Ampd1 (NM_001033303) Mouse Tagged ORF Clone

CAT#: MR220907

Ampd1 (Myc-DDK-tagged) - Mouse adenosine monophosphate deaminase 1 (Ampd1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ampd1
• Synonyms: AI553520; Ampd-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220907 representing NM_001033303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTATTCAAACTAACAGGTCAAGGAAAACAAATTGATGATGCAATGCGTAGCTTTGCTGAAAAAG
  TGTTTGCCTCTGAAGTCAAAGATGAGGGAGGTCGGCACGAGATCTCCCCCTTCGACGTGGATGAGATCTG
  CCCAATTTCCCTTCATGAGATGCAGGCCCACATATTCCACATGGAGAACCTGTCCATGGATGGCAGGAGA
  AAAAGGCGTTTCCAAGGACGGAAGACTGTTAATTTGTCCATTCCACAAAGTGAAACATCTTCTACCAAAC
  TGTCCCACATCGAAGAATTTATTTCTTCATCCCCAACCTACGAGAGTGTGCCTGACTTCCAGAGGGTGCA
  GATCACTGGAGACTATGCCTCGGGGGTAACCGTTGAAGACTTTGAGGTGGTTTGTAAAGGTCTCTATCGG
  GCATTGTGTATACGAGAGAAATACATGCAGAAGTCATTCCAGAGGTTCCCCAAGACCCCCTCCAAGTACT
  TGAGGAACATTGATGGCGAAGCCTTGGTAGGAAACGAGAGCTTCTACCCAGTATTTACCCCTCCTCCGAA
  GAAGGGTGAAGACCCCTTTCGCACAGAGGACCTTCCTGCAAACCTGGGCTATCACCTCAAGATGAAAGCT
  GGTGTGATTTACATCTACCCTGATGAAGCGGCAGCCAACAGAGATGAGCCCAAGCCCTACCCTTACCCAA
  ATCTGGATGACTTCCTGGATGACATGAATTTTTTGCTTGCTCTAATTGCACAAGGGCCTGTTAAGACTTA
  TGCTCACCGCCGCCTGAAGTTCCTCTCCTCCAAGTTCCAGGTCCATCAGATGCTCAACGAGATGGATGAG
  CTGAAGGAGCTGAAGAACAACCCCCACCGGGACTTTTATAACTGCAGGAAGGTGGATACTCACATCCATG
  CAGCTGCCTGCATGAACCAGAAGCACCTGCTGCGCTTCATTAAGAAATCTTACCATATTGACGCCGACAG
  GGTGGTCTACAGCACTAAAGAGAAGAGCCTGACTCTGAAGGAACTTTTCGCTAAGTTGAATATGCATCCC
  TATGACCTGACTGTTGACTCTCTGGATGTTCATGCTGGACGCCAGACGTTCCAACGTTTTGATAAGTTCA
  ATGACAAATACAATCCCGTGGGTGCAAGTGAGCTTCGGGACCTCTACCTAAAAACAGACAACTACATTAA
  TGGGGAGTATTTTGCCACTATCATCAAGGAGGTGGGTGCAGACCTGGTGGAGGCCAAGTATCAGCATGCA
  GAGCCTCGCTTATCCATCTACGGTCGCAGTCCAGATGAGTGGAACAAACTCTCCTCTTGGTTTGTCTGCA
  ATCGCATCTATTGCCCCAACATGACGTGGATGATCCAGGTCCCCAGGATCTATGATGTGTTCCGATCCAA
  GAATTTCCTGCCACACTTCGGAAAGATGCTGGAGAATATTTTCCTTCCAGTGTTTGAGGCCACCATCAAC
  CCCCAAGCTCACCCAGACCTCAGTGTCTTCCTCAAGCACATCACTGGCTTTGACAGTGTGGATGATGAAT
  CCAAACACAGTGGTCACATGTTTTCCTCCAAGAGTCCCAAACCCGAGGAGTGGACAATGGAAAACAACCC
  GTCTTATACTTACTATGCCTACTACATGTATGCAAACATCACGGTGCTCAACAGCCTGAGAAAGGAACGA
  GGCATGAATACATTTCTGTTTCGTCCCCACTGTGGGGAGGCTGGGGCTCTCACTCACCTCATGACAGCCT
  TCATGATAGCAGACAATATTTCTCATGGACTGAATTTAAAGAAGAGTCCTGTGCTACAGTACTTGTTTTT
  CCTAGCCCAGATTCCCATCGCCATGTCACCGCTGAGTAACAACAGTCTGTTCCTCGAATATGCAAAGAAT
  CCTTTCTTAGATTTCCTCCAGAAAGGCCTGATGATCTCACTGTCGACCGATGACCCGATGCAGTTCCACT
  TCACCAAGGAGCCCCTGATGGAAGAATACGCCATCGCTGCGCAAGTCTTCAAGCTGAGCACGTGTGACAT
  GTGTGAGGTGGCAAGGAACAGTGTCCTGCAGTGTGGGATTTCTCATGAGGAAAAAGCAAAGTTTCTGGGC
  AACAATTACCTTGAGGAAGGCCCTGTTGGAAATGACATCCGGAGGACAAATGTCGCTCAGATTCGCATGG
  CCTATCGTTATGAAACTTGGTGTTATGAACTCAATTTAATTGCTGAGGGTCTTAAAGCAACAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220907 representing NM_001033303
  Red=Cloning site Green=Tags(s)
  
  MPLFKLTGQGKQIDDAMRSFAEKVFASEVKDEGGRHEISPFDVDEICPISLHEMQAHIFHMENLSMDGRR
  KRRFQGRKTVNLSIPQSETSSTKLSHIEEFISSSPTYESVPDFQRVQITGDYASGVTVEDFEVVCKGLYR
  ALCIREKYMQKSFQRFPKTPSKYLRNIDGEALVGNESFYPVFTPPPKKGEDPFRTEDLPANLGYHLKMKA
  GVIYIYPDEAAANRDEPKPYPYPNLDDFLDDMNFLLALIAQGPVKTYAHRRLKFLSSKFQVHQMLNEMDE
  LKELKNNPHRDFYNCRKVDTHIHAAACMNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHP
  YDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHA
  EPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATIN
  PQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKER
  GMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKN
  PFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLG
  NNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033303
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033303.2, NP_001028475.2
• RefSeq Size: 2342 bp
• RefSeq ORF: 2238 bp
• Locus ID: 229665
• UniProt ID: Q3V1D3
• Cytogenetics: 3 45.25 cM
• MW: 86.6 kDa
• Gene Summary: AMP deaminase plays a critical role in energy metabolism.[UniProtKB/Swiss-Prot Function]