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Myh3 (NM_001099635) Mouse Tagged ORF Clone
CAT#: MR221574
- TrueORF®
Myh3 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3)
ORF Plasmid: tGFP
"NM_001099635" in other vectors (2)
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Frequently bought together (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Myh3 |
| Synonyms | MyHC-emb; Myhs; Myhs-; Myhs-e; Myhse |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221574 representing NM_001099635
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCGACACCGAGATGGAAGTGTTTGGCATAGCTGCACCTTTCCTCCGCAAGTCGGAAAAGGAGA GGATCGAAGCTCAGAACCAGCCCTTTGATGCCAAAACCTACTGCTTTGTGGTTGACTCAAAGGAAGAATA TGTCAAGGGGAAAATTAAGAGTAGCCAGGATGGGAAAGTCACTGTGGAGACAGAAGACAGCAGGACCCTG GTGGTTAAGCCAGAGGACGTGTATGCCATGAACCCACCCAAGTTTGACAAGATTGAGGACATGGCCATGC TGACCCACCTGAACGAGCCCGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTACAC CTATTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAATGGCTGCCAGTGTACAACCCCGAGGTGGTG GACGGCTACCGAGGCAAAAAACGCCAGGAGGCTCCGCCCCACATCTTCTCCATCTCCGACAACGCCTACC AGTTCATGCTGACTGATCGTGAGAACCAGTCTATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACGGT GAACACCAAGCGGGTCATCCAGTACTTTGCAACAATTGCAGCCACTGGGGACCTTGCCAAGAAGAAGGAC TCCAAAATGAAGGGGACGCTGGAGGATCAAATTATCAGCGCTAACCCCCTGCTGGAGGCCTTTGGGAACG CCAAGACCGTGAGGAACGACAACTCGTCTCGCTTTGGCAAGTTCATCCGCATCCATTTTGGCACCACTGG GAAGCTGGCTTCTGCAGATATTGAAACTTATCTGCTGGAAAAGTCCAGAGTCACCTTCCAGCTGAAGGCT GAGAGAAGCTACCACATCTTCTACCAGATTCTCTCCAACAAGAAACCTGAGCTGATTGAGCTGCTGCTGA TTACGACCAACCCTTATGACTACCCGTTCATCAGCCAGGGCGAAATCCTGGTGGCCAGCATCGACGATGC GGAGGAGCTGTTAGCTACGGATAGTGCCATCGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTT TATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CCGAGCCAGATGGCACTGAAGTGGCTGACAAAACAGCCTACTTGATGGGCCTGAATTCCTCAGACCTCCT CAAGGCCTTGTGCTTTCCCAGAGTGAAAGTCGGGAATGAATACGTTACAAAAGGGCAGACTGTGGACCAG GTTCACCATGCTGTGAACGCTCTCTCCAAGTCCGTGTACGAGAAGCTGTTCTTGTGGATGGTCACTCGTA TTAACCAGCAGCTGGATACAAAGCTACCAAGACAGCACTTCATTGGCGTTTTGGACATTGCGGGTTTTGA AATTTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAGTTTTTC AACCACCACATGTTCGTGCTGGAACAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCATCGACT TCGGGATGGACCTGGCAGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCTGGAAGA GGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTGGGAAAG TCCAACAACTTCCAGAAACCTAAGGTGGTCAAAGGCAAGGCTGAAGCCCACTTCTCCCTGGTGCACTATG CGGGCACCGTGGACTACAGTGTCTCAGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGT GGGACTGTACCAGAAGTCCTCCAACAGACTCCTGGCACATCTCTATGCCACCTTCGCTACAACAGATGCG GACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACCGTCTCTGCACTGTTCAGGG AAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACACACCCACACTTTGTACGCTGTATAATACCCAA CGAAACCAAGACCCCAGGGGCCATGGAACACAGCCTTGTCCTGCACCAGCTCAGGTGTAACGGGGTGCTG GAAGGCATCCGGATCTGCAGGAAGGGCTTCCCCAACAGGATCCTGTATGGAGACTTTAAACAGAGATACC GAGTGCTGAACGCCAGTGCCATCCCAGAAGGCCAGTTCATTGACAGCAAGAAAGCTTGCGAGAAACTCTT GGCTTCCATTGACATCGACCACACACAGTACAAATTTGGACACACCAAGGTGTTCTTTAAGGCTGGCTTG CTAGGAACCCTGGAAGAGATGCGGGATGAGCGTTTGGCCAAACTGATCACTCGGACACAAGCCGTGTGCA GAGGGTTTCTCATGCGTGTGGAATTCCAGAAGATGATGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTA CAACATCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTG CTGAAGAGCGCGGAGACTGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGGATGAGC TGGCTAAGTCGGAGGCAAAGAGGAAGGAGCTAGAGGAGAAGCTCGTCACTTTGGTACAGGAGAAGAATGA CCTGCAACTTCAAGTACAGGCTGAGAGTGAAAATTTGTTGGATGCTGAGGAACGATGCGACCAGCTGATC AAAGCCAAGTTCCAGCTGGAAGCCAAAATCAAAGAGGTGACAGAGAGAGCCGAGGACGAGGAGGAGATCA ACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCGGAGCTGAAGAAGGACATCGATGA CCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACAGAGAACAAGGTCAAAAACCTTACC GAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCC ACCAGCAGACCCTGGACGACCTCCAGGCTGAAGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAA GCTGGAGCAGCAAGTGGATGATCTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAA AGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACA AGCAACAGCTAGACGAGAGGCTCAAGAAGAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGA TGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAGGCTCGCATCGAGGAGCTGGAG GAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGC TGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAA GCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTG GCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGG TGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGA GAGCGTGTCCAAATCCAAGGCCAATCTGGAGAAGATCTGCCGAACTCTAGAGGATCAGTTAAGTGAGGCC