Elmo2 (NM_080287) Mouse Tagged ORF Clone

CAT#: MR220040

Elmo2 (Myc-DDK-tagged) - Mouse engulfment and cell motility 2, ced-12 homolog (C. elegans) (Elmo2), transcript variant 3

Product Images

Circular map for MR220040

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elmo2
• Synonyms: 1190002F24Rik; CED-12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220040 representing NM_080287
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCTCCGTCTGACATTGTCAAAGTGGCCATCGAGTGGCCAGGTGCTAACGCCCAGCTCCTTGAAA
  TTGACCAGAAACGGCCGCTGGCATCCATCATCAAGGAGGTGTGCGATGGGTGGTCACTGCCGAACCCGGA
  GTACTACACCCTCCGCTATGCAGATGGGCCTCAGCTCTACGTCACGGAGCAGACCCGAAATGACATTAAG
  AACGGGACAATCTTACAACTGGCCGTCTCCCCGTCCCGGGCTGCACGCCAGCTGATGGAAAGGACCCAGT
  CATCTAGTATGGAGACCCGGCTGGATGCCATGAAGGAGTTGGCTAAGCTCTCAGCTGACGTGACTTTCGC
  CACGGAGTTCATTAACATGGACGGCATCATTGTGCTGACGAGGCTCGTGGAGAGTGGGACCAAGCTCCTG
  TCCCACTACAGTGAGATGCTGGCATTCACCCTGACTGCCTTCTTAGAGCTCATGGATCATGGCATTGTCT
  CCTGGGACATGGTTTCAGTCACCTTTATTAAGCAGATTGCAGGGTACGTGAGCCAGCCCATGGTCGATGT
  GTCCATCCTCCAGCGGTCCCTGGCCATCCTAGAGAGCATGGTACTAAACAGCCAGAGCCTGTACCAGAAG
  ATAGCGGAGGAGATCACCGTGGGACAGCTCATCTCCCACCTGCAGGTCTCCAACCAGGAGATCCAGACCT
  ACGCCATTGCTCTGATTAACGCGCTGTTCCTGAAGGCCCCCGAAGACAAGAGACAGGACATGGCCAATGC
  CTTTGCACAGAAGCACCTTCGGTCCATAATCCTGAACCATGTGATCAGAGGGAATCGTCCAATCAAAACA
  GAGATGGCCCATCAGCTGTATGTCCTTCAGGTCTTGACCTTTAACCTTCTGGAAGAAAGAATGATGACCA
  AGATGGATCCCAATGACCAGGCTCAGAGAGACATTATATTTGAACTGAGGAGGATTGCCTTCGACGCAGA
  GTCTGACCCCAGCAACGTCCCCGGGAGTGGGACTGAAAAGCGCAAGGCCATGTATACCAAGGACTATAAA
  ATGCTGGGCTTCACCAACCATATCAACCCAGCCTTGGACTTCACCCAGACTCCTCCTGGAATGCTGGCGC
  TGGACAACATGCTGTACCTGGCTAAAGTCCACCAGGACACCTACATCCGGATCGTGCTGGAGAACAGCAG
  TCGGGAGGACAAACACGAGTGTCCGTTCGGCCGCAGTGCCATCGAGCTCACCAAGATGCTCTGTGAGATC
  CTGCAGGTCGGGGAGCTCCCTAATGAAGGGCGCAATGACTACCACCCCATGTTCTTCACCCACGACCGAG
  CCTTCGAGGAACTCTTCGGGATCTGCATCCAGCTGCTGAACAAGACCTGGAAGGAGATGAGGGCGACAGC
  CGAGGATTTCAACAAGGTTATGCAAGTTGTCCGAGAGCAGATCACCCGGGCTCTGCCCTCTAAACCCAAC
  TCTTTGGATCAGTTCAAGAGTAAACTTCGTAGCCTGAGCTACTCAGAAATTCTGCGGTTGCGCCAGTCTG
  AGAGGATGAGCCAGGATGATTTCCAGTCCCCACCAATTGTGGAGCTTCGAGAGAAGATACAGCCTGAGAT
  CCTGGAGCTGATCAAGCAACAGCGCCTCAACCGGCTATGCGAGGGCAGCAGCTTCCGGAAAATCGGGAAC
  CGTCGTCGGCAAGAGAGGTTCTGGCACTGCCGCTTGGCACTGAACCACAAGGTTTTGCATTACGGTGACT
  TGGATGACAACCCTCAAGGGGAGGTGACATTCGAATCCCTGCAGGAGAAAATTCCTGTTGCAGATATTAA
  GGCCATTGTTACTGGAAAAGACTGTCCTCACATGAAAGAGAAGAGTGCCCTGAAACAGAACAAGGAGGTG
  TTGGAATTGGCCTTCTCCATCCTGTATGATCCTGATGAGACACTGAATTTCATTGCTCCCAACAAGTATG
  AGTACTGCATCTGGATTGATGGACTCAGCGCTCTCCTGGGGAAGGATATGTCCAGCGAGCTAACCAAGAG
  CGACTTGGACACGCTGCTGAGCATGGAGATGAAGCTGCGGCTTCTGGACTTGGAGAACATTCAGATCCCC
  GAGGCGCCACCTCCGGTCCCCAAGGAGCCCAGCAGCTATGACTTCGTCTATCACTATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220040 representing NM_080287
  Red=Cloning site Green=Tags(s)
  
  MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLPNPEYYTLRYADGPQLYVTEQTRNDIK
  NGTILQLAVSPSRAARQLMERTQSSSMETRLDAMKELAKLSADVTFATEFINMDGIIVLTRLVESGTKLL
  SHYSEMLAFTLTAFLELMDHGIVSWDMVSVTFIKQIAGYVSQPMVDVSILQRSLAILESMVLNSQSLYQK
  IAEEITVGQLISHLQVSNQEIQTYAIALINALFLKAPEDKRQDMANAFAQKHLRSIILNHVIRGNRPIKT
  EMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNVPGSGTEKRKAMYTKDYK
  MLGFTNHINPALDFTQTPPGMLALDNMLYLAKVHQDTYIRIVLENSSREDKHECPFGRSAIELTKMLCEI
  LQVGELPNEGRNDYHPMFFTHDRAFEELFGICIQLLNKTWKEMRATAEDFNKVMQVVREQITRALPSKPN
  SLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELREKIQPEILELIKQQRLNRLCEGSSFRKIGN
  RRRQERFWHCRLALNHKVLHYGDLDDNPQGEVTFESLQEKIPVADIKAIVTGKDCPHMKEKSALKQNKEV
  LELAFSILYDPDETLNFIAPNKYEYCIWIDGLSALLGKDMSSELTKSDLDTLLSMEMKLRLLDLENIQIP
  EAPPPVPKEPSSYDFVYHYG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080287
• ORF Size: 2160 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080287.2, NP_525026.2
• RefSeq Size: 4600 bp
• RefSeq ORF: 2163 bp
• Locus ID: 140579
• UniProt ID: Q8BHL5
• Cytogenetics: 2 H3
• MW: 83 kDa
• Gene Summary: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function]