Sept6 (NM_001177324) Mouse Tagged ORF Clone

CAT#: MR220085

41523 (Myc-DDK-tagged) - Mouse septin 6 (Sept6), transcript variant 1

Product Images

Circular map for MR220085

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sept6
• Synonyms: 2810035H17Rik; C920001C06Rik; mKIAA0128; Sep6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220085 representing NM_001177324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCCGATATAGCTCGCCAGGTGGGCGAAGATTGTCGAACTGTCCCCCTGGCTGGACATGTGG
  GGTTCGACAGCTTGCCTGACCAGCTGGTGAATAAGTCAGTCAGCCAGGGCTTCTGCTTCAACATCCTGTG
  CGTGGGGGAGACAGGTTTGGGCAAGTCCACGCTCATGGATACCCTGTTTAACACCAAGTTTGAAGGGGAG
  CCAGCGACCCACACACAGCCAGGGGTTCAGCTCCAGTCCAATACCTACGACCTCCAGGAGAGCAACGTGG
  GGTTGAAACTCACAATTGTGAGCACTGTAGGCTTTGGGGATCAGATCAACAAGGAGGACAGCTACAAGCC
  CATCGTGGAATTCATTGATGCTCAGTTTGAGGCCTACCTACAAGAGGAGCTGAAGATCCGAAGAGTACTG
  CACTCCTACCATGACTCCCGAATCCATGTCTGCTTGTATTTCATTGCCCCGACAGGACATTCGCTTAAGT
  CTCTGGACCTAGTGACCATGAAGAAGCTGGATAGTAAGGTGAACATCATTCCCGTTATTGCCAAGTCAGA
  TGCCATTTCTAAGAGTGAGCTGGCAAAGTTCAAGATCAAAATCACCAGCGAACTTGTGAGCAATGGAGTC
  CAGATCTATCAGTTCCCCACAGACGATGAGTCGGTGAGCGAGATCAATGGAACCATGAATGCCCACCTGC
  CGTTTGCTGTTGTTGGCAGCACAGAAGAAGTGAAGATAGGCAACAAGATGATGCGGGCTCGGCAGTATCC
  TTGGGGGACTGTGCAGGTTGAAAACGAGGCTCACTGCGACTTTGTGAAGCTGCGGGAGATGCTGATTCGG
  GTCAACATGGAGGATCTGCGGGAGCAGACCCACGCCCGGCACTATGAATTGTACCGCCGCTGTAAGCTGG
  AGGAGATGGGCTTCAAGGACACCGACCCTGACAGCAAACCATTCAGTTTGCAGGAGACATATGAGGCAAA
  GAGAAATGAGTTCCTTGGGGAGCTCCAGAAGAAAGAAGAGGAGATGAGGCAGATGTTTGTCCAGAGAGTC
  AAAGAGAAAGAAGCGGAGCTCAAGGAGGCTGAAAAAGAGCTGCATGAGAAATTTGACCGTCTGAAGAAAC
  TGCACCAGGAAGAGAAGAAGAAGCTGGAGGATAAGAAGAAATGTCTGGATGAGGAAATGAACGCCTTCAA
  GCAGAGAAAGGCGGCAGCTGAGCTGCTCCAGTCTCAGGGCTCTCAGGCTGGGGGCTCACAGACTCTGAAA
  AGGGACAAAGAGAAGAAGAATAACCCATGGCTGTGTATTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220085 representing NM_001177324
  Red=Cloning site Green=Tags(s)
  
  MAAADIARQVGEDCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGE
  PATHTQPGVQLQSNTYDLQESNVGLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVL
  HSYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPVIAKSDAISKSELAKFKIKITSELVSNGV
  QIYQFPTDDESVSEINGTMNAHLPFAVVGSTEEVKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIR
  VNMEDLREQTHARHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRV
  KEKEAELKEAEKELHEKFDRLKKLHQEEKKKLEDKKKCLDEEMNAFKQRKAAAELLQSQGSQAGGSQTLK
  RDKEKKNNPWLCIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177324
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177324.1, NP_001170795.1
• RefSeq Size: 3494 bp
• RefSeq ORF: 1305 bp
• Locus ID: 56526
• UniProt ID: Q9R1T4
• Cytogenetics: X A3.3
• MW: 50.1 kDa
• Gene Summary: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]