Nt5dc1 (NM_176968) Mouse Tagged ORF Clone

CAT#: MR220371

  • TrueORF®

Nt5dc1 (Myc-DDK-tagged) - Mouse 5'-nucleotidase domain containing 1 (Nt5dc1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_176968" in other vectors (4)


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Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Nt5dc1
Synonyms 6030401B09Rik; AW987726; Nt5c2l1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR220371 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGCACTTCTCCCTGGGCGCCTGCGACGTGGTCGGCTTCGATCTCGACCACACGCTGTGCCGCT
ACAACCCGCCCGAGAGCGCCCGGCTCATTTATAACAGCTTTGCTCAGTTCTTAGTCAAGGAGAAAGGATA
TGATGAGGGGCTGCTCACTCTGACTCCAGAGGACTGGGATTTCTGTTGCAAGGGTTTGGCATTGGATCTG
GAAGATGGGACCTTCATTAAACTTGCAGCTGATGGCACTGTGCTCAGGGCAAGCCACGGCACCAAGATGA
TGACTCCAGAGGCACTGACTGAAGCCTTTGGCAAGAAGGAGTGGAGGCACTGTGTGTCAGACAAACGCTG
TACATCAGACAAGCCCGGTGTGTCCGACATTCCTTGCTGCTCAGGAAAGTGTTATTTCTATGATAACTAC
TTTGACCTGCCGGGGGCTCTTCTGTGTGCCAGAGTGGTGGACTCTTTAACAAAGAACAGAGGTCAAAAAA
CATTTGATTTTTGGAAGGATGTAGTTGCTGGTATACAACACAATTTTAAAATGTCAGCCTTTAAGGAAAA
TTGTGGATTCTTCTTTCCGGAAATAAACAGAAATCTAGGCAAATATGTGCACCGCTGTCCTGAGTCTGTA
AGAAAGTGGCTTCGACAGCTGAAGGACGCTGGGAAAATTACTATGTTGATAACTAGTTCTCACAGTGATT
ACTGTAAACTTCTTGGCAGTTACATCCTTGGGGAGGATTTTGCAGACCTTTTTGACATTGTGATTACAAA
CGCATTAAAGCCTGGATTCTTCCCACATTTCCCAAGCCAGAGGCCTTTCTACACACTCGAGAATGATGAA
GAGAAGGATGAGCTGCCGTCTCTGGATAAACCGGGCTGGTACTCCCAAGGGAACGCTGCCCACCTTTATG
AGCTTCTGAAGAAAATGACCAGCAAGCCTGAACCCAAGGTCGTTTACTTTGGTGACAGCATGCATTCAGA
CATTTTCCCAGCCCATCACTATACTAACTGGGAGACTGTCCTTATTCTCGAGGAGCTTCAGGGGCCAGAA
ATGGAAAAGCCTGAGGAAGCAGAGCCTCTGGAGAAAAGAGGAAAATATGAGGCACCAATGGTAAAGCCTC
TAAACACCTTGTCTAATAAGTGGGGCTCATACTTTATTGACTCAGTCTCCGGACGGGGGAGGGCAGAAGA
CTCTGTGGTTTATACATGGTCCTCTAAGAGAATCAGCACCTACAGTACGATTGCAATTCCAAACATTGAA
TCTATTGCAGAGTTACCCCTGGACTACAAATTTACAAGATTTTCTACAAACAATTCAAAAATGGCTGGTT
ACTATCCCTTGGTCCATCATACCTTGGGTTCTCAAGACACAGACAGCAAAATAATATCTACTGAAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR220371 protein sequence
Red=Cloning site Green=Tags(s)

MAQHFSLGACDVVGFDLDHTLCRYNPPESARLIYNSFAQFLVKEKGYDEGLLTLTPEDWDFCCKGLALDL
EDGTFIKLAADGTVLRASHGTKMMTPEALTEAFGKKEWRHCVSDKRCTSDKPGVSDIPCCSGKCYFYDNY
FDLPGALLCARVVDSLTKNRGQKTFDFWKDVVAGIQHNFKMSAFKENCGFFFPEINRNLGKYVHRCPESV
RKWLRQLKDAGKITMLITSSHSDYCKLLGSYILGEDFADLFDIVITNALKPGFFPHFPSQRPFYTLENDE
EKDELPSLDKPGWYSQGNAAHLYELLKKMTSKPEPKVVYFGDSMHSDIFPAHHYTNWETVLILEELQGPE
MEKPEEAEPLEKRGKYEAPMVKPLNTLSNKWGSYFIDSVSGRGRAEDSVVYTWSSKRISTYSTIAIPNIE
SIAELPLDYKFTRFSTNNSKMAGYYPLVHHTLGSQDTDSKIISTEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_176968
ORF Size 1401 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176968.1, NM_176968.2, NM_176968.3, NM_176968.4, NM_176968.5, NP_795942.2
RefSeq Size 2716 bp
RefSeq ORF 1404 bp
Locus ID 319638
UniProt ID Q8C5P5
Cytogenetics 10 B1
MW 52.9 kDa

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