Smc1b (NM_080470) Mouse Tagged ORF Clone

CAT#: MR220884

Smc1b (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1B (Smc1b)

Product Images

Circular map for MR220884

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smc1b
• Synonyms: SMC-1B; SMC1beta; Smc1l2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220884 representing NM_080470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCACCTTGAGCTGCTGCTCGTGGAGAATTTCAAGTCGTGGCGAGGCCGCCAGGTCATCGGCCCTT
  TCAAGAGGTTCACCTGCATCATTGGCCCCAACGGCTCCGGAAAATCTAATGTAATGGATGCACTTAGTTT
  TGTAATGGGAGAAAAGACAACTAATTTAAGAGTAAAAAACATTCAAGAACTTATTCATGGAGCACATACT
  GGAAAACCTGTTTCTTCTTCTGCAAGTGTGACAATTATATACATAGAGGACAGTGGAGAAGAGAAAACAT
  TCACAAGGATTATCCGAGGGGGGTGCTCAGAATATCATTTTGGGGATAAACCCGTGAGTCGTTCTGTGTA
  TGTAGCCCAGTTGGAAAACATAGGCATAATAGTCAAAGCACAGAACTGTCTAGTTTTTCAGGGAACTGTA
  GAGTCAATATCCATGAAAAAGCCCAAAGAGAGAACCCAGTTTTTTGAAGAAATCAGTACTTCAGGAGAAT
  TTATAGGAGAATATGAGGCAAAGAAAAAGAAATTACAAAAAGCTGAAGAAGATGCACAATTTCATTTTAA
  TGTGAAAAAAAATGTAGCTGCAGAACGGAAGCATGCAAAAATAGAGAAAGAAGAGGCAGAACATTACCAA
  AATCTCCTTGAAGAATTGAAAATAAACAAGATACAACTGATGCTTTTCCAACTATATTATAATGAGGAAA
  AAATCAATGTTTTAAACACTGAATTAGAGCAAATGGACGGGAATTTAAGTGTTGTAAAAGACACTCTTTC
  TCACCATGAAAACATATTTAAAGCTAAGAAAAAGGATTATGGAATGTTAACTAGACAACTACAGCAAACA
  GCAAAAGAACTGAAATCTGTTGAAGCAATTTTAAATCAGAAGAGGCCTCAGTACATTAAGGCTAAAGAAA
  ACACTTCTCATCATCTAAAAAAATTAGATTTGTCTAAGAAATTGATAACAGACAATGAAAAACAATGTTC
  TAAGCAGGAAGATGGCATACGAGCCTTAGTGGCAGAACTGGCTGATTTGGATAGAGCATGGAAAAGTTTT
  GAAAAGCAGATGGAAGAGAAAATCTTACAAAAAGGGCGAGATATTGAATTGGAAAATAGCCAGCTGGATC
  GTTACAAACTGCTTAAGGAGCAAGTCAGACGGAAGGTTGGTATAATGACACAACAACTGGAAAAACTGCA
  GTGGGAACAGAAGGCAGAAAAAGAAAGACTTGCATTTGAAAAGAGGAGGCATGGAGACACTCAGGGAAAT
  CTAAAACAGATAAAAGAACAAATAGAAGAGCATAAAAAACGAATAGAGAAGTTGGAGGAATATACAAAGA
  CGTGCATGGATTGCTTGGAAGATAAAAAACAGCAAGAAGAGGCCCTGAAAAAAGAAATTGAAAATACGAA
  ATCAAGAATGTCTGAAGTTAATGAAGAATTGAGTCTTATTAGAAATGAATTGCAGAATGCTGGAATTGAT
  AACCATGAGGGAAAACGTCAGCAGAAAAGAGCAGAAGTTCTGGAACACCTTAAAAGACTTTACCCAGATT
  CTGTGTTTGGAAGACTGCTTGATCTGTGTCATCCTATTCATAAGAAGTACCAGCTGGCTGTGACTAAGCT
  TTTTGGCCGGTACATGGTTGCCATTGTTGTAGCCTCAGAAAAGATAGCGAAAGATTGTATTCGATTTCTG
  AAGGCAGAAAGAGCTGAACCTGAGACATTCCTTGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAC
