Gbp2 (NM_010260) Mouse Tagged ORF Clone

CAT#: MR220050

Gbp2 (Myc-DDK-tagged) - Mouse guanylate binding protein 2 (Gbp2)

Product Images

Circular map for MR220050

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gbp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220050 representing NM_010260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGAGATCCACATGTCGGAACCCATGTGCCTCATTGAGAACACTGAGGCTCAACTAGTGATCA
  ACCAGGAGGCTCTGAGGATCCTGTCTGCCATTACACAGCCTGTGGTGGTGGTGGCAATCGTGGGCCTCTA
  CCGCACAGGCAAATCCTACCTGATGAACAAGCTAGCTGGGAAGAGGACAGGCTTTTCCCTGGGCTCCACT
  GTGCAGTCTCACACCAAGGGCATCTGGATGTGGTGTGTGCCTCACCCCAAGAAGGCAGGGCAAACCCTGG
  TTCTGCTTGACACTGAGGGCCTTGAAGATGTTGAGAAGGGTGACAACCAGAATGACTGCTGGATCTTTGC
  TTTGGCAGTCCTCCTCAGCAGCACCTTCATCTACAACAGCATAGGAACCATCAACCAGCAGGCCATGGAC
  CAGCTGCACTATGTGACGGAGCTAACTGATCTTATCAAGTCAAAGTCATCACCTGACCAGAGTGGGGTAG
  ACGATTCCGCTAACTTTGTGGGCTTCTTTCCAACCTTTGTGTGGACTCTGAGAGATTTCTCCCTCGAGCT
  GGAGGTTAACGGAAAACCCGTCACTTCTGATGAGTACCTGGAACATTCCCTGACCCTGAAAAAAGGAGCT
  GATAAGAAAACTAAAAGCTTTAATGAGCCGCGACTGTGCATCAGGAAATTCTTTCCAAAGAGGAAGTGCT
  TCATCTTTGACAGGCCTGCTCAGAGGAAGCAACTTAGCAAACTGGAGACTCTGCGGGAGGAGGAGCTGTG
  TGGTGAATTTGTAGAACAAGTTGCAGAATTCACCTCATACATCTTGAGCTACTCTTCTGTCAAGACTCTG
  TGTGGTGGCATCATAGTCAATGGGCCACGTCTAAAGAGCCTGGTGCAGACCTATGTTGGTGCCATCAGCA
  ATGGGTCTCTCCCCTGCATGGAGAGTGCTGTGCTGACTTTGGCCCAGATAGAGAACTCAGCAGCAGTGCA
  AAAGGCCATCACCCACTATGAAGAACAGATGAATCAGAAGATTCAGATGCCCACAGAAACCCTCCAGGAG
  CTCCTGGATCTGCACAGGCCAATTGAGAGTGAGGCCATTGAGGTCTTCCTGAAGAATTCTTTCAAGGATG
  TAGACCAAAAGTTCCAGACAGAATTAGGGAACCTGCTGGTAGCCAAGCGAGATGCCTTTATCAAGAAGAA
  CATGGATGTCTCATCAGCTCGTTGCTCAGACTTGCTGGAGGATATTTTTGGACCCCTGGAAGAAGAAGTA
  AAATTAGGGACATTTTCTAAACCAGGAGGTTACTACCTCTTCCTTCAAATGAGACAAGAGCTAGAGAAAA
  AGTATAACCAGGCTCCTGGGAAGGGGCTCCAGGCAGAAGCGATGCTGAAAAACTACTTTGATTCCAAGGC
  AGATGTTGTTGAAACACTTCTACAGACGGATCAGTCACTCACAGAGGCAGCAAAGGAGGTAGAAGAGGAA
  CGTACGAAGGCTGAAGCTGCTGAAGCTGCAAACAGAGAGTTAGAAAAGAAGCAGAAGGAGTTCGAGCTGA
  TGATGCAGCAGAAGGAAAAGAGTTACCAGGAGCATGTGAAGAAGCTGACTGAGAAGATGAAAGACGAACA
  GAAACAGTTATTAGCAGAACAGGAAAACATCATAGCTGCTAAACTTCGGGAACAGGAAAAATTTCTTAAG
  GAAGGATTCGAGAATGAGAGCAAAAAACTTATTCGAGAGATTGATACCCTGAAGCAAAACAAATCGTCTG
  GGAAGTGCACTATACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220050 representing NM_010260
  Red=Cloning site Green=Tags(s)
  
  MASEIHMSEPMCLIENTEAQLVINQEALRILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKRTGFSLGST
  VQSHTKGIWMWCVPHPKKAGQTLVLLDTEGLEDVEKGDNQNDCWIFALAVLLSSTFIYNSIGTINQQAMD
  QLHYVTELTDLIKSKSSPDQSGVDDSANFVGFFPTFVWTLRDFSLELEVNGKPVTSDEYLEHSLTLKKGA
  DKKTKSFNEPRLCIRKFFPKRKCFIFDRPAQRKQLSKLETLREEELCGEFVEQVAEFTSYILSYSSVKTL
  CGGIIVNGPRLKSLVQTYVGAISNGSLPCMESAVLTLAQIENSAAVQKAITHYEEQMNQKIQMPTETLQE
  LLDLHRPIESEAIEVFLKNSFKDVDQKFQTELGNLLVAKRDAFIKKNMDVSSARCSDLLEDIFGPLEEEV
  KLGTFSKPGGYYLFLQMRQELEKKYNQAPGKGLQAEAMLKNYFDSKADVVETLLQTDQSLTEAAKEVEEE
  RTKAEAAEAANRELEKKQKEFELMMQQKEKSYQEHVKKLTEKMKDEQKQLLAEQENIIAAKLREQEKFLK
  EGFENESKKLIREIDTLKQNKSSGKCTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010260
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010260.1, NP_034390.1
• RefSeq Size: 2471 bp
• RefSeq ORF: 1770 bp
• Locus ID: 14469
• UniProt ID: Q9Z0E6
• Cytogenetics: 3 66.69 cM
• MW: 66.7 kDa
• Gene Summary: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (By similarity). Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes (By similarity).[UniProtKB/Swiss-Prot Function]