Il6ra (NM_010559) Mouse Tagged ORF Clone

CAT#: MR222583

Il6ra (Myc-DDK-tagged) - Mouse interleukin 6 receptor, alpha (Il6ra)

Product Images

Circular map for MR222583

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il6ra
• Synonyms: CD126; IL-6R; IL-6R-alpha; IL-6RA; Il6r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222583 representing NM_010559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCGTCGGCTGCACGCTGTTGGTCGCCCTGCTGGCCGCGCCCGCGGTCGCGCTGGTCCTCGGGA
  GCTGCCGCGCGCTGGAGGTGGCAAATGGCACAGTGACAAGCCTGCCAGGGGCCACCGTTACCCTGATTTG
  CCCCGGGAAGGAAGCAGCAGGCAATGTTACCATTCACTGGGTGTACTCTGGCTCACAAAACAGAGAATGG
  ACTACCACAGGAAACACACTGGTTCTGAGGGACGTGCAGCTCAGCGACACTGGGGACTATTTATGCTCCC
  TGAATGATCACCTGGTGGGGACTGTGCCCTTGCTGGTGGATGTTCCCCCAGAGGAGCCCAAGCTCTCCTG
  CTTCCGGAAGAACCCCCTTGTCAACGCCATCTGTGAGTGGCGTCCGAGCAGCACCCCCTCTCCAACCACG
  AAGGCTGTGCTGTTTGCAAAGAAAATCAACACCACCAACGGGAAGAGTGACTTCCAGGTGCCCTGTCAGT
  ATTCTCAGCAGCTGAAAAGCTTCTCCTGCCAGGTGGAGATCCTGGAGGGTGACAAAGTATACCACATAGT
  GTCACTGTGCGTTGCAAACAGTGTGGGAAGCAAGTCCAGCCACAACGAAGCGTTTCACAGCTTAAAAATG
  GTGCAGCCGGATCCACCTGCCAACCTTGTGGTATCAGCCATACCTGGAAGGCCGCGCTGGCTCAAAGTCA
  GCTGGCAGCACCCTGAGACCTGGGACCCGAGTTACTACTTGCTGCAGTTCCAGCTTCGATACCGACCTGT
  ATGGTCAAAGGAGTTCACGGTGTTGCTGCTCCCGGTGGCCCAGTACCAATGCGTCATCCATGATGCCTTG
  CGAGGAGTGAAGCACGTGGTCCAGGTCCGTGGGAAGGAGGAGCTTGACCTTGGCCAGTGGAGCGAATGGT
  CCCCAGAGGTCACGGGCACTCCTTGGATAGCAGAGCCCAGGACCACCCCGGCAGGAATCCTCTGGAACCC
  CACACAGGTCTCTGTTGAAGACTCTGCCAACCACGAGGATCAGTACGAAAGTTCTACAGAAGCAACGAGT
  GTCCTCGCCCCAGTGCAAGAATCCTCGTCCATGTCCCTGCCCACATTCCTGGTAGCTGGAGGAAGCTTGG
  CGTTTGGGTTGCTTCTCTGTGTCTTCATCATCCTGAGACTCAAGCAGAAATGGAAGTCAGAGGCTGAGAA
  GGAAAGCAAGACGACCTCTCCTCCACCCCCACCGTATTCCTTGGGCCCACTGAAGCCGACCTTCCTTCTG
  GTTCCTCTCCTCACCCCACACAGCTCTGGGTCTGACAATACCGTAAACCACAGCTGCCTGGGTGTCAGGG
  ACGCACAGAGCCCTTATGACAACAGCAACAGAGACTACTTATTCCCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222583 representing NM_010559
  Red=Cloning site Green=Tags(s)
  
  MLTVGCTLLVALLAAPAVALVLGSCRALEVANGTVTSLPGATVTLICPGKEAAGNVTIHWVYSGSQNREW
  TTTGNTLVLRDVQLSDTGDYLCSLNDHLVGTVPLLVDVPPEEPKLSCFRKNPLVNAICEWRPSSTPSPTT
  KAVLFAKKINTTNGKSDFQVPCQYSQQLKSFSCQVEILEGDKVYHIVSLCVANSVGSKSSHNEAFHSLKM
  VQPDPPANLVVSAIPGRPRWLKVSWQHPETWDPSYYLLQFQLRYRPVWSKEFTVLLLPVAQYQCVIHDAL
  RGVKHVVQVRGKEELDLGQWSEWSPEVTGTPWIAEPRTTPAGILWNPTQVSVEDSANHEDQYESSTEATS
  VLAPVQESSSMSLPTFLVAGGSLAFGLLLCVFIILRLKQKWKSEAEKESKTTSPPPPPYSLGPLKPTFLL
  VPLLTPHSSGSDNTVNHSCLGVRDAQSPYDNSNRDYLFPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010559
• ORF Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010559.3, NP_034689.2
• RefSeq Size: 3343 bp
• RefSeq ORF: 1383 bp
• Locus ID: 16194
• UniProt ID: P22272
• Cytogenetics: 3 39.19 cM
• MW: 50.9 kDa
• Gene Summary: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.[UniProtKB/Swiss-Prot Function]