Awat2 (NM_177746) Mouse Tagged ORF Clone

CAT#: MR219385

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  • TrueORF®

Awat2 (Myc-DDK-tagged) - Mouse acyl-CoA wax alcohol acyltransferase 2 (Awat2)



  "NM_177746" in other vectors (4)

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USD 350.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Awat2 (NM_177746) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Awat2
Synonyms 9430062J17Rik; ARAT; Dgat2l4; WS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR219385 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTGGCCCACCAAGAAGGACCTCAAGACCGCCATGGAGGTCTTTGCTCTTTTCCAGTGGGCCCTCA
GTGCCTTGGTTATAGTCACCACTGTGATCATTGTCAACCTCTACTTGGTGGTGTTCACATCTTACTGGCC
TGTCACCGTGTTGATGCTCACCTGGCTGGCTTTTGACTGGAAGACCCCTGAGCGAGGTGGCCGCAGGTTC
ACCTGTGTGAGGAAGTGGCGTCTGTGGAAACACTATAGCGACTACTTCCCACTCAAGATGGTGAAGACGA
AAGATATTTCACCTGACCGCAACTACATCCTTGTCTGCCATCCTCATGGGCTCATGGCCCATTCATGTTT
TGGTCACTTTGCCACTGACACAACAGGCTTTTCCAAGACCTTTCCTGGTATCACTCCTTATATGCTCACA
CTAGGAGCCTTTTTCTGGGTACCTTTCCTCAGAGATTATGTAATGTCTACAGGGTCGTGCTCTGTGAGTA
GATCCTCCATGGACTTTTTGCTTACCCAGAAGGGCACAGGCAACATGCTTGTGGTGGTGGTTGGTGGCCT
GGCTGAGTGCAGATACAGCACGCCAGGCTCTACCACCCTGTTCTTGAAGAAGCGGCAAGGCTTTGTGCGC
ACAGCCCTTAAACATGGGGTGTCTCTAATCCCAGCTTATGCCTTTGGAGAGACAGACCTCTATGACCAGC
ACATTTTCACTCCTGGGGGCTTTGTCAATCGCTTCCAGAAGTGGTTCCAGAAGATGGTACACATCTACCC
CTGTGCTTTCTATGGGCGTGGCCTCACCAAGAACTCCTGGGGCCTTCTGCCCTATTCTCAGCCAGTAACC
ACTGTTGTTGGAGAACCTCTACCATTGCCCAAGATTGAGAATCCGAGCGAGGAGATTGTGGCCAAATACC
ACACACTCTATATTGATGCCCTACGAAAATTGTTTGATCAGCATAAGACCAAGTTTGGCATCTCAGAGAC
CCAGGAGCTGGTGATAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR219385 protein sequence
Red=Cloning site Green=Tags(s)

MFWPTKKDLKTAMEVFALFQWALSALVIVTTVIIVNLYLVVFTSYWPVTVLMLTWLAFDWKTPERGGRRF
TCVRKWRLWKHYSDYFPLKMVKTKDISPDRNYILVCHPHGLMAHSCFGHFATDTTGFSKTFPGITPYMLT
LGAFFWVPFLRDYVMSTGSCSVSRSSMDFLLTQKGTGNMLVVVVGGLAECRYSTPGSTTLFLKKRQGFVR
TALKHGVSLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQKMVHIYPCAFYGRGLTKNSWGLLPYSQPVT
TVVGEPLPLPKIENPSEEIVAKYHTLYIDALRKLFDQHKTKFGISETQELVIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_177746
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_177746.3, NM_177746.4, NP_808414.2
RefSeq Size 1632 bp
RefSeq ORF 1002 bp
Locus ID 245532
Cytogenetics X C3
MW 38.1 kDa

Citations (1)

Other Versions

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