Adam22 (NM_001007220) Mouse Tagged ORF Clone

CAT#: MR222778

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Adam22 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 1



  "NM_001007220" in other vectors (4)

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USD 900.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Adam22 (NM_001007220) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Adam22
Synonyms 2900022I03Rik; AI854032; MDC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR222778 representing NM_001007220
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCAGCGGCGGCCGCGTCCTTCTGGCTGCTCTGCGTCTTGGGGACCTGCCCCCTGGCGCGCTGCG
GTCGGGCAGGAGTCGCCTCACTGAAAGGACTGGAGAGGGGGAAAGAAAACCGCTTCCTGGAGCGCCAGAG
CATCATACCACTGCGCCTCATCTATCGCTTGGGCGGCGAGGATGAAACTCAGCACAACCAGCTCGACACG
CGGGTGCGGGGCGACCCTGGCGGACCGCAGCTGACGCATGTGGACAAGGCAAGCTTCCGGGTGGACGCCT
TTGGAACCTCTTTCGTTCTCGACGTCCTGCTGAACCACGAGTTGCTGTCGTCAGGTTATGTAGAGAGACA
GATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGA
GGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACC
ACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAA
ATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGAATTTCAACGCGTAAACATTACTCCCCCT
CAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGG
AGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGT
TGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAA
ACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCAC
TGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCT
TTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTG
AGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCC
ACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTC
CGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATAT
CATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTG
AGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGA
AGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAA
AAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATAT
GTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCA
GGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTA
ACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAG
ACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGG
CAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACA
TTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGC
CCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTG
CTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGC
AACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCA
CCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCT
GGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGACAGTTACCCCAGGGA
GATTATGTAAAAAAGCCTGGCGATGGCGACTCTTTTTATAGCGACTTTCCTCCCGGAGGCAGCACAAACT
CAGCATCTAGTTCTAAGAAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAA
ACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAAC
AAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGAG


CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
ATAA
GGTTTAA
>MR222778 representing NM_001007220
Red=Cloning site Green=Tags(s)

MQAAAAASFWLLCVLGTCPLARCGRAGVASLKGLERGKENRFLERQSIIPLRLIYRLGGEDETQHNQLDT
RVRGDPGGPQLTHVDKASFRVDAFGTSFVLDVLLNHELLSSGYVERQIEHGGKVVENKGGEHCYYQGQIR
GNPVSFVALSTCHGLHGMFYDGNHTYLIEPEENEKSQESSHCHSVYKSRQFEFPLDDLPSEFQRVNITPP
QFILKPRLKRRKRQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLK
TRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLL
RGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEY
HDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCK
KCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKV
TASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRT
LNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVC
NRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRQLPQG
DYVKKPGDGDSFYSDFPPGGSTNSASSSKKRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNMGGN
KKKIRGKRFRPRSNSTE

LEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-XhoI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001007220
ORF Size 2571 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001007220.1, NM_001007220.2, NM_001007220.3, NP_001007221.1
RefSeq Size 2777 bp
RefSeq ORF 2574 bp
Locus ID 11496
Cytogenetics 5 3.39 cM
MW 95.2 kDa
Gene Summary This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit severe ataxia, hypomyelination and premature death. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]

Citations (1)

Other Versions

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