Adgrg2 (NM_001079857) Mouse Tagged ORF Clone

CAT#: MR222121

Gpr64 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 64 (Gpr64), transcript variant 2

Product Images

Circular map for MR222121

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adgrg2
• Synonyms: AW212196; B830041D06Rik; Gpr64; Me6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222121 representing NM_001079857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTCTGGTGGGCAGTACAGCCCTGTTGGCAGACCTGAAGAGGTTTTACTGATATACAAGATAT
  TCCTTGTCATCATTTGTTTTCATGTCATTCTCGTTACATCCCTGAAAGAAAACGGTAATTCCAGTTTGTT
  ATCACCATCTGCTGAATCATCTCTTGTCAGTCTCATCCCCTACTCCAATGGTACACCAGATGCTGCTTCA
  GAAGTTTTGTCGACTTTAAACAAAACAGGAGTCAAATCCCAGAGAAATATCTGCAATTTGTCATCTCTTT
  GCAATGACTCAGTATTTTTTAGAGGTGAGATAGTGTTTCAACATGATGAAGACCACAATGTTACCCAGAA
  TCAAGATACAGCTAATGGCACCTTCGCTGGAGTCCTGTCTCTAAGTGAACTGAAGCGATCAGAGCTCAAC
  AAAACTCTACAGACCTTAAGTGAGACTTACTTTATAGTGTGTGCTACAGCAGAGGCCCAAAGCACGGTAA
  ACTGTACATTCACAGTAAAACTCAATGAGACCATGAATGTGTGTGCCATGATGGTTACTTTCCAAACTGT
  ACAGATTCGGCCAATGGAACAGTGCTGCTGTTCCCCGAGGACTCCCTGCCCTTCCTCACCAGAAGAGTTA
  GAAAAACTACAGTGTGAACTGCAGGATCCCATTGTCTGTCTTGCTGATCAACCGCATGGCCCACCGTTAT
  CGTCTTCCAGCAAGCCTGTTGTACCTCAGGCCACCATTATTTCCCATGTTGCTAGTGACTTCTCTTTGGC
  TGAACCCCTTGATCATGCCCTTATGACCCCAAGCACACCCTCTCTGACACAAGAAAGTAACCTTCCATCT
  CCTCAGCCTACGATCCCCCTGGCTTCCAGTCCTGCCACTGACTTGCCAGTTCAATCTGTAGTGGTCTCTT
  CTTTGCCTCAAACTGATCTTTCCCACACCCTGTCACCGGTGCAGTCCTCCATTCCCTCTCCTACCACACC
  AGCCCCATCTGTCCCTACAGAACTGGTCACCATCAGCACACCTCCTGGTGAGACAGTTGTCAACACTAGC
  ACTGTTTCTGATCTGGAAGCCCAAGTATCCCAGATGGAGAAAGCTTTGTCTTTGGGTAGCTTAGAGCCTA
  ATCTTGCAGGCGAAATGGTAAACCGAGTCAGCAAACTCCTTCACTCTCCACCTGCCTTGCTAGCCCCTCT
  AGCTCAAAGGTTGCTAAAAGTGGTAGATGCCATTGGCTTACAGCTGAATTTTTCATCTACAACTATCAGT
  CTAACTTCACCTTCTTTGGCTCTTGCTGTGATCAGAGTGAATGCCAGTAATTTCAATACCACGACTTTTG
  CAGCCCAAGACCCAACAAATCTCCAGGTTTCTCTGGAAACCCCACCTCCTGAGAATAGTATTGGTGCCAT
  TACTCTGCCCTCATCACTGATGAATAATTTGCCAGCTAATGATGTAGAATTGGCTTCAAGGATTCAGTTC
  AATTTCTTTGAAACACCCGCCCTGTTTCAGGATCCTTCCCTGGAGAACCTCACTCTGATAAGCTATGTCA
  TATCATCAAGTGTCACAAACATGACCATCAAGAACTTGACAAGAAACGTGACAGTCGCACTGAAACACAT
  CAACCCAAGTCCGGATGACTTAACTGTGAAATGTGTATTCTGGGACTTGGGCAGAAATGGTGGCAAAGGA
  GGTTGGTCATCTGATGGCTGTTCCGTCAAAGACAAGAGAATGAATGAAACCATCTGTACCTGTAGCCATC
  TTACAAGTTTTGGCATCCTATTGGACCTATCTCGAACATCCTTACCACCAAGTCAAATGATGGCTCTGAC
  ATTTATCACGTATATTGGCTGTGGGCTTTCATCAATTTTTCTGTCAGTTACTCTTGTAACCTACATCGCC
  TTTGAAAAGATCCGGAGGGATTACCCCTCCAAAATCCTCATCCAGCTGTGTGCTGCCCTGCTTCTGCTCA
  ACCTGATCTTCCTCCTAGACTCCTGGATTGCGCTGTATAATACCCGAGGTTTCTGCATTGCCGTGGCTGT
  ATTTCTTCACTATTTTCTCTTGGTCTCATTCACATGGATGGGATTAGAAGCATTCCACATGTACCTAGCA
  CTGGTCAAGGTGTTTAATACTTACATCCGAAAGTACATCCTTAAATTCTGCATTGTTGGCTGGGGCATAC
  CAGCTGTGGTTGTGTCCATCGTCCTGACTATATCCCCAGATAACTATGGGATTGGATCCTATGGAAAATT
  CCCCAATGGCACACCAGATGACTTTTGCTGGATCAACAGCAATGTGGTGTTCTATATCACGGTTGTGGGA
  TATTTCTGTGTGATATTTCTACTGAACGTCAGCATGTTCATCGTGGTCTTGGTTCAGCTCTGTCGAATTA
  AAAAGAAGAAGCAGCTGGGAGCCCAGCGCAAAACTAGTATTCAAGACCTCAGGAGTATCGCTGGCCTCAC
  ATTTTTACTGGGAATTACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAATGTCACCTTCATGTAT
  CTCTTTGCCATCTTTAACACCTTACAAGGGTTTTTCATATTCATTTTTTACTGTGCAGCAAAAGAGAATG
  TCAGAAAACAGTGGAGGCGGTATCTTTGTTGTGGAAAATTACGGCTGGCTGAAAATTCTGACTGGAGTAA
  AACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTATCCAGCTCTTCAAATTCCTTACAG
  TCAAGCTGTAACTCCACTAACTCCACCACACTCCTAGTGAATAGTGATTGCTCAGTGCACGCAAGCGGGA
  ATGGCAATGCATCTACCGAGAGGAATGGGGTTTCTTTCAGTGTTCAGAATGGAGACGTGTGCCTTCATGA
  TCTCACTGGCAAACAGCATATGTTTAGTGACAAAGAGGACTCCTGCAATGGTAAAAGCCGGATAGCGCTC
  AGAAGGACTTCGAAGCGGGGAAGCTTACACTTTATCGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222121 representing NM_001079857
  Red=Cloning site Green=Tags(s)
  
  MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHVILVTSLKENGNSSLLSPSAESSLVSLIPYSNGTPDAAS
  EVLSTLNKTGVKSQRNICNLSSLCNDSVFFRGEIVFQHDEDHNVTQNQDTANGTFAGVLSLSELKRSELN
  KTLQTLSETYFIVCATAEAQSTVNCTFTVKLNETMNVCAMMVTFQTVQIRPMEQCCCSPRTPCPSSPEEL
  EKLQCELQDPIVCLADQPHGPPLSSSSKPVVPQATIISHVASDFSLAEPLDHALMTPSTPSLTQESNLPS
  PQPTIPLASSPATDLPVQSVVVSSLPQTDLSHTLSPVQSSIPSPTTPAPSVPTELVTISTPPGETVVNTS
  TVSDLEAQVSQMEKALSLGSLEPNLAGEMVNRVSKLLHSPPALLAPLAQRLLKVVDAIGLQLNFSSTTIS
  LTSPSLALAVIRVNASNFNTTTFAAQDPTNLQVSLETPPPENSIGAITLPSSLMNNLPANDVELASRIQF
  NFFETPALFQDPSLENLTLISYVISSSVTNMTIKNLTRNVTVALKHINPSPDDLTVKCVFWDLGRNGGKG
  GWSSDGCSVKDKRMNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSIFLSVTLVTYIA
  FEKIRRDYPSKILIQLCAALLLLNLIFLLDSWIALYNTRGFCIAVAVFLHYFLLVSFTWMGLEAFHMYLA
  LVKVFNTYIRKYILKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWINSNVVFYITVVG
  YFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNVTFMY
  LFAIFNTLQGFFIFIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNSLQ
  SSCNSTNSTTLLVNSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKEDSCNGKSRIAL
  RRTSKRGSLHFIEQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079857
• ORF Size: 2985 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079857.2, NP_001073326.1
• RefSeq Size: 4685 bp
• RefSeq ORF: 2988 bp
• Locus ID: 237175
• UniProt ID: Q8CJ12
• Cytogenetics: X F4
• MW: 109.1 kDa
• Gene Summary: Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.[UniProtKB/Swiss-Prot Function]