Dusp4 (NM_176933) Mouse Tagged ORF Clone

CAT#: MR222119

Dusp4 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 4 (Dusp4)

Product Images

Circular map for MR222119

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dusp4
• Synonyms: 2700078F24Rik; AI844617; BB104621; E130306H24Rik; MKP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222119 representing NM_176933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACGATGGAGGAACTGCGGGAGATGGACTGCAGCGTGCTCAAAAGGCTGATGAACCGGGATGAGA
  ACGGCGGCGGCGGCAGCGCGGGCGGCAACGGCAGCGGCAGCCACGGCGCCTTGGGGCTGCTGAGCGGCGG
  CAAGTGCCTGCTGCTGGACTGCAGGCCGTTTCTGGCTCACAGCGCGGGCTACATCCGAGGCTCGGTGAAC
  GTGCGCTGCAATACCATCGTGCGGCGGAGGGCCAAGGGCTCCGTGAGCCTGGAGCAGATCCTGCCCGCCG
  AGGAAGAGGTGCGTGCCCGCCTGCGCTCTGGCCTCTACTCGGCTGTCATCGTCTACGACGAGCGCAGCCC
  GCGCGCCGAGAGTCTCCGGGAGGACAGCACGGTGTCGCTGGTCGTGCAGGCGTTGCGCCGGAACGCGGAG
  CGCACGGACATCTGCCTGCTTAAAGGTGGCTATGAGAGGTTTTCTTCTGAATACCCAGAATTCTGCTCTA
  AAACCAAGGCCCTGGCCGCCATCCCACCCCCTGTACCTCCCAGCACCAATGAGTCCTTGGACCTGGGCTG
  CAGCTCCTGTGGAACCCCGCTGCACGACCAGGGGGGCCCCGTGGAAATCCTCCCTTTCCTCTACCTCGGC
  AGTGCCTACCATGCTGCCCGCAGGGACATGTTGGATGCCCTGGGGATCACGGCCCTCCTGAATGTCTCTT
  CAGACTGTCCCAATCACTTTGAGGGTCATTACCAGTACAAGTGCATCCCCGTCGAAGACAACCACAAGGC
  CGACATCAGCTCCTGGTTCATGGAAGCCATCGAGTACATCGACGCAGTAAAGGACTGTCGAGGGCGAGTG
  CTGGTTCACTGCCAGGCCGGCATCTCCCGATCAGCCACCATCTGCCTGGCCTACCTGATGATGAAGAAGC
  GGGTGAGGCTGGAGGAGGCTTTTGAGTTCGTCAAGCAGCGCCGAAGCATCATCTCGCCCAACTTCAGCTT
  CATGGGCCAGTTGCTGCAGTTTGAGTCTCAGGTGCTCACCACGTCCTGCGCCGCCGAGGCTGCCAGCCCT
  TCTGGGCCCCTTCGGGAGAGGGGAAAGGCCACCCCGACCCCCACCTCGCAGTTCGTCTTCAGCTTCCCTG
  TGTCTGTGGGCGTGCACGCGGCCCCCAGTAACCTGCCGTACCTGCACAGCCCCATCACCACCTCCCCCAG
  CTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222119 representing NM_176933
  Red=Cloning site Green=Tags(s)
  
  MVTMEELREMDCSVLKRLMNRDENGGGGSAGGNGSGSHGALGLLSGGKCLLLDCRPFLAHSAGYIRGSVN
  VRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAE
  RTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSTNESLDLGCSSCGTPLHDQGGPVEILPFLYLG
  SAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRV
  LVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLTTSCAAEAASP
  SGPLRERGKATPTPTSQFVFSFPVSVGVHAAPSNLPYLHSPITTSPSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176933
• ORF Size: 1197 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176933.4, NP_795907.1
• RefSeq Size: 2427 bp
• RefSeq ORF: 1197 bp
• Locus ID: 319520
• UniProt ID: Q8BFV3
• Cytogenetics: 8 A4
• MW: 43.4 kDa
• Gene Summary: Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.[UniProtKB/Swiss-Prot Function]