Alyref (NM_011568) Mouse Tagged ORF Clone

CAT#: MR220236

Alyref (Myc-DDK-tagged) - Mouse THO complex 4 (Thoc4)

Product Images

Circular map for MR220236

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alyref
• Synonyms: ALY; Aly; REF1; Ref1; Ref1-I; Refbp1; Tho4; Thoc4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220236 representing NM_011568
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAAAATGGACATGTCTTTGGACGACATCATTAAGCTGAACCGGAGCCAGCGAGGAGGCCGCG
  GCGGGGGCCGGGGTCGCGGCAGGGCCGGCTCCCAGGGCGGCCGCGGCGGCGCAGTGCAGGCCGCCGCGCG
  GGTGAATCGAGGCGGCGGGCCTATGAGGAACCGGCCCGCCATCGCCCGCGGCGCCGCAGGCGGCGGCAGG
  AACCGGCCGGCGCCGTACAGCAGACCGAAACAACTTCCCGACAAATGGCAGCACGACCTCTTCGACAGCG
  GCTTCGGGGGTGGAGCCGGCGTGGAGACGGGCGGGAAGCTGCTGGTGTCCAACCTGGACTTCGGAGTGTC
  AGATGCTGATATTCAGGAACTCTTTGCTGAATTTGGGACATTGAAAAAAGCTGCTGTGCACTATGATCGC
  TCTGGACGAAGTTTAGGGACAGCAGATGTGCACTTTGAACGGAAAGCAGATGCCCTGAAGGCTATGAAAC
  AGTACAATGGTGTCCCTTTGGATGGCCGCCCTATGAACATCCAGCTTGTCACATCACAGATTGATACACA
  GCGAAGACCTGCACAGAGCATAAACAGAGGCGGCATGACAAGAAACCGTGGCTCTGGAGGTTTTGGTGGT
  GGTGGCACCAGGAGAGGGACACGTGGAGGCAGCCGGGGAAGAGGTAGAGGCACCGGCAGGAACTCAAAGC
  AGCAGCTTTCTGCAGAGGAGTTGGACGCACAGCTGGATGCTTACAATGCAAGGATGGACACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220236 representing NM_011568
  Red=Cloning site Green=Tags(s)
  
  MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGAVQAAARVNRGGGPMRNRPAIARGAAGGGR
  NRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDR
  SGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDTQRRPAQSINRGGMTRNRGSGGFGG
  GGTRRGTRGGSRGRGRGTGRNSKQQLSAEELDAQLDAYNARMDTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011568
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011568.1, NP_035698.1
• RefSeq Size: 1132 bp
• RefSeq ORF: 768 bp
• Locus ID: 21681
• UniProt ID: O08583
• Cytogenetics: 11 E2
• MW: 26.9 kDa
• Gene Summary: Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.[UniProtKB/Swiss-Prot Function]