Col14a1 (NM_181277) Mouse Tagged ORF Clone

CAT#: MR218889

Col14a1 (Myc-DDK-tagged) - Mouse collagen, type XIV, alpha 1 (Col14a1)

Product Images

Circular map for MR218889

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col14a1
• Synonyms: 5730412L22Rik; AW108078
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218889 representing NM_181277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATTTGGCAGTGCAAGATGCGGGACTGGCTGATTCTAGCTTTTTTGGCGGCTGCTTGTTTCTGCA
  CCATTGTCAGGGGACAAGTGGCTCCACCCACAAGGTTAAGATATAATGTGATTTCCCATGATAGTATACA
  GATTTCATGGAAAGCACCAAGAGGGAAATTTGGTGGCTACAAACTGCTCGTGGCTCCAGCTTCAGGAGGA
  AAGACCAACCAGATGAATCTCCAGAACACTGCAACAAAAGCGATTATTCAAGGCCTTCTGCCAGAGCAGA
  ACTACACAGTTCAACTCATTGCCTACTATAAAGACAAGGAAAGCAAGCCCGCTCAAGGCCAGTTCAGAAT
  TAAAGATTTAGAGAAAAGAAAGGATCCAACAAAGCCCAAAGTCAAGGTTGTGGACAAAGGAAATGGAAGT
  AAACCAACTTCACCAGAAGAGGTAAAATTCTTCTGTGAGACTCCAGCCATCGCTGACATCGTCATCCTGG
  TGGATGGGTCGTGGAGTATTGGGAGGTTCAACTTCAGGCTTGTGCGCAATTTCTTGGAAAACCTGGTCAC
  AGCGTTCAACGTGGGCTCAGAAAAGACAAGAATCGGCTTGGCACAGTACAGTGGGGACCCCAGAATAGAG
  TGGCACTTGAATGCCTTTAACACAAAAGATGAGGTGATTGATGCTGTCCGAAGCCTTCCATACAAAGGAG
  GAAATACCCTAACAGGTCTTGCTTTGAACTTTATTTTTGAGAATAGCTTTAAACCAGAAGCAGGATCGAG
  GAGTGGAGTTTCCAAGATTGGCATTTTGATCACAGATGGAAAATCCCAGGATGATATTATCCCACCATCT
  AGAAACCTCCGTGAGTCTGGTGTAGAGCTGTTTGCCATAGGGGTTAAAAATGCCGACCTGAGTGAGCTGC
  AGGAGATCGCCTCTGAGCCAGACAGCACCCATGTGTACAATGTTGCTGAGTTCGACCTGATGCACACAGT
  GGTGGAGAGCCTGACCCGGACTGTATGCTCTAGAGTGGAAGAGCAGGACAAGGAGATTAAAGCCTCAGCC
  CTTGCCACCATCGGGCCACCTACAGAGCTGATCACTTCTGAAGTCACTGCCAGGAGCTTTATGGTTAACT
  GGACTCAGTCCCCAGGAAAAGTGGAAAAATACAGAGTTGTGTATTATCCCACCAGAGGCGGAAAGCCGGA
  GGAGGTGGTGGTGGATGGGAGTGTATCTTCCACAGTGTTGAAAAACTTGATGTCTTCAACTGAGTACCAG
  ATAGCAGTGTTTGCAGTATCTGCCCACACAGCTAGTGAAGGCCTGCGGGGAGCAGAGACCACATTGGCCT
  TGCCCATGGCTTCCGACCTCGAGCTGTACGACGTGACTGAGAACAGCATGCGCGTCAGGTGGGATGCAGT
  ACCTGGAGCCACAGGCTATCTGATCCTCTATGCTCCTCTCACAGAAGGTCTGGCTGGAGATGAGAAAGAG
  ATGAAAATTGGAGAGACACACACAGACATTGAACTGAGTGGGCTATTCCCCAATACAGAATATACAGTCA
  CTGTGTATGCCATGTTTGGAGAAGAGGCCAGCGATCCTGCTACAGGACAAGAGACAACCCTACCCTTAAC
