Pdia2 (NM_001081070) Mouse Tagged ORF Clone

CAT#: MR221945

Pdia2 (Myc-DDK-tagged) - Mouse protein disulfide isomerase associated 2 (Pdia2)

Product Images

Circular map for MR221945

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdia2
• Synonyms: 1810041F13Rik; AI661267; Pdip; Pdipl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221945 representing NM_001081070
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGCAGCTTCTGCCAGTGTTGCTGCTCCTGCTGGGTGTCTCAGGCTCATGGGGACAGGGAGAAG
  AGCCTGGGGGTCCTTCAGAGGTGCTCCCTGAGGAGCCCACTGGGGAAGAAGTCCCCAAGGAGGATGGTAT
  CTTGGTCTTGAACCACCGAACCCTGAGCCTGGCACTGCAGGAGCATTCAGCCCTGATGGTGGAGTTTTAT
  GCCCCATGGTGTGGTCACTGCAAAGAACTAGCTCCCGAGTACAGCAAGGCGGCTGCCCTGCTGGCGGCTG
  AGTCAGCTGTGGTCACCTTGGCCAAGGTGGATGGCCCTGCGGAACCGGAGCTGACCAAGGAGTTTGAAGT
  GGTAGGGTACCCTACACTCAAGTTCTTCCAGAATGGAAACCGCACAAACCCAGAAGAGTACGCAGGGCCC
  AAGACTGCTGAGGGCATTGCTGAGTGGCTGAGGCGGCGAGTGGGGCCCAGTGCCACACATCTGGAGGATG
  AGGAAGGTGTCCAGGCACTGATGGCCAAATGGGATATGGTGGTCATTGGCTTCTTTCAGGATCTACAGGG
  AAAGGATATGGCAACTTTCCTGGCCTTGGCCAAGGATGCCCTGGACATGACCTTTGGCTTCACTGACCAG
  CCACAACTTTTTGAGAAGTTTGGCCTCACCAAGGATACTGTGGTCCTTTTCAAGAAGTTTGATGAGGGTC
  GGGCCGACTTCCCGGTAGACAAGGAGACTGGCCTGGACCTGGGGGACCTGTCCCGCTTCCTCGTCATACA
  TAGCATGCACCTGGTCACAGAATTCAACAGCCAGACATCACCTAAGATCTTTGCAGCCAAGATCCTCAAC
  CACCTGCTGCTGTTTGTGAACCAGACACTAGCTCAACACAGGGAACTGCTGACAGACTTCAGAGAGGCAG
  CTCCTCCATTCAGGGGGCAGGTGTTGTTCGTAATGGTGGATGTGGCTGCTGACAACAGCCATGTGCTGAA
  TTATTTTGGCCTCAAGGCTGAGGAAGCCCCCACCCTGCGTCTGATCAATGTTGAGACTACCAAGAAGTAT
  GCACCTACAGGGGTGATAGCCATCACTGCAGCTTCTGTGGCTGCCTTCTGCCAGGCAGTTCTCCATGGGG
  AAATCAAGCACTATCTCCTGAGTCAGGAGATACCCCCTGACTGGGACCAGGGGCCAGTCAAGACTCTCGT
  GAGCAAGAATTTTGAGCAGGTTGCCTTTGATGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCATGG
  TGCAGCCACTGCAAGGAGATGGCTCCAGCCTGGGAGGCATTGGCAGAGAAGTACAAGGATCGTGAGGACA
  TTGTCATTGCCGAGTTGGATGCTACAGCTAATGAGCTGGAGGCCTTCTCTGTGCTCGGCTATCCCACTCT
  CAAGTTCTTTCCTGCAGGGCCTGACCGAAAGGTTATAGACTACAAAAGCACTAGGGACCTAGAGACATTC
  TCCAAGTTCCTGGACAGCGGAGGTCACCTGCCTAAGGAGGAACCCAAGGAGCCTGCAGCCTCTGCCCCAG
  AAGCACAAGCCAATTCCACCTTGGGTCCCAAGGAGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221945 representing NM_001081070
  Red=Cloning site Green=Tags(s)
  
  MDKQLLPVLLLLLGVSGSWGQGEEPGGPSEVLPEEPTGEEVPKEDGILVLNHRTLSLALQEHSALMVEFY
  APWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGP
  KTAEGIAEWLRRRVGPSATHLEDEEGVQALMAKWDMVVIGFFQDLQGKDMATFLALAKDALDMTFGFTDQ
  PQLFEKFGLTKDTVVLFKKFDEGRADFPVDKETGLDLGDLSRFLVIHSMHLVTEFNSQTSPKIFAAKILN
  HLLLFVNQTLAQHRELLTDFREAAPPFRGQVLFVMVDVAADNSHVLNYFGLKAEEAPTLRLINVETTKKY
  APTGVIAITAASVAAFCQAVLHGEIKHYLLSQEIPPDWDQGPVKTLVSKNFEQVAFDETKNVFVKFYAPW
  CSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFFPAGPDRKVIDYKSTRDLETF
  SKFLDSGGHLPKEEPKEPAASAPEAQANSTLGPKEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081070
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081070.1, NP_001074539.1
• RefSeq Size: 1721 bp
• RefSeq ORF: 1584 bp
• Locus ID: 69191
• UniProt ID: D3Z6P0
• Cytogenetics: 17 A3.3
• MW: 58.8 kDa
• Gene Summary: Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins (By similarity).[UniProtKB/Swiss-Prot Function]