Itprip (NM_001001738) Mouse Tagged ORF Clone

CAT#: MR218820

Itprip (Myc-DDK-tagged) - Mouse inositol 1,4,5-triphosphate receptor interacting protein (Itprip)

Product Images

Circular map for MR218820

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itprip
• Synonyms: 4833424O12Rik; DANGER; mKIAA1754
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218820 representing NM_001001738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGAACTCTTCCGGGTCTGTCTGGTCGTGGTGACAGCCATCATTAACCACCCTCTGCTGTTCC
  CACGGGAGAATGCCACGATTCCCGAGAACGAGGAAGAGATCATACGCAAGATGCAGGAGCACCAGGAGAA
  GCTGCGCCTGGAGCAGCTACGCCTGGAGGAGGAGGTGTCACGTCTGGAGGCCGAGAAGGAGGCTCTGCGG
  CAGGTGGAGGAGGAGCAGCAGCAGCTAGAGGCCCACACCGCCTGGGACCTCTGGACCACACTCTGCATGG
  TCCTCTTCCTGATCATCGAGGTGTTGCGACAGAACCATCAGGAGGGCACCTTCCCGGAGTGCCTGGGTGG
  AGACGAGGACGAGCTGTCGGGACTAGGGGGCACGCTGCTGCAGGGCCTTCCCCTGCCCAATAGGGCCACC
  CTGGACCACTTCTACGAGCACTGTATCCGGAGCACCACGGGGGATGCCACCCGCACCCAGGAGTTTGTGG
  AAGGTTTCGTGGATGATCTGCTGGAAGCCCTGAGGAGCACCTACAACGGGAAAACCGACATGGAATTGGA
  GGACTTCATCGGCGTGGGCAGCATGTATGAGAACTGGCAAGTGGAGAGGCCACTACGGTGCCACTTGTTT
  ATACCCTTCATACCCCCAGAGCCCTACAGCTTCCACCCAGAGTTCTGGTGCTCCAGCCTTTCAACGCCCC
  TGGAACGTCAGGGCTATGGCCAGATCAAGGTGACGCTGGCTGACGGGAACCCTCTAGGCTGTGTCTGCGG
  CAAGGCTAAGCTTGAGGAGGATATGTTGTGTCTCCTCTACGGCAAGAATAGAGGGGCGTGGCCCAGCAGT
  GCCGGGTGCGGTGAGATGGAGGGCTTGCTGTGTTCCAGAGAGTCCTCCTATCTCGATGTCATGCAGGTCA
  TGAAGTGGTTTCAGATGGCCCTCACCAGAGCGTGGCACCGCATCGCCCACAAGTATGAGTTTGACCTTGC
  CTTTGGAGAGCTGGACACCCCAGGGTCTCTCAAAATCAAGTTCCGCTCGGGGAAGTCCATGCCTTTCATC
  TTGACTCCTGTGATCCAGTGTAATGACTCAGACCTGTACTTCATCTTACAACTTCCCAAGGAGCCCTGTG
  GGGGAGGCCCAGCCTCCAGCGCTCACTGGCTCCTGTCCTTTGCTGTCTATGAGCGAGAGTTCCTCCGGAT
  GACAGGCAAGGCTTTGCCGGAGGGTGCCTGCCACCTGAGCTGTTTGCAGATCGCCTCCTTCTTGCTCTCC
  AAGCAGACCCGTCTGACGGGTCCCAGTGGGCTGAGTGACTACCACCTAAAGACTGCCCTGCTGCATCTCC
  TGCTGTCCCGGCAGGCCTCTGACTGGAAGGCCAGCAAGCTGGATGTACGCCTGCAGGACCTCTTCTGCTT
  CCTGGAGAGGAGCCTGCTGGAGAAGAAACTCTATCACTTCTTCATGGGCAACCACAAGGTGCCTGAGGCT
  CTGGGTCTCCCAGAGGTTGTGCGCAGAGCCGAGCCTCTCAATCTCTTCCGGCCCTTCGTCTTGCAGCGGA
  CTCTTTACCGGAACACAGTGGACTCCTTCTATGAAATGCTTAAGAACGCCCCGGCGCTCATTAGCGAGTA
  TTCCCTCCATGTCCCTTCAGTTCGTGCCAGCCCACCACCAAAAGCTGTCGTCTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218820 representing NM_001001738
  Red=Cloning site Green=Tags(s)
  
  MAMELFRVCLVVVTAIINHPLLFPRENATIPENEEEIIRKMQEHQEKLRLEQLRLEEEVSRLEAEKEALR
  QVEEEQQQLEAHTAWDLWTTLCMVLFLIIEVLRQNHQEGTFPECLGGDEDELSGLGGTLLQGLPLPNRAT
  LDHFYEHCIRSTTGDATRTQEFVEGFVDDLLEALRSTYNGKTDMELEDFIGVGSMYENWQVERPLRCHLF
  IPFIPPEPYSFHPEFWCSSLSTPLERQGYGQIKVTLADGNPLGCVCGKAKLEEDMLCLLYGKNRGAWPSS
  AGCGEMEGLLCSRESSYLDVMQVMKWFQMALTRAWHRIAHKYEFDLAFGELDTPGSLKIKFRSGKSMPFI
  LTPVIQCNDSDLYFILQLPKEPCGGGPASSAHWLLSFAVYEREFLRMTGKALPEGACHLSCLQIASFLLS
  KQTRLTGPSGLSDYHLKTALLHLLLSRQASDWKASKLDVRLQDLFCFLERSLLEKKLYHFFMGNHKVPEA
  LGLPEVVRRAEPLNLFRPFVLQRTLYRNTVDSFYEMLKNAPALISEYSLHVPSVRASPPPKAVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001738
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001738.3
• RefSeq Size: 3781 bp
• RefSeq ORF: 1668 bp
• Locus ID: 414801
• UniProt ID: Q3TNL8
• Cytogenetics: 19 D1
• MW: 63.7 kDa
• Gene Summary: Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release.[UniProtKB/Swiss-Prot Function]