  CCCACCAAGAAATTTGCGGATCTCCAATGTTGGCTCTAACAGCGCTCGGTTAACCTGGGACCCCGCATCG
  GGAAAGATCAGTGGTTACAGAATTGTGTATACCAGTGCAGACGGGACAGAAATCAATGAGGTTGAAGTCG
  ATCCTATCACTACATTCCCTCTGAAAGGCTTGACCCCCCTCACGGAGTACTCCATTGCAATCTTCTCCAT
  CTACGAGGAGGGGCAGTCCTTGCCCCTGGTTGGAGAGTTTACCACAGAGGAAGTTCCCGCCCAGCAATAC
  TTGGAAATTGATGAGGTGAAGACAGACAGTTTTCGAGTGACTTGGCATCCGCTCTCCGCTGAGGAGGGGC
  AGCACAAGCTGATGTGGATTCCGGTCTATGGCGGGAAGACCCAGGAGGTTGACCTCAAAGAAGAACAGGA
  CTCTTATGTTATTGAAGGCCTGGATCCTGGAACCGAGTACGAAGTCTCCCTGTTAGCTGTGCTTGATGAC
  GGAAGCGAGAGTGAGGTGGTGACCGCAGTGGGAACCACACTTGATGATTTTTGGACTGAAGCACCCACAG
  CCATAGAACCTACCAGCCCTGTGACTTCAGTTCTCCAGACAGGAATCAGAAACCTGGTTGTAGATGACGA
  AACTGCTACTAGCCTGCGGGTATCGTGGGACATTTCTGACAGCAATGTGGAGCAGTTTAGGGTGACCTAC
  CTGAAAGCCCAAGGGGACCCTATGGAAGAAGTGGTTATGGTGCCTGGAGTGCAAAACAGTCTCCTTCTGA
  AGGCTCTACTTCCAGACACTGAGTATAAAGTCACAGTAACCCCTGTCTATACTGTTGGAGAAGGCGTCAG
  CGTCTCCGCTCCAGGGAAGACTTTGCCCTCCTCTGGTCCTCAAAACCTGAGGGTCTCAGAAGAATGGTAC
  AACCGGGTGCGCATTACATGGGACCCACCCTCTGGCCCCGTGAAGGGCTATAGGATTGTCTACAAACCTG
  TGAGTGTCCCTGGTCAAACTCTGGAGACCTTCGTGGGTGCTGACATCAACACCATTGTCATGACAAATCT
  CCTCAGTGGAATGGACTACAATGTGAAGATCTTTGCTTCCCAGGCGTCAGGCTTCAGTGATGCTCTGACT
  GGCCTCGTGCAAACACTGTTCTTAGGGGTGACAGATCTCCAAGCCAATCAGGTTGAAATGACAAGCTTGT
  GTGCTCGGTGGCAAATACATCGCCATGCCACAGCCTACAGGATAGTTCTAGAATCCCTTCAGGACACACA
  AGCACAGGAATCCACGGTGGGAGGAGGCGTAAACAGGCATTGCTTCTATGGACTTCAGCCTGATTCAGAG
  TATAAAATCAGTGTTTACACAAAGCTCCAGGAGCTAGAGGGACCCAGTGTGAGCATCATGCAGAAAACAC
  AGTCACTTCCCACGGAACCACCGACTTTTCCTCCAACCATCCCACCAGCAAAGGAAGTATGTAAAGCTGC
  CAAGGCAGACCTGGTATTCATGGTGGATGGATCTTGGAGCATAGGAGATGACAATTTCAATAAAATCATC
  AATTTTCTATACAGCACTGTGGGAGCCCTGGACAAGATTGGTGCAGATGGAACTCAGGTGGCAATGGTTC
  AGTTCACTGATGACCCCCGAACAGAATTCAAACTTGATTCTTACAAAACCAAAGAGACCCTTCTTGATGC
  AATTAGACACATTTCCTACAAAGGAGGAAATACTAAAACAGGAAAAGCTATCAAGCATGTTCGGGATACC
  TTATTTACATCAGACTCAGGGACAAGGAGAGGCATCCCAAAGGTCATCGTGGTGATAACTGATGGACGGT
  CACAAGATGATGTCAACAAAATCTCCAGGGAGATGCAGGCAGATGGTTTCAACATCTTTGCCATCGGTGT
  GGCTGACGCTGACTACTCCGAGTTGGTTCAGATCGGCAGCAAGCCCAGCTCACGGCATGTCTTCTTTGTG
  GATGACTTTGATGCCTTTAAGAAAATTGAAGATGAATTGATTACTTTTGTCTGTGAAACTGCATCAGCAA
