Glra1 (NM_020492) Mouse Tagged ORF Clone

CAT#: MR222118

  • TrueORF®

Glra1 (Myc-DDK-tagged) - Mouse glycine receptor, alpha 1 subunit (Glra1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_020492" in other vectors (4)


Interest in protein/lysate? Submit request here!

Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Rabbit Anti-Glycine Receptor Antibody
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Other products for "Glra1"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Glra1
Synonyms nmf11; oscillator; ot; spasmodic; spd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR222118 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACAGCTTCAATACTCTGCGATTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCCA
AAGAGGCTGAAGCCGCCCGCTCCGCACCCAAGCCTATGTCACCCTCGGACTTCCTGGATAAGCTCATGGG
GAGGACTTCTGGGTATGACGCCAGGATCAGGCCCAACTTTAAAGGTCCTCCTGTGAACGTAAGTTGCAAC
ATCTTCATCAACAGTTTCGGTTCCATCGCTGAGACAACCATGGACTATAGGGTCAACATCTTCCTGAGGC
AGCAGTGGAACGACCCCCGTCTGGCCTACAATGAATACCCTGATGACTCTCTGGACCTTGACCCATCTAT
GTTGGATTCCATCTGGAAGCCTGACTTGTTCTTTGCCAATGAGAAGGGGGCCCACTTCCACGAAATCACC
ACGGACAACAAACTGCTAAGAATCTCCCGGAATGGCAATGTCCTCTACAGCATCAGAATCACCCTGACGC
TGGCCTGCCCCATGGACCTGAAGAATTTCCCGATGGATGTACAGACGTGTATCATGCAACTCGAAAGCTT
TGGATATACCATGAACGACCTCATCTTTGAGTGGCAGGAGCAAGGAGCTGTGCAGGTGGCAGACGGACTG
ACCCTGCCTCAGTTTATTCTGAAGGAAGAGAAAGACCTGAGATACTGCACCAAGCACTACAACACAGGTA
AATTCACCTGCATCGAGGCCCGATTCCACCTGGAGCGGCAGATGGGCTACTACCTGATCCAGATGTACAT
CCCCAGCCTGCTCATCGTCATCCTGTCCTGGATCTCCTTCTGGATCAACATGGATGCTGCACCAGCTCGT
GTGGGGCTGGGCATCACCACAGTGCTCACCATGACCACACAGAGCTCTGGCTCCCGAGCCTCCCTACCCA
AGGTGTCCTACGTGAAAGCTATTGACATTTGGATGGCTGTTTGCCTGCTCTTCGTGTTCTCTGCCCTGCT
GGAGTACGCCGCCGTCAACTTTGTGTCTCGGCAACACAAGGAACTGCTTCGATTTAGGAGGAAGAGGCGA
CATCACAAGGATGATGAGGGTGGAGAAGGCCGCTTCAACTTCTCTGCCTATGGGATGGGCCCAGCCTGTC
TGCAGGCCAAGGATGGCATCTCTGTCAAGGGTGCCAACAACAACAACACCACTAACCCGCCTCCTGCGCC
ATCCAAGTCCCCGGAGGAGATGCGGAAACTCTTCATCCAGAGAGCCAAGAAGATCGACAAGATATCTCGC
ATCGGTTTCCCCATGGCCTTCCTCATCTTCAACATGTTCTACTGGATCATCTATAAGATCGTCCGGAGAG
AGGATGTCCACAACAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR222118 protein sequence
Red=Cloning site Green=Tags(s)

MYSFNTLRFYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCN
IFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEIT
TDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGL
TLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPAR
VGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRR
HHKDDEGGEGRFNFSAYGMGPACLQAKDGISVKGANNNNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISR
IGFPMAFLIFNMFYWIIYKIVRREDVHNK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020492
ORF Size 1350 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020492.2
RefSeq Size 2389 bp
RefSeq ORF 1350 bp
Locus ID 14654
Cytogenetics 11 33.12 cM
MW 51.7 kDa
Gene Summary Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel opening is also triggered by taurine and beta-alanine (By similarity). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization (By similarity). Plays an important role in the down-regulation of neuronal excitability (PubMed:9145798). Contributes to the generation of inhibitory postsynaptic currents (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (PubMed:24801766).[UniProtKB/Swiss-Prot Function]

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