Col6a1 (NM_009933) Mouse Tagged ORF Clone

CAT#: MR223027

Col6a1 (Myc-DDK-tagged) - Mouse collagen, type VI, alpha 1 (Col6a1)

Product Images

Circular map for MR223027

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col6a1
• Synonyms: AI747156; Col6a-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223027 representing NM_009933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGGCCCACGCTCTGCTGCCCCTGCTGCTACAAGCCTGCTGGGTGGCCACACAGGACATCCAGG
  GCTCCAAAGCGATTGCCTTCCAAGACTGCCCTGTGGATCTATTCTTCGTGCTCGACACCTCGGAGAGTGT
  GGCCTTGAGGCTGAAACCTTATGGGGCCTTGGTGGACAAGGTGAAGTCCTTCACTAAGCGCTTCATTGAC
  AACCTGAGAGACAGGTACTACCGGTGTGACCGCAACCTGGTTTGGAATGCGGGTGCGCTGCACTACAGTG
  ACGAGGTGGAGATCATCCGAGGGCTCACGCGCATGCCCAGTGGCCGCGATGAGCTCAAGGCCAGCGTGGA
  TGCGGTCAAGTACTTCGGGAAAGGCACCTACACCGACTGCGCCATTAAGAAGGGGCTGGAGGAGCTGCTC
  ATAGGGGGCTCCCACCTGAAGGAGAACAAGTACTTGATCGTGGTGACCGACGGGCATCCTCTAGAGGGCT
  ACAAGGAACCATGCGGGGGTCTGGAAGATGCAGTAAATGAGGCCAAACACCTGGGCATCAAGGTCTTTTC
  TGTGGCCATCACACCTGACCACCTGGAGCCACGTCTAAGTATCATTGCCACAGACCACACATACCGGCGC
  AATTTCACGGCAGCTGACTGGGGGCATAGCCGCGATGCAGAAGAGGTCATCAGCCAGACCATTGACACCA
  TTGTGGACATGATTAAAAATAACGTGGAACAAGTGTGTTGTTCTTTTGAGTGCCAGGCTGCCAGAGGACC
  TCCAGGGCCCCGAGGCGACCCTGGGTATGAGGGGGAGCGAGGAAAGCCAGGTCTTCCGGGAGAGAAGGGA
  GAAGCTGGAGACCCTGGACGACCTGGGGATCTTGGACCAGTCGGGTACCAGGGTATGAAGGGAGAAAAGG
  GGAGCCGTGGAGAGAAGGGTTCCAGAGGACCGAAAGGTTACAAGGGCGAGAAAGGCAAGCGCGGAATCGA
  CGGGGTCGACGGCATGAAGGGAGAGACGGGGTACCCAGGACTACCGGGCTGCAAGGGCTCCCCAGGATTT
  GATGGCATTCAAGGACCCCCGGGTCCCAAGGGTGATGCTGGTGCCTTTGGGATGAAGGGAGAAAAGGGTG
  AAGCTGGAGCAGACGGTGAGGCTGGGAGACCAGGGAACTCAGGGTCACCTGGAGATGAGGGTGATCCTGG
  AGAGCCTGGTCCCCCCGGAGAAAAAGGAGAGGCCGGTGATGAAGGAAATGCTGGCCCAGACGGTGCCCCT
  GGAGAGAGGGGTGGCCCTGGTGAAAGAGGACCTCGGGGGACCCCTGGTGTGAGAGGACCAAGGGGAGACC
  CGGGTGAAGCTGGACCACAGGGTGACCAAGGAAGAGAGGGGCCCGTCGGCATCCCTGGAGACTCGGGTGA
  GGCTGGCCCCATTGGACCTAAAGGATACCGAGGTGATGAGGGTCCTCCAGGTCCTGAGGGCCTCAGAGGA
  GCCCCAGGACCTGTTGGTCCTCCTGGAGACCCCGGACTGATGGGTGAGAGAGGTGAGGATGGACCACCAG
  GAAACGGCACGGAAGGTTTCCCCGGCTTCCCTGGGTATCCAGGCAACAGAGGCCCTCCTGGGCTAAATGG
  CACAAAAGGCTACCCTGGCCTCAAGGGGGATGAGGGTGAAGTGGGAGACCCAGGAGAGGATAACAACGAC
  ATTTCACCCCGTGGGGTCAAAGGGGCAAAGGGATACCGAGGCCCAGAAGGACCCCAGGGACCTCCAGGAC
  ATGTGGGACCACCTGGGCCAGATGAGTGTGAGATCCTGGATATCATCATGAAAATGTGCTCCTGCTGTGA
  GTGCACATGTGGACCCATTGACATCCTCTTCGTGCTGGACAGCTCGGAGAGCATTGGCCTACAGAACTTT
  GAGATTGCCAAGGACTTCATCATCAAGGTCATTGACCGGTTGAGCAAGGATGAGCTGGTCAAATTTGAGC
  CAGGGCAGTCTCACGCGGGCGTGGTACAGTACAGCCACAACCAGATGCAAGAGCACGTGGACATGCGGAG
  CCCCAACGTCCGCAACGCCCAGGACTTCAAAGAAGCTGTCAAGAAGCTACAATGGATGGCTGGTGGCACA
  TTCACCGGAGAAGCGCTGCAGTACACCCGGGACCGGCTACTCCCACCCACACAGAACAACCGAATTGCCC
  TGGTCATTACGGATGGACGTTCTGACACTCAACGGGACACGACACCTCTCAGTGTGCTCTGTGGTGCAGA
  CATTCAGGTAGTTTCTGTGGGAATCAAGGATGTGTTTGGCTTTGTGGCGGGCTCCGACCAGCTCAATGTC
  ATTTCCTGCCAAGGCTTATCGCAAGGTCGGCCAGGTATCTCCCTGGTGAAGGAGAACTATGCAGAGCTTC
  TCGATGACGGCTTTCTGAAGAACATAACAGCCCAGATCTGTATAGATAAGAAGTGTCCGGATTATACCTG
  TCCAATCACATTCTCCTCCCCGGCTGACATCACCATCCTGCTAGACAGCTCAGCCAGTGTCGGCAGCCAC
  AACTTCGAAACCACCAAGGTCTTCGCCAAGCGCCTAGCTGAGCGATTCCTGTCAGCAGGCAGGGCGGATC
  CTTCCCAGGATGTGCGGGTGGCCGTGGTACAGTATAGTGGCCAGGGGCAGCAACAGCCAGGTCGGGCGGC
  TCTTCAGTTCTTACAGAATTACACAGTGCTGGCCAGCTCTGTGGACAGCATGGATTTCATCAACGACGCC
  ACAGACGTCAACGATGCTCTGAGCTACGTGACTCGTTTCTACCGGGAAGCCTCGTCAGGTGCCACCAAGA
  AGAGAGTGCTGTTGTTTTCAGACGGCAACTCTCAGGGGGCCACAGCAGAGGCCATTGAGAAGGCTGTGCA
  GGAGGCCCAGCGTGCAGGCATTGAGATCTTTGTGGTGGTGGTGGGACCCCAGGTGAACGAGCCCCACATC
  CGTGTGCTTGTCACTGGCAAGACTGCAGAGTACGACGTGGCCTTTGGCGAGCGCCACCTATTCCGTGTAC
  CAAACTACCAGGCCCTGCTACGTGGCGTACTCTACCAGACAGTCTCCAGGAAGGTGGCACTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223027 representing NM_009933
  Red=Cloning site Green=Tags(s)
  