  CCTGCCCAATGGTGCACAAGGATGGAGTTGATCTTGCAGGGTTTAAGATGATGGAAATGTTTGGTTTGGT
  TGAAAAGGACTTTTCTGCAGTGGAAGGAGTATCTATGGAACCTGGTACCTTCAATTTGTTCCCATGCTAT
  CAAATCCATAAGGATGCTCTGGTTTCTCAGCCAACAAAGTATCTGCACCCAGAAGGATTGCCTTCTGACT
  ATACAATGAGTTTCCTGTTCCGGATTCTTCCTGACACACCACAGGAGCCATTTGCTCTTTGGGAGATTTT
  AAACAAAAATTCTGAGCCGCTAGTTGGGATCATATTGGACAATGGTGGGAAAACTCTAACTTATTTCAAC
  TATGACTATACTGGAGATTTTCAAACTGTCACTTTTGAAGGACCCGACATCAGGAAAATGTTCTATGGAA
  GCTTTCACAAGCTCCATGTTGTTGTCAGCAAGACTCTGGCCAAAGTGGTTGTTGACTGCAAGGAAGTTGG
  TCAGAAGGCAATAAATGCATCAGCGAATATCACATCGGATGGTGTGGAAGTACTAGGAAGAATGGTCAGA
  TCAAGAGGACCAAATGGAAACTCTGCACCATTCCAGTTACAAATGTTTGATATCGTTTGCTCTACATCAT
  GGGCCAGTAAAGACAGATGCTGCGAACTTCCTGGTCTGCGGGATGAAGAGTCCTGCCCAGACCTTCCACG
  GTCCTGCTCTTGCTCGGAAACCAATGAAGTGGCTCTAGGACCAGCTGGCCCCCCAGGTGGTCCAGGACTC
  CGAGGACCAAAAGGCCAACAAGGTGAACAGGGTCCGAAGGGACCAGAGGGACCTCGGGGAGAAACAGGTC
  CTGCAGGACCCCAGGGCCCCCCTGGACCCCAGGGACCTAGTGGTCTGTCCATTCAAGGCATGCCTGGAAT
  GCCGGGTGATAAAGGAGATAAAGGAGATGCTGGTCTTCCTGGTCCACAGGGTGTCCCTGGAGGTGTTGGT
  TCACCAGGCCGTGATGGCTCACCAGGCCAGAGGGGATTTCCCGGGAAGGACGGTTCCTCTGGGCCTCCAG
  GACCACCTGGGCCAATTGGCATTCCTGGAGCCCCTGGTGTCCCAGGGATCACAGGCAGCATGGGACCGCA
  AGGAGCCCTGGGGCCACCTGGTGTCCCTGGAGCGAAGGGAGAGCGAGGTGAACGGGGAGACCTGCAGTCT
  CAAGCCATGGTGAGGGCGGTGGCACGTCAAGTATGCGAGCAGCTCATTCAGAGTCACATGGCCAGGTACA
  CAGCCATCCTCAACCAGATCCCCAGCCAGTCCTCATCTATCCGGACCATCCAGGGCCCTCCTGGGGAGCC
  CGGGAGACCAGGTTCACCTGGAACCCCTGGAGAACAAGGACCCCCAGGTACCCCAGGCTTCCCAGGAAAT
  GCAGGAGTTCCGGGGACCCCAGGAGAACGAGGTCTAACTGGTGTCAAAGGAGAGAAAGGAAATCCAGGCA
  TTGGAACTCAAGGCCCAAGAGGTCCCCCTGGGCCAGCAGGACCTTCAGGGGAAAGTCGTCCTGGAAGTCC
  TGGGCCACCTGGCTCTCCTGGACCAAGGGGTCCCCCGGGCCACCTGGGAGTACCTGGACCTCAGGGTCCT
  TCTGGCCAGCCTGGGTATTGTGACCCCTCCTCGTGCTCTGCTTATGGCGTTGGAGTGTCCCATCCTGATC
  AGCCTGAGTTCACACCGGTCCAGGACGAGCAGGAGGCCATGGATCTGTGGAGCGCAGGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218889 representing NM_181277
  Red=Cloning site Green=Tags(s)
  
  MMIWQCKMRDWLILAFLAAACFCTIVRGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVAPASGG
  KTNQMNLQNTATKAIIQGLLPEQNYTVQLIAYYKDKESKPAQGQFRIKDLEKRKDPTKPKVKVVDKGNGS
  KPTSPEEVKFFCETPAIADIVILVDGSWSIGRFNFRLVRNFLENLVTAFNVGSEKTRIGLAQYSGDPRIE
