Gab2 (NM_010248) Mouse Tagged ORF Clone

CAT#: MR222356

Gab2 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 1

Product Images

Circular map for MR222356

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gab2
• Synonyms: AI463667; D130058I17Rik; p97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222356 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCGGCGGCGGCGACGACGTGGTGTGTACCGGCTGGCTGAGGAAATCGCCTCCCGAGAAGAAGT
  TGAGGCGCTATGCCTGGAAGAAACGCTGGTTTATACTTCGGAGTGGCCGAATGAGTGGAGATCCAGATGT
  TCTGGAATACTACAAGAATGAGCACTCCAAGAAACCCCTGCGGATCATCAACCTGAACTTGTGTGAGCAG
  GTGGATGCAGGCCTGACCTTCAACAAGAAAGAGCTGCAGGATAGTTTTGTGTTTGATATCAAGACCAGCG
  AGCGCACATTTTACCTGGTGGCTGAGACAGAGGCTGACATGAATAAGTGGGTCCAGAGCATCTGCCAGAT
  CTGCGGCTTCAATCAGGCTGAAGAGAGCACAGACTCCCTGAGGAACCTTTCTTCAGCCAGTCATGGTCCC
  CGCTCTTCTCCAGCTGAGTTCAGCTCCAGTCAGCACCTGCTCCGAGAACGGAAGTCCTCAGCCCCTTCAC
  ACTCTAGCCAGCCTACTTTATTCACGTTTGAGCCCCCTGTGTCAAGCCACATGCAGCCTACCTTGTCCAC
  CAGTGCACCTCAGGAGTATCTCTACTTGCACCAGTGCATAAGCAGAAGGACAGAAAATGCAAGGAGTGCC
  AGCTTCTCTCAGGGCACCCGGCAGAAGAGTGATACAGCTGTGCAAAAACTTGCCCAGAGCAATGGACACT
  GTATCAACGGCGTCGGAGGTCAAGTCCATGGCTTCTATAGCCTTCCCAAGCCAAGCCGACACAATACAGA
  ATTCAAAGACAGTACTTATGATCTCCCACGGAGCCTGGCTTCCCATGGCCACACCAAGAGCAGCCTCACA
  GGGTCTGAGACTGATAACGAGGATGTGTACACCTTCAAGATGCCCAGCAACACCCTGTGTCGGGAACTTG
  GAGACCTCCTTGTGGACAATATGGATGTCCCAACCACTCCTCTCTCAGCCTACCAGATCCCTAGAACTTT
  CACACTGGACAAGAACCACAATGCCATGACAGTGGCCACTCCTGGAGATTCAGCCATAGCTCCCCCACCC
  CGGCCACCCAAGCCAAGTCAGGCAGAAACACCTCAATGGGGCAGCATTCAGCAAAGACCTCCAATCAGCG
  AAAATAGCAGATCTGTAGCTGCTACTATCCCCAGGCGCAATACCCTCCCTGCAATGGACAACAGCCGACT
  CCATCGAGCTTCTTCCTGTGAGACCTACGAGTACCCGGCACGAGGCAGTGGGGAAAGTGCCAGCTGGTCT
  GCTGAACCTCCAGGAAAGACTGCCGTAGGTCGATCAAATAGTGCCAGCTCTGATGACAACTACGTGCCCA
  TGAACCCAGGTTCTTCTACCCTGCTGGCTATGGAACGACCAGGGGACAACTCCCAGAGTGTCTACATCCC
  CATGAGCCCAGGACCCCATCACTTTGACCCACTTGGCTACCCGTCCACAGCCCTTCCTATTCACAGAGGC
  CCCAGCCGAGGAAGTGAAATCCAGCCACCCCCGGTCAACCGAAACCTCAAGCCTGACAGAAAAGCAAAGC
  CAACACCCCTTGACCTGAGAAACAACACTGTCATCGATGAGCTGCCCTTCAAGTCACCTGTCACCAAGTC
  TTGGTCCAGGATCAACAGCCACACCTTTAACTCCAGTTCCTCCCAGTACTGCCGTCCTATCTCCACACAG
  AGCATCACCAGCACAGACTCGGGAGACAGTGAGGAGAACTATGTCCCTATGCAAAACCCAGTATCTGCAT
  CCCCTGTTCCCAGTGGCACTAACAGCCCAGCTCCAAAGAAGAGTACTGGCAGTGTGGATTATCTCGCCCT
  GGACTTCCAGCCGGGCTCCCCAAGCCCTCACCGCAAGCCATCCACATCATCTGTCACATCAGATGAGAAG
  GTAGACTATGTCCAAGTGGATAAAGAGAAGACCCAGGCCCTGCAGAACACCATGCAGGAGTGGACAGATG
  TGCGGCAGTCCTCCGAACCTTCCAAGGGTGCCAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222356 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGGGGDDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNEHSKKPLRIINLNLCEQ
  VDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEADMNKWVQSICQICGFNQAEESTDSLRNLSSASHGP
  RSSPAEFSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSSHMQPTLSTSAPQEYLYLHQCISRRTENARSA
  SFSQGTRQKSDTAVQKLAQSNGHCINGVGGQVHGFYSLPKPSRHNTEFKDSTYDLPRSLASHGHTKSSLT
  GSETDNEDVYTFKMPSNTLCRELGDLLVDNMDVPTTPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPP
  RPPKPSQAETPQWGSIQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPARGSGESASWS
  AEPPGKTAVGRSNSASSDDNYVPMNPGSSTLLAMERPGDNSQSVYIPMSPGPHHFDPLGYPSTALPIHRG
  PSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPVTKSWSRINSHTFNSSSSQYCRPISTQ
  SITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPGSPSPHRKPSTSSVTSDEK
  VDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010248
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010248.2, NP_034378.2
• RefSeq Size: 6008 bp
• RefSeq ORF: 2001 bp
• Locus ID: 14389
• Cytogenetics: 7 E1
• MW: 73.3 kDa