Bfar (NM_001177552) Mouse Tagged ORF Clone

USD 68.00

USD 149.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Bfar (NM_001177552) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Bfar
Synonyms 3010001A07Rik; 3110001I22Rik; AI666707; AW107665; Bar; Rnf47
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR219635 representing NM_001177552
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGCCTCAGAAAAATGACCTGAGCATGAGAGAGCAGGAGGAAGAGCATCCTGTCAGAAGCAGCG
GCCCTCAGATTTCTGTGAGCGAGTTCTCCTGCCACTGCTGCTACGACACCCTGGTTAACCCCACCACCTT
GAACTGTGGCCACAGCTTCTGCCGGCACTGCCTGGCTTTATGGTGGATGTCTTCAAAGAAGACAGAGTGT
CCAGAATGCAGAGAAAAATGGGAAGGTTTTCCTAAAGTCAACATTCTCCTCAGGTTGCTTTTAACTTTGA
CAGAAGAAGAGTTTTCCAGGGCACCTTATACCATAGAGAACAGTAGCCACAGAAGGGTCATCCTCACGGA
GCTGGAGCGTGTGAGAGCCCTGGGAGTGAAGCCACCGCAGAATCTCTGGGAGTACAAGGCTGTCAACCCG
GGTAGATCCCTATTCTTGCTCTACGCTCTCAAGAGCTCACCCAGACTTGGCTTGCTATACCTGTACCTGT
TTGACTACACAGACTGCTTCCTACCCTTCATCCACACCATCTGCCCTCTGCAGGAAAACAGCTCTGGCGA
GGACATTTTCACCAAGCTTCTGGACCTGAGGGAGCCCACGTGGAAGCAGTGGAGAGAATTCCTCGTCAAG
TACTCCTTTCTTCCGTACCAGCTGATCGCGGAGTTTGCCTGGGACTGGCTGGAGGTCCACTACTGGACAT
CTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTTCTCTTTTTGGAGGATCTGGTC
GAGAAGTGAGCTGAAGACAGTGCCCCAGAGGATGTGGAGCCACTTCTGGAAAGTATCGACACAGGGGCTT
TTCATGGCCATGTTCTGGCCGCTTATTCCTCAGTTTGTCTGCAACTGTTTATTTTACTGGGCTCTGTACT
TCAACCCAATTATTAACATTGATCTGGTGGTCAAGGAAGTCCGACGGCTGGAAACCCAAGTGTTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR219635 representing NM_001177552
Red=Cloning site Green=Tags(s)

MEEPQKNDLSMREQEEEHPVRSSGPQISVSEFSCHCCYDTLVNPTTLNCGHSFCRHCLALWWMSSKKTEC
PECREKWEGFPKVNILLRLLLTLTEEEFSRAPYTIENSSHRRVILTELERVRALGVKPPQNLWEYKAVNP
GRSLFLLYALKSSPRLGLLYLYLFDYTDCFLPFIHTICPLQENSSGEDIFTKLLDLREPTWKQWREFLVK
YSFLPYQLIAEFAWDWLEVHYWTSRFLIVNAVLLSVLELFSFWRIWSRSELKTVPQRMWSHFWKVSTQGL
FMAMFWPLIPQFVCNCLFYWALYFNPIINIDLVVKEVRRLETQVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001177552
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001177552.1, NP_001171023.1
RefSeq Size 2635
RefSeq ORF 978
Locus ID 67118
Cytogenetics 16 A1
MW 39.2 kDa

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