  WHLNAFNTKDEVIDAVRSLPYKGGNTLTGLALNFIFENSFKPEAGSRSGVSKIGILITDGKSQDDIIPPS
  RNLRESGVELFAIGVKNADLSELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTVCSRVEEQDKEIKASA
  LATIGPPTELITSEVTARSFMVNWTQSPGKVEKYRVVYYPTRGGKPEEVVVDGSVSSTVLKNLMSSTEYQ
  IAVFAVSAHTASEGLRGAETTLALPMASDLELYDVTENSMRVRWDAVPGATGYLILYAPLTEGLAGDEKE
  MKIGETHTDIELSGLFPNTEYTVTVYAMFGEEASDPATGQETTLPLTPPRNLRISNVGSNSARLTWDPAS
  GKISGYRIVYTSADGTEINEVEVDPITTFPLKGLTPLTEYSIAIFSIYEEGQSLPLVGEFTTEEVPAQQY
  LEIDEVKTDSFRVTWHPLSAEEGQHKLMWIPVYGGKTQEVDLKEEQDSYVIEGLDPGTEYEVSLLAVLDD
  GSESEVVTAVGTTLDDFWTEAPTAIEPTSPVTSVLQTGIRNLVVDDETATSLRVSWDISDSNVEQFRVTY
  LKAQGDPMEEVVMVPGVQNSLLLKALLPDTEYKVTVTPVYTVGEGVSVSAPGKTLPSSGPQNLRVSEEWY
  NRVRITWDPPSGPVKGYRIVYKPVSVPGQTLETFVGADINTIVMTNLLSGMDYNVKIFASQASGFSDALT
  GLVQTLFLGVTDLQANQVEMTSLCARWQIHRHATAYRIVLESLQDTQAQESTVGGGVNRHCFYGLQPDSE
  YKISVYTKLQELEGPSVSIMQKTQSLPTEPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDDNFNKII
  NFLYSTVGALDKIGADGTQVAMVQFTDDPRTEFKLDSYKTKETLLDAIRHISYKGGNTKTGKAIKHVRDT
  LFTSDSGTRRGIPKVIVVITDGRSQDDVNKISREMQADGFNIFAIGVADADYSELVQIGSKPSSRHVFFV
  DDFDAFKKIEDELITFVCETASATCPMVHKDGVDLAGFKMMEMFGLVEKDFSAVEGVSMEPGTFNLFPCY
  QIHKDALVSQPTKYLHPEGLPSDYTMSFLFRILPDTPQEPFALWEILNKNSEPLVGIILDNGGKTLTYFN
  YDYTGDFQTVTFEGPDIRKMFYGSFHKLHVVVSKTLAKVVVDCKEVGQKAINASANITSDGVEVLGRMVR
  SRGPNGNSAPFQLQMFDIVCSTSWASKDRCCELPGLRDEESCPDLPRSCSCSETNEVALGPAGPPGGPGL
  RGPKGQQGEQGPKGPEGPRGETGPAGPQGPPGPQGPSGLSIQGMPGMPGDKGDKGDAGLPGPQGVPGGVG
  SPGRDGSPGQRGFPGKDGSSGPPGPPGPIGIPGAPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQS
  QAMVRAVARQVCEQLIQSHMARYTAILNQIPSQSSSIRTIQGPPGEPGRPGSPGTPGEQGPPGTPGFPGN
  AGVPGTPGERGLTGVKGEKGNPGIGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGP
  SGQPGYCDPSSCSAYGVGVSHPDQPEFTPVQDEQEAMDLWSAGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181277
• ORF Size: 5382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181277.4
• RefSeq Size: 6465 bp
• RefSeq ORF: 5385 bp
• Locus ID: 12818
• Cytogenetics: 15 D1
• MW: 193.2 kDa
• Gene Summary: Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors (By similarity).[UniProtKB/Swiss-Prot Function]