Rundc3a (NM_016759) Mouse Tagged ORF Clone

CAT#: MR220710

Rundc3a (Myc-DDK-tagged) - Mouse RUN domain containing 3A (Rundc3a)

Product Images

Circular map for MR220710

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rundc3a
• Synonyms: Rap2ip; RPIP-8; Rpip8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220710 representing NM_016759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCGAGCTTTGTCCAGACCACCATGGCTCTGGGGCTGCCCTCCAAGAAAGCATCTTCCCGCAACG
  TGATCGTGGAGCGCAGGAACCTGATCACCGTGTGCAGGTTCTCTGTGAAAACCCTGCTAGAGAAGTACAC
  AGCAGAACCCATTGATGACTCTTCCGAGGAGTTTGTTAATTTCGCAGCCATTTTAGAGCAGATCCTCAGC
  CACCGATTTAAAGCTTGTGCCCCAGCAGGGCCAGCGAGCTGGTTCAGCTCAGATGGACAACGGGGCTTCT
  GGGACTACATCCGGCTGGCCTGCAGCAAAGTGCCCAACAACTGTGTGAGCAGCATTGAGAACATGGAGAA
  CATCAGCACAGCTAGAGCCAAGGGCCGGGCGTGGATCCGGGTGGCCCTGATGGAGAAGCGTATGTCGGAA
  TACATCACCACAGCTCTTCGGGACAACCGAACTACCAGACGGTTCTATGACTCCGGAGCCATCATGCTGC
  GGGAGGAAGCCACTGTCCTCACTGGGATGCTGATCGGGCTCAGCGCCATCGACTTCAGCTTCTGTCTAAA
  GGGCGAAGTTCTGGATGGGAAGACACCGGTGGTCATCGATTACACACCCTACCTAAAATTCACCCAAAGC
  TACGACTACCTGACGGATGAGGAGGAGAGGCACAGTGCCGAGAGCAGCACCAGCGAGGACAACTCACCAG
  AGCACCCCTACCTGCCTCTCGTCACCGATGAAGACAGTTGGTACAACAAGTGGCACAAGATGGAACAGAA
  GTTTCGCATTGTCTACGCACAGAAGGGATACCTGGAGGAGCTGGTGCGGCTGCGCGAGTCGCAGCTGAAG
  GACTTGGAGGCGGAGAACCGGCGGCTGCAGCTGCAGCTGGAGGAGGCCGCGGCACAAAATCAGCGTGAGA
  AGCGGGAGCTGGAAGGCGTGATCCTGGAGCTGCAGGAGCAGCTGACAGGTCTGATCCCCGGTGACCATGC
  CCCCCTGGCCCAGGGTTCCAAGGAGCTCACCACATCCCTAGTCAACCAGTGGCCCTCCCTGAGCACACTT
  CATAGGCCCGAGGGTGCCAGCAACTCCAAGCTATATCGGAGACACAGCTTCATGAGCACGGAGCCGCTGT
  CTGCAGAGGCCAGCCTGAGCTCAGACTCCCAGCGCCTGGGAGAGGCCAAGAGGGACGAGGAACCCTGGGG
  CCCCATCGGGAAGGACCCCACGCCCTCCATGCTGGGCCTCTGCGGCTCCCTGGCCTCCATCCCCAGCTGC
  AAGTCCCTGGCAAGCTTCAAATCCAACGAATGCCTGGTGAGCGACAGCCCTGAGGGCAGCCCGGCGCTCA
  GCCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220710 representing NM_016759
  Red=Cloning site Green=Tags(s)
  
  MEASFVQTTMALGLPSKKASSRNVIVERRNLITVCRFSVKTLLEKYTAEPIDDSSEEFVNFAAILEQILS
  HRFKACAPAGPASWFSSDGQRGFWDYIRLACSKVPNNCVSSIENMENISTARAKGRAWIRVALMEKRMSE
  YITTALRDNRTTRRFYDSGAIMLREEATVLTGMLIGLSAIDFSFCLKGEVLDGKTPVVIDYTPYLKFTQS
  YDYLTDEEERHSAESSTSEDNSPEHPYLPLVTDEDSWYNKWHKMEQKFRIVYAQKGYLEELVRLRESQLK
  DLEAENRRLQLQLEEAAAQNQREKRELEGVILELQEQLTGLIPGDHAPLAQGSKELTTSLVNQWPSLSTL
  HRPEGASNSKLYRRHSFMSTEPLSAEASLSSDSQRLGEAKRDEEPWGPIGKDPTPSMLGLCGSLASIPSC
  KSLASFKSNECLVSDSPEGSPALSPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016759
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016759.5
• RefSeq Size: 2043 bp
• RefSeq ORF: 1341 bp
• Locus ID: 51799
• UniProt ID: O08576
• Cytogenetics: 11 D
• MW: 50.5 kDa
• Gene Summary: May act as an effector of RAP2A in neuronal cells.[UniProtKB/Swiss-Prot Function]