Hormad2 (NM_029458) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220735
Hormad2 (Myc-DDK-tagged) - Mouse HORMA domain containing 2 (Hormad2)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hormad2
Synonyms 4930529M09Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220735 representing NM_029458
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACTGCGCAGCTCTCTCACAACACCAGAACACTGAAGGCGTCTAAGAACACGATTTTCCCGTCCC
AAGTGACAAATGAGCATGAGTCACTGGTGGTGGTGAAGAAGCTTTTTGCTACTTGTATCTCATGTATAAC
GTACCTGAGGGGCTTGTTTCCAGAGAGCTCCTACAGAGACCGCCGTTTGGATGACCTCAGTTTAAAAATT
CTCCGGGAAGACAAAAAGTGTCCGGGGTCATTACATATTATTAAATGGATTCAAGGTTGTTTTGATGCTT
TGGAAAAGAGATACCTACACATGGCAGTATTAACACTTTACACAAATCCCAAGGAACCTGAGAAAGTGAC
TGAGATATACCAATTCAGATTCAAATACACAAAAAAAGGAACCACTATGGACTTTGACAGCAGCAGCACA
AGCTTTGAAAGTGGGACAGACAGTGAAGATATTAAGAAAGCCTGTTCCCTACTAATCCGTCAATTGTATA
TACTGATGCAGAATCTTGGACCCCTTCCGAATGATGTTATACTTACTATGAAACTCCATTATTATAACTC
AGTGACCCCACATGATTACCAACCCCCTGGCTTTAAAGAAGCGGTGAATTCACACTTCCTGCTGTTTGAG
GGGGAGCCTGTGAGCCTTCGAATGGGTTCAGTCTCCTCTGGCTTTCACAGCATGAAAGTAAAAGTCACAA
CGGAGGCCACCAGAATGCTCGATGGGGAGAACAGTGTCAGTCAGGACGATGGCACTACTGAGATAGCTCA
TCAGGGGCTAGACTGTGACGAGGAAGAGGAGGCCTGCGGCAGTCAAGTCCAAAGAATGAATTTTGTGCAC
ATTGAGCCAAGTTTTGAAAGTTCCAGAAAGAAGAAGAAGGTCAGCGAACCAGTGACAGTCTTCATTCCTA
ACAGAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220735 representing NM_029458
Red=Cloning site Green=Tags(s)

MATAQLSHNTRTLKASKNTIFPSQVTNEHESLVVVKKLFATCISCITYLRGLFPESSYRDRRLDDLSLKI
LREDKKCPGSLHIIKWIQGCFDALEKRYLHMAVLTLYTNPKEPEKVTEIYQFRFKYTKKGTTMDFDSSST
SFESGTDSEDIKKACSLLIRQLYILMQNLGPLPNDVILTMKLHYYNSVTPHDYQPPGFKEAVNSHFLLFE
GEPVSLRMGSVSSGFHSMKVKVTTEATRMLDGENSVSQDDGTTEIAHQGLDCDEEEEACGSQVQRMNFVH
IEPSFESSRKKKKVSEPVTVFIPNRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029458
ORF Size 918 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029458.1, NP_083734.1
RefSeq Size 1343 bp
RefSeq ORF 921 bp
Locus ID 75828
UniProt ID Q5SQP1
Cytogenetics 11 A1
MW 34.8 kDa
Summary Essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes. Required for the DNA double-strand break-independent, BRCA1-dependent activation of ATR on the sex chromosomes that is essential for normal sex body formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029458" in other vectors (4)
SKU Description Size Price
MC211699 Hormad2 (untagged) - Mouse HORMA domain containing 2 (Hormad2), (10ug) 10 ug
$330.00
MG220735 Hormad2 (tGFP-tagged) - Mouse HORMA domain containing 2 (Hormad2), (10ug) 10 ug
$650.00
MR220735L3 Lenti ORF clone of Hormad2 (Myc-DDK-tagged) - Mouse HORMA domain containing 2 (Hormad2) 10 ug
$750.00
MR220735L4 Lenti ORF clone of Hormad2 (mGFP-tagged) - Mouse HORMA domain containing 2 (Hormad2) 10 ug
$750.00

Plasmid Map for MR220735

Download sequence for

Cloning scheme for MR220735

Circular map for MR220735

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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