Ttc19 (NM_029704) Mouse Tagged ORF Clone

CAT#: MR220506

Ttc19 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 19 (Ttc19), transcript variant 2

Product Images

Circular map for MR220506

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ttc19
• Synonyms: 2010204O13Rik; 2810460C24Rik; AI505442
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220506 representing NM_029704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGTTGCTTCGCTGGCGTCTGGGTCGAACCCTGCTGCGGGCCGCGGGCCGGCGGTGTGGGGGTT
  GTACGGCGCGCCTGCTGCCGGAGCGGACGGGCGACGCGGGGACGGGCGCGGAGCGGCTGCGGACCCGAGG
  CGCGCCGGCGCGGGGGCACGGGGTGCTGCCGCTGTTGGCAGCTCTCGCCTGGTTCTCGCGACCCGCGGCG
  ACGGCCGAGCAGCCGGGAGAGGACGCGTCAGACGAGGCGGAGGCCGAGATCATCCAGCTGCTGAAGCAAG
  CCAAGTTGAGCATCATGAAAGACGAGCCAGAAGCGGCCGAGCTGATTTTGCATGACGCTCTTCGCCTTGC
  GTATGAGAGTGACAACAGGAAGGCCATCACGTACACTTATGACCTGGCAGAACAGCTTTTTAAAGCAACG
  ATGAGTTATCTGCTTGGAGGAGGCATGAAGCAGGAAGACAATGCAATAATTGAAATCTCTCTGAAACTGG
  CCAACATCTATGCTGCTCAGAATAAACAGGAATTTGCCCTTGCTGGCTATGAATTCTGCATTTCAACTCT
  AGAGGGGAAAATTGAAAGAGAAAAGGAATTAGCAGAAGATATTATGTCAGAAGAAACAGCCAATACTTAC
  CTCCTCCTTGGTATGTGCTTAGACTCTTGTGCACGCTATCTGCTATTCTCCAAGCAGCTATCACAGGCAC
  AAAGGATGTATGAAAAAGCCCTGCAGATTTGTCAAGAAATACAAGGAGAAAGACACCCACAGACCATTGT
  GCTGATGAGTGACTTGGCTACTACTCTGGATGCACAGGGCCACTTTGATGATGCCTACATTTATATGCAG
  AGGGCGTCAGATCTGGCAAGAGAGATAAATCACCCTGAGCTGCACATGGTACTCAGCAATCTGGCTGCCA
  TTTTGATACACAGAGAGCGATACACACAAGCAAAGGAGATCTACCAGGAAGCGCTGAAGCGAGCAGAGCT
  GAAAAGAGATGAGGTTTCTGTTCAGCACATCAGGGAAGAATTGGCTGAACTGTCAAGAAAAAGTAGACGT
  TTGACT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220506 representing NM_029704
  Red=Cloning site Green=Tags(s)
  
  MFRLLRWRLGRTLLRAAGRRCGGCTARLLPERTGDAGTGAERLRTRGAPARGHGVLPLLAALAWFSRPAA
  TAEQPGEDASDEAEAEIIQLLKQAKLSIMKDEPEAAELILHDALRLAYESDNRKAITYTYDLAEQLFKAT
  MSYLLGGGMKQEDNAIIEISLKLANIYAAQNKQEFALAGYEFCISTLEGKIEREKELAEDIMSEETANTY
  LLLGMCLDSCARYLLFSKQLSQAQRMYEKALQICQEIQGERHPQTIVLMSDLATTLDAQGHFDDAYIYMQ
  RASDLAREINHPELHMVLSNLAAILIHRERYTQAKEIYQEALKRAELKRDEVSVQHIREELAELSRKSRR
  LT
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029704
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029704.2, NP_083980.2
• RefSeq Size: 3397 bp
• RefSeq ORF: 1059 bp
• Locus ID: 72795
• UniProt ID: Q8CC21
• Cytogenetics: 11 B2
• MW: 40.2 kDa
• Gene Summary: Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed:21278747, PubMed:28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation into the complex III and whose presence is detrimental for its catalytic activity (PubMed:28673544).[UniProtKB/Swiss-Prot Function]