Tiam1 (NM_009384) Mouse Tagged ORF Clone

CAT#: MR222254

Tiam1 (Myc-DDK-tagged) - Mouse T-cell lymphoma invasion and metastasis 1 (Tiam1), transcript variant 1

Product Images

Circular map for MR222254

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tiam1
• Synonyms: AI847750; D16Ium10; D16Ium10e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222254 representing NM_009384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACGCAGAAAGCCAAAATGTAGACCACGAGTTTTATGGAGAAAAGCATGCCAGCCTGGGGCGGA
  AGCACACTTCACGCTCCCTGCGGCTGTCACACAAGACCCGGAGGACACGGCATGCTTCCTCCGGGAAGGC
  GATCCACAGGAACTCTGAAGTGAGCACCCGGTCCAGCAGCACGCCCAGCATCCCCCAGTCCCTGGCTGAA
  AATGGCCTGGAGCCCTTCTCCCAAGAAGGCGCCCTAGACGACTTCGGGGACCCCATCTGGGTGGACCGAG
  TAGATATGGGCTTGAGACCCGTATCTTACACCGATTCTTCCGTCACTCCCAGCGTAGATGGCAGCATCGT
  CCTCACTGCAGCCTCTGTGCAGAGCATGCCAGACTCAGAGGAGAGCCGGCTTTACGGGGATGACGCTACG
  TACTTGGCTGAGGGAGGCAGGAGGCAGTGTCCCTATACATCCAATGGGCCCACATTCATGGAGACAGCGA
  GCTTTAAGAAGAAGCGCTCCAAATCTGCAGACATCTGGCGGGAGGACAGCCTGGAATTCTCACTCTCAGA
  TCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTGGGTTCCGCGGAAGAGAAGGATTGCGAGGAG
  GCTCGGGGGATGGAAACAGAGGCGAGTCCCCGGCAGCTCAGCACCTGTCAGCGAGCCAACTCCCTGGGTG
  ACTTGTATGCTCAGAAAAACTCTGGGGTGAAGGCTAATGGAGGACCGAGGAACAGATTCTCAAGCTACTG
  CCGGAATTTGGTGTCGGATATTCCCGATCTTGCAAAGCATAAGATGCCACCGGCTGCTGCCGAAGAGACT
  CCTCCGTACAGTAATTACAACACGCTTCCCTGTAGGAAGTCGCACTGTCTTTCCGAGGGTGCCACCAACC
  CACAAATTAGCCTTAGCAAGAGCATGCAAGGCAGAAGAGCAAAAACCACCCAGGATGTTAACACAGGCGA
  GGGCAGCGAGTTTGCGGACAGTGGGATCGAAGGGGCCACCACCGACACGGACCTCTTGTCCAGGAGATCC
  AATGCCACCAACTCAAGCTACTCGCCCCCCACAGGCCGAGCCTTTGTGGGCAGCGACAGCGGCAGCAGTT
  CAACAGGGGATGCTGCGCGCCAGGGGGTGTACGAGAACTTCAGGCGGGAACTGGAGATGAGTACCACCAA
  CAGTGAGAGCCTGGAGGAGGCTGGCTCTGCGCACAGCGATGAGCAGAGCAGCGGAACCCTCAGTTCCCCG
  GGCCAGTCGGACATCCTGCTAACGGCTGCGCAGGGCACTGTGCGGAAGGCTGGAGCGCTGGCAGTCAAGA
  ACTTCCTGGTCCACAAGAAGAATAAGAAGGTGGAGTCGGCTACCCGGAGGAAGTGGAAGCACTACTGGGT
  GTCCCTGAAAGGTTGCACGCTCTTTTTCTATGAGACTGATGGCAGGTCTGGGATAGACCACAACAGTGTC
  CCCAAGCACGCCGTCTGGGTGGAGAACAGCATCGTGCAGGCTGTGCCTGAGCACCCCAAGAAAGACTTCG
  TCTTCTGCCTCAGCAACTCCCTGGGTGACGCCTTCCTCTTCCAGACCACGAGCCAGACAGAGCTGGAGAA
  CTGGATCACCGCCATCCACTCTGCCTGCGCAGCTGCCGTCGCAAGGCACCACCACAAGGAGGACACGCTC
  CGCCTCCTCAAGTCGGAAATCAAGAAGCTGGAGCAGAAGATCGACATGGACGAGAAGATGAAGAAGATGG
  GGGAGATGCAGCTGTCTTCCGTCACCGACTCGAAGAAGAAGAAGACCATCCTAGACCAGATCTTCGTTTG
  GGAGCAGAACCTCGAACAGTTCCAGATGGACCTGTTCCGATTCCGCTGTTACCTCGCCAGCCTCCAGGGC
  GGGGAGCTGCCAAACCCCAAAAGGCTGCTTGCTTTTGCGAGCCGGCCCACGAAGGTGGCCATGGGCCGCC
  TCGGGATCTTCTCCGTGTCATCTTTTCATGCCCTGGTGGCAGCCCGGACTGGTGAGATCGGAGTGAGAAG
  GCGTACTCAGGCCATGTCCAGGTCTGCAAGCAAGCGAAGGAGCAGGTTTTCTTCTCTCTGGGGCCTAGAC
  ACTACCTCCAAAAAGAAGCAGGGACGCCCAACCATCAACCAGGTGTTTGGAGAGGGGACTGATGCTGTAA
  AGAGATCTTTAGAGGGAATATTTGATGACACTGTTCCAGATGGCAAGAGGGAGAAGGAAGTGGTCTTACC
