H2-Ke6 (NM_013543) Mouse Tagged ORF Clone

CAT#: MR218574

H2 (Myc-DDK-tagged) - Mouse H2-K region expressed gene 6 (H2-Ke6)

Specifications

Product Data

• Product Name: H2-Ke6 (NM_013543) Mouse Tagged ORF Clone
• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2-Ke6
• Synonyms: D17H6S112E; H-2Ke6; Hsd17b8; Ke-6; Ke6; Ring2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218574 representing NM_013543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCAGCTCCGGCTCCGCTCTGCGCTGGCCCTGGTCACAGGTGCGGGTAGCGGCATCGGCCGTG
  CGATCAGCGTGCGCCTAGCGGCAGAGGGCGCCGCCGTGGCCGCCTGCGACCTGGACGGGGCCGCGGCACA
  GGACACGGTGCGGCTGCTGGGAAGCCCGGGGAGCGAGGACGGGGCGCCGCGCGGCAAGCACGCTGCCTTC
  CAAGCGGATGTGTCTCAGGGCCCCGCAGCCAGACGCCTGCTGGAGGAAGTGCAGGCCTGCTTTTCTCGCC
  CGCCATCTGTCGTTGTGTCCTGTGCGGGCATCACACGCGATGAGTTTCTGCTCCACATGTCAGAAGAAGA
  CTGGGACAGAGTCATAGCTGTCAACCTCAAGGGCACCTTCCTAGTCACTCAGGCTGCAGCCCAGGCTTTA
  GTGTCCAGTGGCGGTCGTGGCTCCATCATCAACATTAGTAGCATCATTGGAAAGGTGGGGAATATCGGAC
  AAACGAATTATGCGTCGTCCAAAGCAGGAGTGATTGGGCTCACCCAGACTGCGGCCCGGGAGCTTGGACG
  ACATGGAATCCGATGTAACTCGGTCCTCCCAGGGTTCATTGCAACGCCCATGACCCAGAAAATGCCAGAG
  AAAGTGAAGGACAAGGTAACTGCAATGATTCCGTTGGGACACATGGGGGACCCTGAGGATGTGGCAGATG
  TGGTTGCATTCTTGGCATCTGAAGACAGTGGATACATCACAGGGGCCTCCGTGGAAGTCAGTGGAGGTCT
  TTTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218574 representing NM_013543
  Red=Cloning site Green=Tags(s)
  
  MASQLRLRSALALVTGAGSGIGRAISVRLAAEGAAVAACDLDGAAAQDTVRLLGSPGSEDGAPRGKHAAF
  QADVSQGPAARRLLEEVQACFSRPPSVVVSCAGITRDEFLLHMSEEDWDRVIAVNLKGTFLVTQAAAQAL
  VSSGGRGSIINISSIIGKVGNIGQTNYASSKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKMPE
  KVKDKVTAMIPLGHMGDPEDVADVVAFLASEDSGYITGASVEVSGGLFM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_013543
• ORF Size: 780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013543.2, NP_038571.2
• RefSeq Size: 981
• RefSeq ORF: 780
• Locus ID: 14979
• Cytogenetics: 17 17.98 cM
• MW: 26.6 kDa