Akirin2 (NM_001007589) Mouse Tagged ORF Clone

CAT#: MR219891

Akirin2 (Myc-DDK-tagged) - Mouse akirin 2 (Akirin2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akirin2
• Synonyms: 2700059D21Rik; AA114675; AA522011; Akirin-2; AU019887
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219891 representing NM_001007589
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGCGGAGCCACTCTGAAAAGGACTCTGGATTTCGACCCGCTGCTGAGCCCCGCATCTCCGAAGC
  GGAGGCGGTGCGCGCCTTTGTCGGCGCCGGCTTCGGCTGCCGCCTCCCCTGCCGCCGCCACCGCCGCCGC
  CGCCGCCTCGGCCGCGGCCGCCTCGCCGCAGAAGTATCTCCGGATGGAGCCTTCCCCCTTCGGCGACGTC
  TCTTCCCGGCTCACCACAGAACAAATTCTGTACAACATAAAACAGGAATATAAACGCATGCAGAAGAGAA
  GACATTTAGAAGCTAGTTTTCAGCAGGCAGATCCAGGTTGTACTTCCGATTCACAGCCACATGCATTTCT
  CATCAGTGGACCAGCATCACCAGGGACTTCATCTGCAACATCCTCACCATTAAAAAAAGAACAGCCCTTA
  TTTACTCTAAGGCAGGTTGGAATGATCTGTGAACGTTTGTTGAAAGAACGGGAAGAGAAAGTTCGAGAAG
  AATATGAAGAAATACTGAATACAAAACTTGCAGAACAATATGATGCTTTTGTGAAGTTTACGCATGATCA
  GATAATGCGGCGATATGGAGAACAGCCTGCTAGTTATGTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219891 representing NM_001007589
  Red=Cloning site Green=Tags(s)
  
  MACGATLKRTLDFDPLLSPASPKRRRCAPLSAPASAAASPAAATAAAAASAAAASPQKYLRMEPSPFGDV
  SSRLTTEQILYNIKQEYKRMQKRRHLEASFQQADPGCTSDSQPHAFLISGPASPGTSSATSSPLKKEQPL
  FTLRQVGMICERLLKEREEKVREEYEEILNTKLAEQYDAFVKFTHDQIMRRYGEQPASYVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007589
• ORF Size: 603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007589.3, NP_001007590.2
• RefSeq Size: 1729 bp
• RefSeq ORF: 606 bp
• Locus ID: 433693
• UniProt ID: B1AXD8
• Cytogenetics: 4 A5
• MW: 22.6 kDa
• Gene Summary: Forms a complex with YWHAB that acts to repress transcription of DUSP1 (By similarity). Required for embryonic development and the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6.[UniProtKB/Swiss-Prot Function]