCTGATCAGCTACA
CGACCAACGCCTTCCCAGGAGAATATATCCCCACAGTTTTCGACAACTACTCAGCCAACGTGATGGTGGA
TGGGAAGCCAGTTAACCTGGGGCTGTGGGACACTGCTGGCCAGGAAGACTACGATAGGCTTCGGCCACTC
TCCTATCCTCAAACAGATGTCTTTCTGATCTGCTTCTCCCTGGTGAGCCCAGCCTCCTTTGAGAATGTCC
GTGCCAAGTGGTACCCAGAGGTGCGGCACCACTGCCCACACACACCCATCCTTCTGGTGGGCACCAAGCT
GGACCTCCGTGATGACAAGGATACGATTGAACGGCTGCGGGACAAGAAGCTGGCACCCATAACCTACCCC
CAAGGCCTGGCCATGGCCCGAGAGATTGGTTCCGTCAAGTACCTGGAGTGCTCAGCTCTGACCCAGAGAG
GTCTGAAGACAGTGTTCGACGAGGCCATCCGGGCTGTGCTCTGCCCACCTCCAGTGAAAAAGCCAGGCAA
GAAGTGCACTGTATTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223529 protein sequence
Red=Cloning site Green=Tags(s)

MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPL
SYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPHTPILLVGTKLDLRDDKDTIERLRDKKLAPITYP
QGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKPGKKCTVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133223
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133223.4, NP_573486.1
RefSeq Size 1078 bp
RefSeq ORF 579 bp
Locus ID 170758
UniProt ID P60764
Cytogenetics 11 E2
MW 21.4 kDa
Summary Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner (By similarity).UniProtKB/Swiss-Prot Function
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"NM_133223" in other vectors (4)
SKU Description Size Price
MC212334 Rac3 (untagged) - Mouse RAS-related C3 botulinum substrate 3 (Rac3), (10ug) 10 ug
$330.00
MG223529 Rac3 (tGFP-tagged) - Mouse RAS-related C3 botulinum substrate 3 (Rac3), (10ug) 10 ug
$489.00 $500.00
MR223529L3 Lenti ORF clone of Rac3 (Myc-DDK-tagged) - Mouse RAS-related C3 botulinum substrate 3 (Rac3) 10 ug
$600.00
MR223529L4 Lenti ORF clone of Rac3 (mGFP-tagged) - Mouse RAS-related C3 botulinum substrate 3 (Rac3) 10 ug
$600.00

Plasmid Map for MR223529

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223529
Circular map for MR223529

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