Tmprss11b (NM_177024) Mouse Tagged ORF Clone

CAT#: MR219844

Tmprss11bnl (Myc-DDK-tagged) - Mouse transmembrane protease, serine 11b N terminal like (Tmprss11bnl)

Product Images

Circular map for MR219844

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmprss11b
• Synonyms: 9930019B18Rik; Tmprss11bnl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219844 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGACCAGTCATAGCATCCCGCAAATCTATCCCACCATGGCTAATCATCCTCTGTGTGCTTGGAG
  TGCTGGCAGCCCTTGGGATAATTATTGGACTCTTAGTTCACTTTCTGGCAGTAGAAAACAAGATCTACTA
  CTATCAAGGTGGTTTTAAAGTGCTGGATATTCCATATGACAGAAATTATGAAAGGGAGACATCACTAGAA
  AGTAACTACCTCAGTAAAATCCTTGAGAATAAAATGGTTGAAGCCTTTCAGAATTCTAACATTTACAAAC
  AATATATCAACTCTCAGGTCATCACATTGGTTCCTGATAACAACAGCGTGACAGCACATATATGGCTGGT
  GTTTAAGGATCCCTGGTCTAACAAGGAAAACCTAAGAAGGAGAATTGAAAGCATCTTACGTCAGATGCTG
  GAGAACAACCCAGAATCCCTGACTACAGATCCTGGGTCTCTGAAGCTCACGGAAATCAGTAAGGTTGATG
  CTGAGAAGATTATCAACAACCGCTGTGGGAGAAGACCAAGAATGTCTGCAACCTACGACAGAATCACGGG
  AGGTTCCACTGCTCACAAAGGAGAGTGGCCATGGCAAGCGAGTCTCAGAGTGAATGGTAAACACTACTGT
  GGGGCATCTCTGATTGGCGAAAGATTTCTGCTCACAGCAGCTCATTGCTTTCAAGGGACAAATAATCCAA
  AAAACTTAACTGTCAGCTTTGGCACTAGAGTAACTCCAGCCTACATGCAGCATTCTGTTCAAGAGATTAT
  TATTCATGAAGATTATGTGAAGGGGGAACATCATGATGACGTTGCAGTTATAAAGCTCACTGAAAAAGTT
  TCATTCAATAATGATGTGCATCGGGTTTGTCTTCCGGAATCCACCCAGATTTTTCCACCAGGTGAAGGAG
  TCGTTGTTACAGGATGGGGATCATTTTCTTACAATGGTAAATCCCCACTGTTACTTCAGAAAGCATCCAT
  TAAAATCATTGACACAAATACTTGCAATTCAGAAGAAGCTTATGGAGGCAGGATAGTGGACACAATGCTC
  TGTGCTGGCTACTTGGAAGGCAGTATCGATGCTTGCCAGGGTGACTCTGGAGGACCACTAGTTCATCCCA
  ATTCTCGAGATATTTGGTACCTTGTTGGGATAGTGAGCTGGGGGCATGAATGTGGCAGAGTCAACAAGCC
  AGGGGTCTATATGAGAGTAACTTCCTACCGTAACTGGATTGCCTCCAAGACTGGTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219844 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYRPVIASRKSIPPWLIILCVLGVLAALGIIIGLLVHFLAVENKIYYYQGGFKVLDIPYDRNYERETSLE
  SNYLSKILENKMVEAFQNSNIYKQYINSQVITLVPDNNSVTAHIWLVFKDPWSNKENLRRRIESILRQML
  ENNPESLTTDPGSLKLTEISKVDAEKIINNRCGRRPRMSATYDRITGGSTAHKGEWPWQASLRVNGKHYC
  GASLIGERFLLTAAHCFQGTNNPKNLTVSFGTRVTPAYMQHSVQEIIIHEDYVKGEHHDDVAVIKLTEKV
  SFNNDVHRVCLPESTQIFPPGEGVVVTGWGSFSYNGKSPLLLQKASIKIIDTNTCNSEEAYGGRIVDTML
  CAGYLEGSIDACQGDSGGPLVHPNSRDIWYLVGIVSWGHECGRVNKPGVYMRVTSYRNWIASKTGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177024
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177024.4, NP_795998.2
• RefSeq Size: 2216 bp
• RefSeq ORF: 1251 bp
• Locus ID: 319875
• UniProt ID: Q14C59
• Cytogenetics: 5 E1
• MW: 46.7 kDa
• Gene Summary: Serine protease.[UniProtKB/Swiss-Prot Function]