  GACTAAGGGAAATTAAAGGCTGTAAGATGATGATCGATGTTATAAAGACCCAGTTTCCTCAGCTGAAGAA
  AGTGATTCAGTTTGTTTGTGGAAATGGCCTTGTCTGTGAGACTGTGGAAGAAGCAAGACATATTGCATTC
  GGTGGACCTGAAAGACGGAAGGCAGTAGCACTTGATGGAACACTGTTTTTGAAATCTGGAGTGATTTCTG
  GAGGGTCAAGTGACTTAAAGCACAAAGCTCTGTGCTGGGATGAGAAAGAGTTACACAATCTAAGAGACAA
  AAGAAGCCAACTAGTCCAAGAGCTAAAGGAGTTAATGAAGACACTCCGCAAGGAAACAGATCTGAAGCAA
  ATACAGACTTTAGTACAAGGAACCAATACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAAA
  AGAAGCACCTTGCTACATTTTACCGGGAACAATCTCAGCTACAAAGTGAATTACTGAATATTGATTCTCA
  ATGTACTATGTTGAGTGAAGGAATCAACAAACAGCAACAAAAAATTGAAGAATTTCAAGATAAGATAGAT
  GAGGTAGAAGATGACATATTCCAAGACTTCTGTGAAGAAATTGGTGTGGAAAATATCCGTGAATTTGAGA
  ATAAACATGTTAAACAGCAGCAAGAAAATGATCAAAAAAGATTAGAGTTTGAAAAACAAAAAACTCGGCT
  TAATATTCAGCTCGAATATAGTCGAAATCAGCTTAAGAAGAAACTGAATAATATCGACACATTAAAAACC
  ACAATCCAGAAGGGCAAAGAAGATATTGATAACCTAAAAAAGACCGAAGAAGAATGTCTGAAAATTGTTG
  AGGAACTCATGGTGAAGCAAGAGCAAATTAAGGAAGTGCTTGCCACACAGAGTTCCAACATTGAAAAAAT
  TCACATACAAATTGAAGAGGAACGCAAGAAGGTTTTGGCTGTTGATAGGGAAGTTGGAAAATTACAGAAG
  GAAGTTGTAATCATTCAGGGTTCTTTGGAACAGAAACTGCTAGAGAAACATAACTTGCTGCTAGATTGCA
  AAGTTCAAGACATTGACATAAGTCTTGTGCTGGGGTCATTGGAGGACATCATTGAAATGGAGCTAACTGA
  AACAGAAAGCACCCAGGCAACAGCTGATATCTATGAGAAAGAAGCATCCATCCAAATAGACTACAGCCCT
  CTAAGGGAGGATTTAAAGGCTCTACAATCAGATAAGGAGGTGGAGGCCCACCTTACACTCCTTCTACAGC
  AAGTAGCATCCCAAGAAAACACTCTACTGAAGACTACAGCTCCAAACTTGCGAGCACAGGAGAACTTAAA
  GACTGTCAGAGACAAGTTTCAAGAATCTGCAGATGTTTTTGAGGCCAGCAGAAAGGAAGCCAGAATATGT
  AGGCAAGAGTTTGAACAGGTGAAAAGACGGAGGTACGATGCTTTCAGTCAATGTTTTGAACACATCTCAG
  TCTCAATTGATCAAATCTACAAGAAGCTCTGCAGGAACAACAGTGCCCAGGCATTTCTTAGCCCAGAGAA
  CCCTGAAGAACCTTACTTAGATGGAATTAGCTACAACTGTGTGGCTCCAGGCAAACGGTTCATGCCCATG
  GACAACCTGTCAGGGGGAGAAAAGTGTGTGGCTGCTCTGGCTCTTCTGTTTGCTGTACACAGTTTTCGGC
  CTGCTCCATTCTTTGTATTAGATGAAGTAGATGCAGCCCTGGACAATACTAACATTGGCAAAGTCTCAAG
  TTACATCAAAGAGCAGAGTCAGGAACAGTTTCAGATGATAATCATTTCCCTGAAAGAGGAGTTCTACTCC
  AAAGCTGATGCACTGATAGGCGTCTACCCAGAGCACAATGAGTGCATGTTCAGCCATGTGTTGACTCTGG
  ACCTTTCCAAGTATCCAGACACCGAAGACCAAGAAGGCAGCAGGAGCCACCGGAAGCCCAGAGTACCACG
  AGTATCAATGTCTCCAAAGTCTCCCCAGTCTCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220884 representing NM_080470
  Red=Cloning site Green=Tags(s)
  