  CAGTGTCCACCAGCACAACCCCGACTGTGACATTTGGGTCCATGAATATTTCACTCCATCCTGGTTCTGT
  CTACCCAACAACCAGCCAGCCTTGACGGTTGTCCGGCCAGGGGACACTGCGAGGGACACCTTGGAGCTCA
  TTTGCAAGACACATCAACTGGATCATTCCGCCCATTACCTGCGCCTGAAATTCCTAATGGAGAACAGAGT
  GCAGTTCTACATCCCGCAGCCCGAGGAGGACATTTACGAGCTGCTTTACAAAGAAATTGAAATCTGTCCA
  AAAGTCACCCAGAATATCCACATTGAGAAGTCAGACGCGGCCGCTGATAATTACGGGTTTTTGCTTTCTT
  CTGTGGATGAAGATGGCATTCGAAGGCTCTACGTGAACAGTGTCAAGGAAACCGGGTTAGCTTCCAAGAA
  AGGCCTGAAGGCGGGGGATGAGATTCTCGAGATCAATAATCGTGCTGCTGGTACCCTGAACTCATCTATG
  CTCAAAGATTTCCTCTCACAGCCCTCCCTGGGCCTCCTGGTGAGGACCTACCCTGAGCCAGAGGGAGGCG
  TGGAGCTGCTGGAGAACCCTCCGCACCGAGTGGACGGCCCTGTGGACCTTGGCGAGAGCCCCCTCGCCTT
  CCTCACCAGCAACCCAGGGCACAGCCTCTCCAGTGAGCAGGGCAGCAGTGCTGAGACAGCTCCAGAAGAG
  GGCGAAGGACCGGACTTGGAGTCTTCTGATGAGACAGATCACAGCAGCAAGAGCACAGAACAGGTTGCAG
  CGTTTTGCCGGAGTCTGCACGAGATGAGCCCCTCTGACTCAAGCCCGTCCCCTCAGGATGCCACAAGCCC
  TCAGCTGGCAACCACACGACAGCTGTCGGATGCGGATAAGCTGCGCAAGGTGATCTGTGAACTGCTGGAG
  ACTGAACGCACCTATGTGAAAGACTTAAACTGCCTCATGGAGAGATACCTGAAGCCCCTTCAGAAGGAGA
  CCTTTCTCACCCAGGATGAGCTTGATGTACTTTTTGGAAATTTAACCGAAATGGTGGAGTTTCAAGTCGA
  GTTCCTTAAGACTCTAGAAGATGGAGTAAGACTGGTCCCTGACTTGGAAAAGCTGGAGAAGGTTGACCAG
  TTCAAGAAAGTGCTCTTCTCTCTGGGGGGATCCTTCCTGTACTACGCGGACCGCTTCAAGCTCTACAGTG
  CCTTCTGTGCGAGCCACACAAAAGTCCCCAAGGTCCTGGTGAAAGCCAAGACGGACACAGCCTTCAAGGC
  GTTCCTCGATGCCCAGAACCCGAGGCAGCAGCACTCATCCACGCTGGAGTCTTACCTCATCAAGCCCATC
  CAGAGGGTCCTCAAGTACCCGCTTCTGCTCAGGGAGCTGTTTGCGCTGACCGATGCGGAGAGCGAGGAAC
  ACTACCACCTGGACGTGGCCATCAAGACTATGAACAAGGTCGCCAGTCACATCAATGAGATGCAGAAGAT
  CCACGAAGAGTTTGGTGCTGTGTTTGACCAGCTGATTGCTGAGCAGACGGGAGAGAAGAAAGAGGTTGCA
  GATCTGAGCATGGGTGACCTGCTCTTGCACACCAGCGTCATCTGGCTGAACCCACCCGCCTCACTGGGAA
  AGTGGAAAAAGGAGCCAGAATTAGCAGCCTTCGTCTTCAAAACGGCCGTGGTCCTTGTATATAAAGACGG
  TTCCAAGCAGAAGAAGAAACTTGTTGGCTCTCACAGGCTGTCAATCTACGAGGAGTGGGACCCTTTCCGG
  TTTCGCCACATGATCCCTACCGAAGCTTTGCAGGTCCGAGCTCTGCCCAGTGCAGATGCAGAGGCAAATG
  CGGTATGCGAAATTGTCCACGTGAAATCAGAGTCAGAAGGGAGGCCCGAGCGGGTTTTCCACCTCTGCTG
  CAGTTCCCCAGAGAGCAGAAAGGACTTTCTGAAGTCTGTGCATTCGATCCTGCGAGATAAACACAGAAGA
  CAGCTCCTCAAAACGGAAAGCCTTCCGTCAGCCCAGCAGTATGTCCCCTTTGGAGGCAAGAGATTGTGTG
  CGCTTAAAGGCGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGCCCCAAGCAAGTCTCTTGGGAGGAGGAG
  GCGGCGACTGGCCCGAAACAGGTTTACCATCGATTCAGACGCCATCTCAGCCAGCAGCCCGGAGAAAGAG
  CCCCAGCAGCCCGCCGGTGGTGGGGACACTGACCGATGGGTAGAGGAACAGTTCGATCTTGCTCAGTACG
  AGGAGCAGGATGACATCAAGGAGACAGACATCCTCAGTGACGATGACGAATTCTGTGAGTCCCTGAAGGG
  CGCCTCAGTGGACAGAGACCTTCAGGAGCAGCTTCAGGCTGCCTCCATCAGTCAGCGGGCCCGAGGCCGG
  AGAACCCTCGATAGCCACGCCTCCCGCATGACACAGCTCAAGAAGCAAGCGGCCCTCTCGGGCATCAACG
  GGGGCCTGGAGAGTGCGAGCGAGGAAGTCATTTGGGTCAGGCGCGAAGACTTTGCCCCCTCCAGGAAACT
  GAACACGGAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222254 representing NM_009384
  Red=Cloning site Green=Tags(s)
  
  MGNAESQNVDHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKAIHRNSEVSTRSSSTPSIPQSLAE
  NGLEPFSQEGALDDFGDPIWVDRVDMGLRPVSYTDSSVTPSVDGSIVLTAASVQSMPDSEESRLYGDDAT
  YLAEGGRRQCPYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEE
  ARGMETEASPRQLSTCQRANSLGDLYAQKNSGVKANGGPRNRFSSYCRNLVSDIPDLAKHKMPPAAAEET
  PPYSNYNTLPCRKSHCLSEGATNPQISLSKSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRS
  NATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSP
  GQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYETDGRSGIDHNSV
  PKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTL
  RLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQG
  GELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGEIGVRRRTQAMSRSASKRRSRFSSLWGLD
  TTSKKKQGRPTINQVFGEGTDAVKRSLEGIFDDTVPDGKREKEVVLPSVHQHNPDCDIWVHEYFTPSWFC
  LPNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLMENRVQFYIPQPEEDIYELLYKEIEICP
  KVTQNIHIEKSDAAADNYGFLLSSVDEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAAGTLNSSM
  LKDFLSQPSLGLLVRTYPEPEGGVELLENPPHRVDGPVDLGESPLAFLTSNPGHSLSSEQGSSAETAPEE
  GEGPDLESSDETDHSSKSTEQVAAFCRSLHEMSPSDSSPSPQDATSPQLATTRQLSDADKLRKVICELLE
  TERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQ
  FKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPRQQHSSTLESYLIKPI
  QRVLKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVA
  DLSMGDLLLHTSVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRLSIYEEWDPFR
  FRHMIPTEALQVRALPSADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKSVHSILRDKHRR
  QLLKTESLPSAQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKE
  PQQPAGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESLKGASVDRDLQEQLQAASISQRARGR
  RTLDSHASRMTQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009384
• ORF Size: 4773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009384.3, NP_033410.2
• RefSeq Size: 7312 bp
• RefSeq ORF: 4776 bp
• Locus ID: 21844
• Cytogenetics: 16 51.5 cM
• MW: 177.9 kDa