  MRLAHALLPLLLQACWVATQDIQGSKAIAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFID
  NLRDRYYRCDRNLVWNAGALHYSDEVEIIRGLTRMPSGRDELKASVDAVKYFGKGTYTDCAIKKGLEELL
  IGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGIKVFSVAITPDHLEPRLSIIATDHTYRR
  NFTAADWGHSRDAEEVISQTIDTIVDMIKNNVEQVCCSFECQAARGPPGPRGDPGYEGERGKPGLPGEKG
  EAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGIDGVDGMKGETGYPGLPGCKGSPGF
  DGIQGPPGPKGDAGAFGMKGEKGEAGADGEAGRPGNSGSPGDEGDPGEPGPPGEKGEAGDEGNAGPDGAP
  GERGGPGERGPRGTPGVRGPRGDPGEAGPQGDQGREGPVGIPGDSGEAGPIGPKGYRGDEGPPGPEGLRG
  APGPVGPPGDPGLMGERGEDGPPGNGTEGFPGFPGYPGNRGPPGLNGTKGYPGLKGDEGEVGDPGEDNND
  ISPRGVKGAKGYRGPEGPQGPPGHVGPPGPDECEILDIIMKMCSCCECTCGPIDILFVLDSSESIGLQNF
  EIAKDFIIKVIDRLSKDELVKFEPGQSHAGVVQYSHNQMQEHVDMRSPNVRNAQDFKEAVKKLQWMAGGT
  FTGEALQYTRDRLLPPTQNNRIALVITDGRSDTQRDTTPLSVLCGADIQVVSVGIKDVFGFVAGSDQLNV
  ISCQGLSQGRPGISLVKENYAELLDDGFLKNITAQICIDKKCPDYTCPITFSSPADITILLDSSASVGSH
  NFETTKVFAKRLAERFLSAGRADPSQDVRVAVVQYSGQGQQQPGRAALQFLQNYTVLASSVDSMDFINDA
  TDVNDALSYVTRFYREASSGATKKRVLLFSDGNSQGATAEAIEKAVQEAQRAGIEIFVVVVGPQVNEPHI
  RVLVTGKTAEYDVAFGERHLFRVPNYQALLRGVLYQTVSRKVALG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009933
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009933.4, NP_034063.1
• RefSeq Size: 3990 bp
• RefSeq ORF: 3078 bp
• Locus ID: 12833
• UniProt ID: Q04857
• Cytogenetics: 10 39.71 cM
• MW: 108.5 kDa
• Gene Summary: Collagen VI acts as a cell-binding protein.[UniProtKB/Swiss-Prot Function]