Prkacb (NM_001164198) Mouse Tagged ORF Clone

CAT#: MR218582

Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 2

Product Images

Circular map for MR218582

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkacb
• Synonyms: CbPKA; Pkacb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218582 representing NM_001164198
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTGAAAGAGTTTCTAGCCAAAGCCAAAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGA
  GTAATGCTGGGCTTGAGGATTTTGAGAGGAAGAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTT
  GGTGAAGCATAAAGCCACTGAGCAGTACTACGCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTG
  AAGCAAATAGAGCACACTCTGAATGAGAAGAGAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGC
  TGGAGTACTCTTTTAAGGATAATTCTAATTTATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTT
  CTCACATCTGAGAAGAATTGGAAGGTTCAGTGAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTA
  ACATTTGAGTACCTTCATTCCCTCGACCTCATCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACC
  ACCAGGGTTACATCCAGGTCACAGATTTCGGGTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTG
  TGGCACCCCAGAGTACCTGGCCCCGGAGATCATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGG
  GCACTGGGCGTGCTGATCTATGAGATGGCTGCTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGA
  TCTATGAGAAGATTGTCTCTGGAAAGGTCCGGTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCT
  GCGGAACCTGCTGCAGGTGGATCTGACAAAGCGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAG
  ACCCACAAGTGGTTTGCCACAACTGACTGGATTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATAC
  CAAAGTTCAGAGGCTCTGGCGATACCAGCAACTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTAT
  AACAGAAAAATGTGGAAAGGAATTTTGTGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218582 representing NM_001164198
  Red=Cloning site Green=Tags(s)
  
  MNVKEFLAKAKEDFLRKWENPPPSNAGLEDFERKKTLGTGSFGRVMLVKHKATEQYYAMKILDKQKVVKL
  KQIEHTLNEKRILQAVEFPFLVRLEYSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVL
  TFEYLHSLDLIYRDLKPENLLIDHQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWW
  ALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVSDIK
  THKWFATTDWIAIYQRKVEAPFIPKFRGSGDTSNFDDYEEEEIRVSITEKCGKEFCEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164198
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164198.1, NP_001157670.1
• RefSeq Size: 4337 bp
• RefSeq ORF: 1017 bp
• Locus ID: 18749
• UniProt ID: P68181
• Cytogenetics: 3 H2
• MW: 39.9 kDa
• Gene Summary: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function]