Adam5 (NM_007401) Mouse Tagged ORF Clone

CAT#: MR220009

Adam5 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 5 (Adam5)

Product Images

Circular map for MR220009

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam5
• Synonyms: tMDC; tMDC II; tMDCII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220009 representing NM_007401
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTCCTTCTTTTGCTCTTTTTGCATCTTAAAGGGCTGCAGGCAGGCCAGAATCCTCAGAAAACCA
  CCCTGCAGACAACTGTTCCTGAAAAGATCTCATCACCGGATGTGGAAACAGATGCAGAAGACCATATGGC
  TTATCTTATTACAATAAATGAAACCCCCCATTTCATCCATCTTAAAAAACAATCATTTATAACTCCAACT
  GCAGTTGTTTATACTTACGACAGAAATGATGTTCAACATTCTCAACCTTTGTCAGCTCTGGAAAATTGCA
  ATTACAATGGATATGTTGCAGGTTTCCCAAACTCTATCGTGACCCTCACCGTTTGCACAGGACTCAGGGG
  AATCATACAGTTTGAAAATGTCTCCTATGCAATCGAGCCCGTGGAAACCCTGTCAGGATTTGTGCACGTG
  ATCTATGAGAACACAAACAAACATGCTGTGATTCCTGACCTGGGAAAGAATCAGTCATACAGTTGGTTTG
  ACGAATCAGATTATCAATTCCGGAGTAACATGAAGAAATCTGGATTTACCGTGTTACGCCAACGATTCAT
  TATGATGGATATCATCGTGGATAAAAAGCTGTTTGATTACATGGGTTCTGACACTGAAGTTGTGCTACAG
  AAAGTCATTCAGATCATCGGTTTCGTTAACACTATGCTCTCCAAGTTAAAACTGACTGTCTTAATAAATT
  CCATTGAAATCTGGTCGAAGGAAAACAGAATTCGTTTATCAAAGGCAGTTGATGACTTATTGGTTCAGTT
  CTCAATTTGGAAACATGAGTATAGATCACAACATGTTGCATACTTACTGGCATTTGAGGAGCACCCTGCT
  TCCACGGGGGCTCTATATCCAGGGAATCTGTGCAAACTGGAGTACAATGCTGCTGTTGCCCTGTATCCAA
  AAGGTTTATCGTTAGAATCGTTTAGTGTCATTGTCCTGCAGCTGCTGAGCATTGGTATGGGCTTAACTTA
  CGACACTGAGAACTGCCACTGTACAGGAGAAGTTTGCCTAATGACGCCCAAAGCAATATATTCTGGGGGT
  GTGAAGGACTTCAGTACTTGTACCCTGGATGACTTTAAATACCTGTCAACAAGACAAGACCTTAGATGTC
  TCCAGGACTTGCCTTTGGAAAGAAAGCCAGCTCGCAGGCCAAGGAGAATCTGTGGCAATGGGATACTGGA
  GATGAACGAGCAATGTGACTGTGGCACTTTGAAGAACTGTACACATAGAAAATGCTGTGACCCTATGTCA
  TGTCGATTGAAAAACAAGGCCACGTGTGGTTCTGGGGAGTGCTGTAGTCAAGATTGCACGGTAAAAATGA
  ATGATGTGGTCTGTAGAAAGTCGGTAGATGAATGTGACTTTGTGGAGTACTGTAACGGAAAAGATCCCTA
  TTGTGTCCCTAACACATATGCACGCAATGGGCAGTATTGTGAATCAGGTGAAGCCTTCTGTTTTGAAGGA
  CGATGTCAGACTGCTGACAAGCAATGTATGAGCATGCTTGGGAAATACGTGAGAGGGGCTTCCTTTGCGT
  GCTATGAAGAGTTCAATTCCAGAGGCGATAGATTTGGCAACTGTATTCATAATTTTTGTGCTTTCAGAAA
  TTCTCTCTGTGGGAAACTAATCTGTACGTGGCCATTCAAGAAGCTGGTGCTAAAAGCCAATTTATCTGTG
  GCCTATGCGCAAATCCGGGATGACTTGTGTGTAGCTATGTATAAAGGTGGGAGGATACCTAAGACAACAA
  AGACGACATATTCCAATCCAGCGGACAGAGATGAGACATTTGTAAACGACGGCACCATCTGTGGCCCTGA
  TATGTTTTGTTTGAGAGCATCATGCACAGAAACTAGATTTCACATGGATTCTTCCAAGTGTGATTCAACT
  AGAGATTGCAACGACCATGGGGTTTGCAACAATTTACAGCATTGCCACTGTGATATAGGATATAACCCTC
  CTTTTTGTGAGGAACATAAGGGACAGTTTGGAAGCGTTGATGATGGACATAAATATCATGTTGAAGATGG
  TAAAAGCTATAAGCAGCAAAGTCATAGTAATCTTAAAAAAAATCAGCTTCAGCTCATTCTCTACATATCT
  CTGCCCTTACTTGTGATGATTTCAGCTGTAGTTATAAAGCAAAGCAAACTGAGTCGTGTATGTGACAGAG
  AAAGAAGTGAAAGCGACAGCTCTACAACAGAAGATAGCGGGAGTAACACCAATGTGACCAGCAGTGGAGG
  AAGCACCTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220009 representing NM_007401
  Red=Cloning site Green=Tags(s)
  
  MFLLLLLFLHLKGLQAGQNPQKTTLQTTVPEKISSPDVETDAEDHMAYLITINETPHFIHLKKQSFITPT
  AVVYTYDRNDVQHSQPLSALENCNYNGYVAGFPNSIVTLTVCTGLRGIIQFENVSYAIEPVETLSGFVHV
  IYENTNKHAVIPDLGKNQSYSWFDESDYQFRSNMKKSGFTVLRQRFIMMDIIVDKKLFDYMGSDTEVVLQ
  KVIQIIGFVNTMLSKLKLTVLINSIEIWSKENRIRLSKAVDDLLVQFSIWKHEYRSQHVAYLLAFEEHPA
  STGALYPGNLCKLEYNAAVALYPKGLSLESFSVIVLQLLSIGMGLTYDTENCHCTGEVCLMTPKAIYSGG
  VKDFSTCTLDDFKYLSTRQDLRCLQDLPLERKPARRPRRICGNGILEMNEQCDCGTLKNCTHRKCCDPMS
  CRLKNKATCGSGECCSQDCTVKMNDVVCRKSVDECDFVEYCNGKDPYCVPNTYARNGQYCESGEAFCFEG
  RCQTADKQCMSMLGKYVRGASFACYEEFNSRGDRFGNCIHNFCAFRNSLCGKLICTWPFKKLVLKANLSV
  AYAQIRDDLCVAMYKGGRIPKTTKTTYSNPADRDETFVNDGTICGPDMFCLRASCTETRFHMDSSKCDST
  RDCNDHGVCNNLQHCHCDIGYNPPFCEEHKGQFGSVDDGHKYHVEDGKSYKQQSHSNLKKNQLQLILYIS
  LPLLVMISAVVIKQSKLSRVCDRERSESDSSTTEDSGSNTNVTSSGGSTSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007401
• ORF Size: 2253 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007401.3, NP_031427.2
• RefSeq Size: 2791 bp
• RefSeq ORF: 2256 bp
• Locus ID: 11499
• UniProt ID: Q3TTE0
• Cytogenetics: 8 13.26 cM
• MW: 85 kDa
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of related ADAM genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]