Amer2 (NM_028113) Mouse Tagged ORF Clone

CAT#: MR222762

Amer2 (Myc-DDK-tagged) - Mouse family with sequence similarity 123, member A (Fam123a), transcript variant 1

Product Images

Circular map for MR222762

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Amer2
• Synonyms: 2600011E07Rik; Fam123a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222762 representing NM_028113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGCATTGTGAGTGCGCCGCGGAGACGCCCGCCGCAGAGCCGCCGTCGGGGAAGATCAATAAGG
  CTGCGTTCAAGTTATTCAAGAAAAGGAAATCGGGGGGCACCATGCCCAGCATTTTTGGGGTCAAAAACAA
  AGGGGATGGGAAGAGCTCGGGGCCGACGGGAATGGTGAGAAGCAGGACCCACGACGGACTAGCCGAGGTG
  TTGGTGCTGGAGGGCAGCAAGAAGGAGGAGCCGCCTGGCGGGAGCGATCACAGCGGGGCCCGGCCGATCC
  CCGGACCCCCCAAACCCTCCGGGCCTGGCCTGGGCTCCCTGGCCAGCAGCTCGGTGGCCAAGTCCCACAG
  CTTCTTCTCCCTGCTGAAAAAGAACGGGCGATCGGAGACCGGCAAGGGAGACCATGCCGAGGCGAGCAAG
  GCTGGCGGCAAACAAAAGAGGGGGCTGAAAGGGATCTTCAGCAGCATGCGCTGGCACCGGAGGGACAAGC
  GCGGCAAGGAGGAGGAGGAGAAGGCGGTGCGCGCGGCGGGCCCGGGCAACCTGGTTCTGCCCGGTTCGCT
  CACCGCCAGCCTGGAGTGCGTCAAGGAGGAGCCGCCCCGAGCCGCGCGCCGCCCGGACAGCCCGGGCCAG
  GACGCCTCGCGACACGCAGCAGGTGAGCCCGCAGGGGGAGAGCAGGCGCCCGCGTCCGCCGAGAGCGCCC
  CGGAGCGGATCTGCCTCGAGGCCGGGAGCCCCACCGGCTCTGGCGATCAAAGCTCCCGGGGAGAGGACGC
  CGAGGGGCATCGGCGCGAGGAGAAGCCCGGGGCAGCCCTCGAGTCGGGAGCCGGTGAGGTCCAGGCGGCC
  GAGGATGCGTCCAAGACAGGTGACGTTCCGATAAAGACCGTCCCCCTTGTCGACTCCGAAGGTGGCAGCG
  GCCGGGCGTCTGCTGTCCCTGACCCTTCCTCTGTTGATCCACCCTCAGACCCGTCGGCAGATCGTATTTG
  TTTGATGTTTTCTGACGTGACTTCACTGAAAAGCTTTGACTCTCTTACAGGCTGTGGAGATATTATTGCA
  GACCCAGAAGAAGAGGCAGGCCCCAGCTGTGACAAGCATGTCCCCGGGCCAGGCAAGCCAGTTCTCTCTA
  AAAAGAACGCCAGCGTGGTGGCCTACCAAGGAGGAGGGGAGGAGATGGCCAGCCCGGATCAGGTGGACGA
  CACCTATCTCCCGGAATTCTGGGACATGTTGTCCCAGACTGAGGACCAAGGACAAGGAACTCAAGAAGGC
  GCAGCGAAGGCAGCCACTGCTTCCGACATCAAGTTGGCCCCTGAGACCTCCAGTGATACCCGGTGTGGGG
  AAGCAGCCAAGGACATGTCCTCGGTCAAGCGCAGGAGGCTCCACAGAATTCCCATTGAGTCCCAGCAGAA
  GGAGGAGCCCAAACATCCGGAGAAGGAGCATCAAGAAGGTGTTCCTAACAGCGATGAGGGCTACTGGGAC
  TCCACCACCCCTGGCCCAGAAGAAGAGAGCATCAGCAACAGCAGCAGCAGCAAGAAGGTGGTCATCCCCA
  GGGATAGCGACAGTGGCGATGCTCTCTGCGATCTCTACGTTGAACCTGAAGCAAGCCCAGCCACCCTTCC
  TGCCACAGAGGACCCACCCTGCTTGTCCCGGCTAAAGCCGGTGTCTCCAGGCACCATCACGTGTCCTCTG
  AGAACACCGGGCAGCTTGCTGAAGGACTCTAAAATCCCTATTAGCATCAAGCATCTCTCCAACCTTCCGT
  CCAGCCATCCGGTGGTGCACCAGCAACCAGCCAGGAGTGAGGTGCCCAGAACAAAAATCCCTGTTTCCAA
  AGTGCTGGTCCGTAGGGTCAGCAACCGGGGCTTGGCTGGGACCACCATCAGGGCAGCAGCATGCCATGAC
  AGTGCCAAAAAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222762 representing NM_028113
  Red=Cloning site Green=Tags(s)
  
  MDSHCECAAETPAAEPPSGKINKAAFKLFKKRKSGGTMPSIFGVKNKGDGKSSGPTGMVRSRTHDGLAEV
  LVLEGSKKEEPPGGSDHSGARPIPGPPKPSGPGLGSLASSSVAKSHSFFSLLKKNGRSETGKGDHAEASK
  AGGKQKRGLKGIFSSMRWHRRDKRGKEEEEKAVRAAGPGNLVLPGSLTASLECVKEEPPRAARRPDSPGQ
  DASRHAAGEPAGGEQAPASAESAPERICLEAGSPTGSGDQSSRGEDAEGHRREEKPGAALESGAGEVQAA
  EDASKTGDVPIKTVPLVDSEGGSGRASAVPDPSSVDPPSDPSADRICLMFSDVTSLKSFDSLTGCGDIIA
  DPEEEAGPSCDKHVPGPGKPVLSKKNASVVAYQGGGEEMASPDQVDDTYLPEFWDMLSQTEDQGQGTQEG
  AAKAATASDIKLAPETSSDTRCGEAAKDMSSVKRRRLHRIPIESQQKEEPKHPEKEHQEGVPNSDEGYWD
  STTPGPEEESISNSSSSKKVVIPRDSDSGDALCDLYVEPEASPATLPATEDPPCLSRLKPVSPGTITCPL
  RTPGSLLKDSKIPISIKHLSNLPSSHPVVHQQPARSEVPRTKIPVSKVLVRRVSNRGLAGTTIRAAACHD
  SAKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028113
• ORF Size: 1905 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028113.3, NP_082389.1
• RefSeq Size: 2718 bp
• RefSeq ORF: 1908 bp
• Locus ID: 72125
• UniProt ID: Q8CCJ4
• Cytogenetics: 14 D1
• MW: 66.9 kDa
• Gene Summary: Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex (By similarity).[UniProtKB/Swiss-Prot Function]