Cd46 (NM_010778) Mouse Tagged ORF Clone

CAT#: MR220201

  • TrueORF®

Cd46 (Myc-DDK-tagged) - Mouse CD46 antigen, complement regulatory protein (Cd46)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_010778" in other vectors (4)


Interest in protein/lysate? Submit request here!

Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Cd46
Synonyms Mcp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR220201 representing NM_010778
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGCGGCGCCTCTTATGCCAGACTCAACGCACCCCTGTCGCAGAAGGAAGAGCTACACTTTCTTCT
GGTGCTCCTTGGGCGTCTATGCTGAGGCCCTTCTGTTTCTGCTGTCTCATTTATCTGATGCCTGTGAACT
ACCACGGCCATTTGAAGCTATGGAACTCAAGGGTACACCTAAACTCTTTTATGCCGTTGGAGAGAAAATA
GAATATAAGTGTAAAAAAGGATACCTGTATCTGTCTCCATACCTGATGATTGCTACCTGTGAGCCAAATC
ATACATGGGTCCCTATTTCAGATGCTGGTTGTATTAAAGTTCAATGTACTATGTTACAAGACCCTTCATT
TGGCAAAGTATACTACATAGATGGCAGCTTTTCATGGGGTGCTCGAGCTAAATTTACTTGTATGGAAGGT
TATTACGTAGTTGGTATGTCAGTTCTACACTGTGTGCTTAAAGGTGATGATGAAGCATACTGGAATGGCT
ATCCCCCACATTGTGAAAAGATTTATTGTTTACCACCTCCAAAAATAAAAAATGGAACACATACCCTTAC
TGATATAAATGTATTCAAATACCATGAAGCAGTAAGTTACAGTTGTGATCCTACCCCAGGGCCAGATAAG
TTTTCCCTTGTTGGAACAAGCATGATATTCTGTGCTGGCCATAACACCTGGAGTAACAGCCCTCCGGAGT
GTAAAGTGGTAAAATGTCCAAATCCAGTACTACAAAATGGAAGACTGATATCAGGAGCTGGCGAAATATT
TTCCTATCAATCAACAGTGATGTTTGAGTGTTTGCAAGGATTTTACATGGAAGGCAGTAGCATGGTGATC
TGTAGTGCTAATAACTCTTGGGAGCCATCTATCCCAAAATGTCTTAAAGGTCCTAGGCCTACTCATCCTA
CCAAGCCTCCAGTTTATAATTATACAGGATATCCTAGTCCCCGTGAAGGAATATTTAGCCAAGAATTAGA
TGCATGGATTATTGCTTTGATTGTTATTACGTCAATTGTTGGAGTTTTTATACTTTGTCTCATTGTGCTC
AGGTGTTTTGAGCACAGGAAGAAAACAAATGTATCTGCAGCAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR220201 representing NM_010778
Red=Cloning site Green=Tags(s)

MTAAPLMPDSTHPCRRRKSYTFFWCSLGVYAEALLFLLSHLSDACELPRPFEAMELKGTPKLFYAVGEKI
EYKCKKGYLYLSPYLMIATCEPNHTWVPISDAGCIKVQCTMLQDPSFGKVYYIDGSFSWGARAKFTCMEG
YYVVGMSVLHCVLKGDDEAYWNGYPPHCEKIYCLPPPKIKNGTHTLTDINVFKYHEAVSYSCDPTPGPDK
FSLVGTSMIFCAGHNTWSNSPPECKVVKCPNPVLQNGRLISGAGEIFSYQSTVMFECLQGFYMEGSSMVI
CSANNSWEPSIPKCLKGPRPTHPTKPPVYNYTGYPSPREGIFSQELDAWIIALIVITSIVGVFILCLIVL
RCFEHRKKTNVSAAR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010778
ORF Size 1095 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010778.4
RefSeq Size 1213 bp
RefSeq ORF 1098 bp
Locus ID 17221
UniProt ID O88174
Cytogenetics 1 98.41 cM
MW 41.3 kDa
Gene Summary May be involved in the fusion of the spermatozoa with the oocyte during fertilization.[UniProtKB/Swiss-Prot Function]

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