  MGHLELLLVENFKSWRGRQVIGPFKRFTCIIGPNGSGKSNVMDALSFVMGEKTTNLRVKNIQELIHGAHT
  GKPVSSSASVTIIYIEDSGEEKTFTRIIRGGCSEYHFGDKPVSRSVYVAQLENIGIIVKAQNCLVFQGTV
  ESISMKKPKERTQFFEEISTSGEFIGEYEAKKKKLQKAEEDAQFHFNVKKNVAAERKHAKIEKEEAEHYQ
  NLLEELKINKIQLMLFQLYYNEEKINVLNTELEQMDGNLSVVKDTLSHHENIFKAKKKDYGMLTRQLQQT
  AKELKSVEAILNQKRPQYIKAKENTSHHLKKLDLSKKLITDNEKQCSKQEDGIRALVAELADLDRAWKSF
  EKQMEEKILQKGRDIELENSQLDRYKLLKEQVRRKVGIMTQQLEKLQWEQKAEKERLAFEKRRHGDTQGN
  LKQIKEQIEEHKKRIEKLEEYTKTCMDCLEDKKQQEEALKKEIENTKSRMSEVNEELSLIRNELQNAGID
  NHEGKRQQKRAEVLEHLKRLYPDSVFGRLLDLCHPIHKKYQLAVTKLFGRYMVAIVVASEKIAKDCIRFL
  KAERAEPETFLALDYLDIKPINERLREIKGCKMMIDVIKTQFPQLKKVIQFVCGNGLVCETVEEARHIAF
  GGPERRKAVALDGTLFLKSGVISGGSSDLKHKALCWDEKELHNLRDKRSQLVQELKELMKTLRKETDLKQ
  IQTLVQGTNTRLKYSQNELEMIKKKHLATFYREQSQLQSELLNIDSQCTMLSEGINKQQQKIEEFQDKID
  EVEDDIFQDFCEEIGVENIREFENKHVKQQQENDQKRLEFEKQKTRLNIQLEYSRNQLKKKLNNIDTLKT
  TIQKGKEDIDNLKKTEEECLKIVEELMVKQEQIKEVLATQSSNIEKIHIQIEEERKKVLAVDREVGKLQK
  EVVIIQGSLEQKLLEKHNLLLDCKVQDIDISLVLGSLEDIIEMELTETESTQATADIYEKEASIQIDYSP
  LREDLKALQSDKEVEAHLTLLLQQVASQENTLLKTTAPNLRAQENLKTVRDKFQESADVFEASRKEARIC
  RQEFEQVKRRRYDAFSQCFEHISVSIDQIYKKLCRNNSAQAFLSPENPEEPYLDGISYNCVAPGKRFMPM
  DNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSSYIKEQSQEQFQMIIISLKEEFYS
  KADALIGVYPEHNECMFSHVLTLDLSKYPDTEDQEGSRSHRKPRVPRVSMSPKSPQSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080470
• ORF Size: 3744 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080470.1, NP_536718.1
• RefSeq Size: 4056 bp
• RefSeq ORF: 3747 bp
• Locus ID: 140557
• UniProt ID: Q920F6
• Cytogenetics: 15 E2
• MW: 145 kDa
• Gene Summary: Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.[UniProtKB/Swiss-Prot Function]