AGGGGCAAGAATGAGGAGATGCAGAGGAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAG AGGCGGGTGAGCTGAGTCGTCAACTAGAAGAAAAGGAAAGCATTGTATCTCAGCTCTCCAGGAGCAAGCA GGCCTTTACCCAACAGATAGAGGAGCTCAAGAGGCAACTGGAGGAAGAGAACAAGGCCAAGAACGCGCTG GCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTACGAGGAGGAGCAGGAAG GCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAGTATGA AACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAAAAAAAGCTTGCGCAGCGCCTTCAGGAT TCGGAGGAGCAGGTAGAGGCTGTAAATGCCAAATGTGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAG GGGAGGTGGAGGACCTGATGGTGGATGTGGAAAGAGCCAACTCCCTGGCTGCCGCTCTGGACAAGAAGCA GAGGAACTTTGACAAGGTGCTAGCTGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCCGAGCTGGAGGCA GCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAGAATGCCTATGAAGAAGCCTTGG ATCAACTTGAAACCGTGAAACGGGAGAATAAGAACTTAGAACAAGAGATAGCAGATCTCACTGAACAGAT TGCCGAGAACGGTAAAAGCATCCATGAGCTAGAGAAATCCAGAAAACAGATGGAGCTGGAGAAGGCTGAC ATCCAGATGGCGCTGGAGGAAGCAGAGGCAGCTCTTGAGCACGAAGAAGCCAAGATTCTCCGGATCCAGC TTGAACTGACCCAGGTGAAATCAGAGATTGATAGGAAGATTGCAGAGAAGGATGAAGAGATCGAGCAGCT GAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAAGGCGCCCTGGACGCTGAGGTGCGCAGCAGGAAT GAGGCCATCCGCCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATCGAGATCCAGCTGAGCCACGCCA ACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGCGTCCAGGGACAGCTGAAGGACACCCAGCTGCA CCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATCGTGGAACGCAGAGCCAAC CTCCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAG AGCAGGAGCTGTTGGATTCGAATGAGAGGGTCCAGCTGCTGCACACCCAGAACACAAGCCTCATCCACAC CAAGAAGAAGTTGGAGACTGACCTCACGCAGCTCCAGAGTGAGGTGGAGGATGCCTGCAGGGACGCAAGG AATGCAGAGGAGAAGGCCAAAAAGGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGAAGAAGGAGC AGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCG TCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATTCAGAAACTGGAGACACGGATC AGAGAGCTGGAGTTTGAGCTGGAAGGGGAGCAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGT ATGAGCGGCGTGTTAAGGAGCTCACATATCAGAGTGAGGAGGACAGGAAGAATGTGTTGAGATTGCAGGA TCTGGTGGATAAACTCCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCTGATGAACAAGCC AATGCTCATCTCACCAAGTTCAGGAAAGCCCAGCATGAGCTAGAGGAGGCCGAGGAACGGGCTGATATCG CAGAATCGCAAGTCAATAAACTGCGGGCAAAGACCCGTGACTTCACCTCTAGCCGGATGGTGGTCCATGA AAGCGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221574 representing NM_001099635
Red=Cloning site Green=Tags(s) MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYVKGKIKSSQDGKVTVETEDSRTL VVKPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVV DGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKA ERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGL YKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQ VHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGK SNNFQKPKVVKGKAEAHFSLVHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATTDA DGGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVL EGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGL LGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPL LKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLI KAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLT EELAGLDETIAKLTREKKALQEAHQQTLDDLQAEEDKVNSLSKLKSKLEQQVDDLESSLEQEKKLRVDLE RNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQTLSLQLQKKIKELQARIEELE EEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATV ATLRKKHADSAAELAEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSVESVSKSKANLEKICRTLEDQLSEA RGKNEEMQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQIEELKRQLEEENKAKNAL AHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQD SEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEA ALKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQMELEKAD IQMALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQGALDAEVRSRN EAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRAN LLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLTQLQSEVEDACRDAR NAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRI RELEFELEGEQKRNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQA NAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001099635 |
| ORF Size | 5820 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001099635.1, NP_001093105.1 |
| RefSeq Size | 5992 bp |
| RefSeq ORF | 5823 bp |
| Locus ID | 17883 |
| UniProt ID | P13541 |
| Cytogenetics | 11 40.59 cM |
| MW | 224.2 kDa |
| Gene Summary | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. [provided by RefSeq, Sep 2015] |
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